Restriction map for
pF1KB8120
(Linear) (Six-base) MAPSORT of: KIBB8120.seq Check: 634 from: 1 to: 1455
KIBB8120.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 24 1455
Size: 24 1431
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 276 1455
Size: 153 123 1179
Fragments arranged by size:
1179 153 123
AflIII A'CryG_T
Cuts at: 0 444 1455
Size: 444 1011
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1187 1455
Size: 1187 268
AlwNI CAG_nnn'CTG
Cuts at: 0 323 489 877 969 1455
Size: 323 166 388 92 486
Fragments arranged by size:
486 388 323 166 92
ApaI G_GGCC'C
Cuts at: 0 309 572 1141 1455
Size: 309 263 569 314
Fragments arranged by size:
569 314 309 263
ApaLI G'TGCA_C
Cuts at: 0 493 950 1455
Size: 493 457 505
Fragments arranged by size:
505 493 457
AvaI C'yCGr_G
Cuts at: 0 208 1455
Size: 208 1247
BanI G'GyrC_C
Cuts at: 0 674 784 1455
Size: 674 110 671
Fragments arranged by size:
674 671 110
BanII G_rGCy'C
Cuts at: 0 309 572 724 749 1141 1455
Size: 309 263 152 25 392 314
Fragments arranged by size:
392 314 309 263 152 25
BclI T'GATC_A
Cuts at: 0 1055 1145 1455
Size: 1055 90 310
Fragments arranged by size:
1055 310 90
BfrBI ATG'CAT
Cuts at: 0 585 1455
Size: 585 870
BglI GCCn_nnn'nGGC
Cuts at: 0 362 850 1455
Size: 362 488 605
Fragments arranged by size:
605 488 362
BlpI GC'TnA_GC
Cuts at: 0 718 1455
Size: 718 737
Bme1580I G_kGCm'C
Cuts at: 0 309 463 497 572 708 846 954 1141
Size: 309 154 34 75 136 138 108 187
Cuts at: 1141 1455
Size: 314
Fragments arranged by size:
314 309 187 154 138 136 108 75
34
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 134 1455
Size: 134 1321
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 426 499 646 1369 1455
Size: 142 284 73 147 723 86
Fragments arranged by size:
723 284 147 142 86 73
Bpu10I CC'TnA_GC
Cuts at: 0 83 641 1455
Size: 83 558 814
Fragments arranged by size:
814 558 83
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 163 1455
Size: 79 84 1292
Fragments arranged by size:
1292 84 79
BsaI GGTCTCn'nnnn_
Cuts at: 0 350 379 1455
Size: 350 29 1076
Fragments arranged by size:
1076 350 29
BsaAI yAC'GTr
Cuts at: 0 498 903 1455
Size: 498 405 552
Fragments arranged by size:
552 498 405
BsaWI w'CCGG_w
Cuts at: 0 107 1250 1455
Size: 107 1143 205
Fragments arranged by size:
1143 205 107
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 82 228 634 800 1123 1455
Size: 82 146 406 166 323 332
Fragments arranged by size:
406 332 323 166 146 82
BseYI C'CCAG_C
Cuts at: 0 764 1302 1455
Size: 764 538 153
Fragments arranged by size:
764 538 153
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 811 890 1353 1455
Size: 91 720 79 463 102
Fragments arranged by size:
720 463 102 91 79
BsiHKAI G_wGCw'C
Cuts at: 0 412 497 749 954 963 1455
Size: 412 85 252 205 9 492
Fragments arranged by size:
492 412 252 205 85 9
BsmI GAATG_Cn'
Cuts at: 0 145 1455
Size: 145 1310
Bsp1286I G_dGCh'C
Cuts at: 0 309 412 463 497 572 708 724 749
Size: 309 103 51 34 75 136 16 25
Cuts at: 749 846 954 963 1141 1455
Size: 97 108 9 178 314
Fragments arranged by size:
314 309 178 136 108 103 97 75
51 34 25 16 9
BspEI T'CCGG_A
Cuts at: 0 1250 1455
Size: 1250 205
BspHI T'CATG_A
Cuts at: 0 224 1052 1455
Size: 224 828 403
Fragments arranged by size:
828 403 224
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 24 1455
Size: 24 1431
BsrBI CCG'CTC
Cuts at: 0 5 504 672 1455
Size: 5 499 168 783
Fragments arranged by size:
783 499 168 5
BsrDI GCAATG_nn'
Cuts at: 0 291 1455
Size: 291 1164
BsrFI r'CCGG_y
Cuts at: 0 1118 1455
Size: 1118 337
BssSI C'ACGA_G
Cuts at: 0 1005 1455
Size: 1005 450
BstAPI GCAn_nnn'nTGC
Cuts at: 0 492 1455
Size: 492 963
BstXI CCAn_nnnn'nTGG
Cuts at: 0 36 1095 1455
Size: 36 1059 360
Fragments arranged by size:
1059 360 36
BstYI r'GATC_y
Cuts at: 0 401 995 1239 1455
Size: 401 594 244 216
Fragments arranged by size:
594 401 244 216
Bsu36I CC'TnA_GG
Cuts at: 0 468 1455
Size: 468 987
BtsI GCAGTG_nn'
Cuts at: 0 967 1455
Size: 967 488
EaeI y'GGCC_r
Cuts at: 0 38 530 887 1211 1340 1455
Size: 38 492 357 324 129 115
Fragments arranged by size:
492 357 324 129 115 38
EarI CTCTTCn'nnn_
Cuts at: 0 926 1405 1455
Size: 926 479 50
Fragments arranged by size:
926 479 50
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1008 1455
Size: 1008 447
EcoICRI GAG'CTC
Cuts at: 0 747 1455
Size: 747 708
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 153 276 426 499 646 1369 1455
Size: 142 11 123 150 73 147 723 86
Fragments arranged by size:
723 150 147 142 123 86 73 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 157 467 644 1402 1455
Size: 157 310 177 758 53
Fragments arranged by size:
758 310 177 157 53
EcoO109I rG'GnC_Cy
Cuts at: 0 305 649 874 1137 1138 1194 1227 1455
Size: 305 344 225 263 1 56 33 228
Fragments arranged by size:
344 305 263 228 225 56 33 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1428 1455
Size: 1428 27
FspI TGC'GCA
Cuts at: 0 907 1455
Size: 907 548
FspAI rTGC'GCAy
Cuts at: 0 907 1455
Size: 907 548
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 925 957 1455
Size: 925 32 498
Fragments arranged by size:
925 498 32
MluI A'CGCG_T
Cuts at: 0 444 1455
Size: 444 1011
MscI TGG'CCA
Cuts at: 0 40 532 889 1213 1342 1455
Size: 40 492 357 324 129 113
Fragments arranged by size:
492 357 324 129 113 40
MslI CAynn'nnrTG
Cuts at: 0 34 580 1455
Size: 34 546 875
Fragments arranged by size:
875 546 34
MspA1I CmG'CkG
Cuts at: 0 489 833 1455
Size: 489 344 622
Fragments arranged by size:
622 489 344
NsiI A_TGCA'T
Cuts at: 0 587 1455
Size: 587 868
PflMI CCAn_nnn'nTGG
Cuts at: 0 132 1455
Size: 132 1323
PfoI T'CCnGG_A
Cuts at: 0 614 1355 1455
Size: 614 741 100
Fragments arranged by size:
741 614 100
PmlI CAC'GTG
Cuts at: 0 498 903 1455
Size: 498 405 552
Fragments arranged by size:
552 498 405
PpuMI rG'GwC_Cy
Cuts at: 0 1227 1455
Size: 1227 228
PspOMI G'GGCC_C
Cuts at: 0 305 568 1137 1455
Size: 305 263 569 318
Fragments arranged by size:
569 318 305 263
PstI C_TGCA'G
Cuts at: 0 545 851 1238 1455
Size: 545 306 387 217
Fragments arranged by size:
545 387 306 217
PvuII CAG'CTG
Cuts at: 0 489 833 1455
Size: 489 344 622
Fragments arranged by size:
622 489 344
SacI G_AGCT'C
Cuts at: 0 749 1455
Size: 749 706
SbfI CC_TGCA'GG
Cuts at: 0 851 1238 1455
Size: 851 387 217
Fragments arranged by size:
851 387 217
ScaI AGT'ACT
Cuts at: 0 220 1455
Size: 220 1235
SexAI A'CCwGG_T
Cuts at: 0 412 1455
Size: 412 1043
SfcI C'TryA_G
Cuts at: 0 260 541 847 1234 1455
Size: 260 281 306 387 221
Fragments arranged by size:
387 306 281 260 221
SmlI C'TyrA_G
Cuts at: 0 94 178 1455
Size: 94 84 1277
Fragments arranged by size:
1277 94 84
SspI AAT'ATT
Cuts at: 0 201 1455
Size: 201 1254
StuI AGG'CCT
Cuts at: 0 853 1455
Size: 853 602
StyI C'CwwG_G
Cuts at: 0 725 1455
Size: 725 730
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 332 672 769 778 1455
Size: 332 340 97 9 677
Fragments arranged by size:
677 340 332 97 9
TatI w'GTAC_w
Cuts at: 0 218 1455
Size: 218 1237
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 277 732 1455
Size: 277 455 723
Fragments arranged by size:
723 455 277
Enzymes that do cut:
AarI AcuI AflIII AlfI AlwNI ApaI ApaLI AvaI
BanI BanII BclI BfrBI BglI BlpI Bme1580I BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtsI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
FspI FspAI Hin4I MluI MscI MslI MspA1I NsiI
PflMI PfoI PmlI PpuMI PspOMI PstI PvuII SacI
SbfI ScaI SexAI SfcI SmlI SspI StuI StyI
TaqII TatI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BbvCI BcgI BciVI BglII BmgBI
BmrI BmtI BsaBI BsaHI BsaXI BsiEI BsiWI BsmBI
BsrGI BssHII BstBI BstEII BstZ17I BtgI BtgZI ClaI
CspCI DraI DraIII DrdI EagI EcoRI EcoRV FseI
HaeII HincII HindIII HpaI KasI KpnI MfeI MmeI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NspI PacI PasI PciI PmeI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SwaI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI