Restriction map for
pF1KB8116
(Linear) (Six-base) MAPSORT of: KIBB8116.seq Check: 1579 from: 1 to: 759
KIBB8116.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AccI GT'mk_AC
Cuts at: 0 127 759
Size: 127 632
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 611 759
Size: 611 148
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 118 759
Size: 118 641
AlwNI CAG_nnn'CTG
Cuts at: 0 534 649 759
Size: 534 115 110
Fragments arranged by size:
534 115 110
ApaI G_GGCC'C
Cuts at: 0 548 759
Size: 548 211
BanI G'GyrC_C
Cuts at: 0 62 759
Size: 62 697
BanII G_rGCy'C
Cuts at: 0 123 548 759
Size: 123 425 211
Fragments arranged by size:
425 211 123
BbsI GAAGACnn'nnnn_
Cuts at: 0 651 759
Size: 651 108
Bme1580I G_kGCm'C
Cuts at: 0 548 759
Size: 548 211
BmrI ACTGGGnnnn_n'
Cuts at: 0 335 759
Size: 335 424
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 700 721 759
Size: 211 489 21 38
Fragments arranged by size:
489 211 38 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 358 759
Size: 358 401
BseYI C'CCAG_C
Cuts at: 0 157 710 759
Size: 157 553 49
Fragments arranged by size:
553 157 49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 759
Size: 550 209
BsiHKAI G_wGCw'C
Cuts at: 0 485 759
Size: 485 274
Bsp1286I G_dGCh'C
Cuts at: 0 123 485 548 759
Size: 123 362 63 211
Fragments arranged by size:
362 211 123 63
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 239 759
Size: 239 520
BsrDI GCAATG_nn'
Cuts at: 0 504 759
Size: 504 255
BstYI r'GATC_y
Cuts at: 0 423 759
Size: 423 336
Bsu36I CC'TnA_GG
Cuts at: 0 353 759
Size: 353 406
BtgI C'CryG_G
Cuts at: 0 114 759
Size: 114 645
BtsI GCAGTG_nn'
Cuts at: 0 254 380 759
Size: 254 126 379
Fragments arranged by size:
379 254 126
EaeI y'GGCC_r
Cuts at: 0 111 538 759
Size: 111 427 221
Fragments arranged by size:
427 221 111
EarI CTCTTCn'nnn_
Cuts at: 0 698 755 759
Size: 698 57 4
Fragments arranged by size:
698 57 4
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 611 700 721 759
Size: 211 400 89 21 38
Fragments arranged by size:
400 211 89 38 21
EcoO109I rG'GnC_Cy
Cuts at: 0 357 514 544 545 759
Size: 357 157 30 1 214
Fragments arranged by size:
357 214 157 30 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 420 452 759
Size: 420 32 307
Fragments arranged by size:
420 307 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 759
Size: 128 631
MscI TGG'CCA
Cuts at: 0 113 540 759
Size: 113 427 219
Fragments arranged by size:
427 219 113
MspA1I CmG'CkG
Cuts at: 0 447 531 759
Size: 447 84 228
Fragments arranged by size:
447 228 84
NcoI C'CATG_G
Cuts at: 0 114 759
Size: 114 645
NspI r_CATG'y
Cuts at: 0 206 759
Size: 206 553
PasI CC'CwG_GG
Cuts at: 0 37 759
Size: 37 722
PflMI CCAn_nnn'nTGG
Cuts at: 0 609 759
Size: 609 150
PfoI T'CCnGG_A
Cuts at: 0 716 759
Size: 716 43
PpuMI rG'GwC_Cy
Cuts at: 0 514 759
Size: 514 245
PspOMI G'GGCC_C
Cuts at: 0 544 759
Size: 544 215
PstI C_TGCA'G
Cuts at: 0 85 320 668 759
Size: 85 235 348 91
Fragments arranged by size:
348 235 91 85
PvuII CAG'CTG
Cuts at: 0 447 759
Size: 447 312
SapI GCTCTTCn'nnn_
Cuts at: 0 755 759
Size: 755 4
SfcI C'TryA_G
Cuts at: 0 81 316 664 759
Size: 81 235 348 95
Fragments arranged by size:
348 235 95 81
SphI G_CATG'C
Cuts at: 0 206 759
Size: 206 553
StyI C'CwwG_G
Cuts at: 0 114 759
Size: 114 645
Enzymes that do cut:
AccI AcuI AlfI AlwNI ApaI BanI BanII BbsI
Bme1580I BmrI BpmI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspMI BsrDI BstYI Bsu36I BtgI BtsI EaeI EarI
Eco57MI EcoO109I Hin4I MmeI MscI MspA1I NcoI NspI
PasI PflMI PfoI PpuMI PspOMI PstI PvuII SapI
SfcI SphI StyI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MslI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SrfI SspI StuI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI