Restriction map for
pF1KB8110
(Linear) (Six-base) MAPSORT of: KIBB8110.seq Check: 9840 from: 1 to: 540
KIBB8110.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 540
Size: 171 369
AlwNI CAG_nnn'CTG
Cuts at: 0 140 540
Size: 140 400
ApaI G_GGCC'C
Cuts at: 0 510 540
Size: 510 30
AvaI C'yCGr_G
Cuts at: 0 294 490 540
Size: 294 196 50
Fragments arranged by size:
294 196 50
AvrII C'CTAG_G
Cuts at: 0 174 540
Size: 174 366
BamHI G'GATC_C
Cuts at: 0 424 540
Size: 424 116
BanI G'GyrC_C
Cuts at: 0 309 436 540
Size: 309 127 104
Fragments arranged by size:
309 127 104
BanII G_rGCy'C
Cuts at: 0 491 510 540
Size: 491 19 30
Fragments arranged by size:
491 30 19
BbvCI CC'TCA_GC
Cuts at: 0 533 540
Size: 533 7
BglI GCCn_nnn'nGGC
Cuts at: 0 137 540
Size: 137 403
BlpI GC'TnA_GC
Cuts at: 0 391 540
Size: 391 149
Bme1580I G_kGCm'C
Cuts at: 0 295 314 510 540
Size: 295 19 196 30
Fragments arranged by size:
295 196 30 19
BmrI ACTGGGnnnn_n'
Cuts at: 0 255 540
Size: 255 285
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 540
Size: 307 233
Bpu10I CC'TnA_GC
Cuts at: 0 485 533 540
Size: 485 48 7
Fragments arranged by size:
485 48 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 229 259 540
Size: 229 30 281
Fragments arranged by size:
281 229 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 319 540
Size: 319 221
BseYI C'CCAG_C
Cuts at: 0 428 540
Size: 428 112
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 424 540
Size: 328 96 116
Fragments arranged by size:
328 116 96
Bsp1286I G_dGCh'C
Cuts at: 0 295 314 491 510 540
Size: 295 19 177 19 30
Fragments arranged by size:
295 177 30 19 19
BssSI C'ACGA_G
Cuts at: 0 301 382 540
Size: 301 81 158
Fragments arranged by size:
301 158 81
BstXI CCAn_nnnn'nTGG
Cuts at: 0 42 540
Size: 42 498
BstYI r'GATC_y
Cuts at: 0 338 424 540
Size: 338 86 116
Fragments arranged by size:
338 116 86
EarI CTCTTCn'nnn_
Cuts at: 0 518 540
Size: 518 22
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 307 540
Size: 171 136 233
Fragments arranged by size:
233 171 136
EcoO109I rG'GnC_Cy
Cuts at: 0 9 495 506 507 540
Size: 9 486 11 1 33
Fragments arranged by size:
486 33 11 9 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 136 168 540
Size: 136 32 372
Fragments arranged by size:
372 136 32
MspA1I CmG'CkG
Cuts at: 0 137 540
Size: 137 403
NspI r_CATG'y
Cuts at: 0 255 540
Size: 255 285
PasI CC'CwG_GG
Cuts at: 0 314 502 540
Size: 314 188 38
Fragments arranged by size:
314 188 38
PflMI CCAn_nnn'nTGG
Cuts at: 0 126 540
Size: 126 414
PpuMI rG'GwC_Cy
Cuts at: 0 9 495 540
Size: 9 486 45
Fragments arranged by size:
486 45 9
PspOMI G'GGCC_C
Cuts at: 0 506 540
Size: 506 34
SfcI C'TryA_G
Cuts at: 0 192 348 540
Size: 192 156 192
Fragments arranged by size:
192 192 156
SmaI CCC'GGG
Cuts at: 0 296 540
Size: 296 244
SphI G_CATG'C
Cuts at: 0 255 540
Size: 255 285
SrfI GCCC'GGGC
Cuts at: 0 296 540
Size: 296 244
StuI AGG'CCT
Cuts at: 0 361 540
Size: 361 179
StyI C'CwwG_G
Cuts at: 0 174 540
Size: 174 366
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 72 133 454 540
Size: 72 61 321 86
Fragments arranged by size:
321 86 72 61
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 229 261 540
Size: 229 32 279
Fragments arranged by size:
279 229 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 66 228 466 540
Size: 66 162 238 74
Fragments arranged by size:
238 162 74 66
XmaI C'CCGG_G
Cuts at: 0 294 540
Size: 294 246
Enzymes that do cut:
AcuI AlwNI ApaI AvaI AvrII BamHI BanI BanII
BbvCI BglI BlpI Bme1580I BmrI BpmI Bpu10I BsaXI
BseRI BseYI BsgI Bsp1286I BssSI BstXI BstYI EarI
Eco57MI EcoO109I Hin4I MspA1I NspI PasI PflMI PpuMI
PspOMI SfcI SmaI SphI SrfI StuI StyI TaqII
TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI BaeI BbeI BbsI BcgI BciVI BclI
BfrBI BglII BmgBI BmtI BplI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SspI SwaI TatI Tth111I XbaI XhoI XmnI
ZraI