(Linear) (Six-base) MAPSORT of: KIBB8106.seq  Check: 7893  from: 1  to: 531



KIBB8106.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     411     531 
   Size:        411     120
 
AccI GT'mk_AC

Cuts at:      0      31     203     531 
   Size:         31     172     328

  Fragments arranged by size: 

                 328     172      31
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     346     378     531 
   Size:        346      32     153

  Fragments arranged by size: 

                 346     153      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     312     489     531 
   Size:        312     177      42

  Fragments arranged by size: 

                 312     177      42
 
BbvCI CC'TCA_GC

Cuts at:      0     313     531 
   Size:        313     218
 
BglII A'GATC_T

Cuts at:      0     219     531 
   Size:        219     312
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     101     531 
   Size:        101     430
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     364     531 
   Size:        364     167
 
Bpu10I CC'TnA_GC

Cuts at:      0     313     531 
   Size:        313     218
 
BsaBI GATnn'nnATC

Cuts at:      0      68     531 
   Size:         68     463
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     346     376     531 
   Size:        346      30     155

  Fragments arranged by size: 

                 346     155      30
 
BseYI C'CCAG_C

Cuts at:      0     416     531 
   Size:        416     115
 
BsiHKAI G_wGCw'C

Cuts at:      0     339     531 
   Size:        339     192
 
Bsp1286I G_dGCh'C

Cuts at:      0     339     531 
   Size:        339     192
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     411     531 
   Size:        411     120
 
BstYI r'GATC_y

Cuts at:      0     219     531 
   Size:        219     312
 
BstZ17I GTA'TAC

Cuts at:      0      32     204     531 
   Size:         32     172     327

  Fragments arranged by size: 

                 327     172      32
 
BtsI GCAGTG_nn'

Cuts at:      0     337     531 
   Size:        337     194
 
DraIII CAC_nnn'GTG

Cuts at:      0     435     531 
   Size:        435      96
 
EarI CTCTTCn'nnn_

Cuts at:      0     295     531 
   Size:        295     236
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     364     531 
   Size:        364     167
 
HaeII r_GCGC'y

Cuts at:      0      10     531 
   Size:         10     521
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     383     415     465     497     531 
   Size:        383      32      50      32      34

  Fragments arranged by size: 

                 383      50      34      32      32
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     423     531 
   Size:        423     108
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     346     378     531 
   Size:        346      32     153

  Fragments arranged by size: 

                 346     153      32
 
PstI C_TGCA'G

Cuts at:      0     494     531 
   Size:        494      37
 
ScaI AGT'ACT

Cuts at:      0      88     531 
   Size:         88     443
 
SfcI C'TryA_G

Cuts at:      0     490     531 
   Size:        490      41
 
StuI AGG'CCT

Cuts at:      0     127     191     531 
   Size:        127      64     340

  Fragments arranged by size: 

                 340     127      64
 
TatI w'GTAC_w

Cuts at:      0      86     531 
   Size:         86     445
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     153     185     531 
   Size:        153      32     346

  Fragments arranged by size: 

                 346     153      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     477     531 
   Size:        477      54
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      51     531 
   Size:         51     480
 
XmnI GAAnn'nnTTC

Cuts at:      0     142     531 
   Size:        142     389
 

 Enzymes that do cut:

     AarI     AccI     AloI    AlwNI    BbvCI    BglII     BmrI     BpmI
   Bpu10I    BsaBI    BsaXI    BseYI  BsiHKAI Bsp1286I    BspMI    BstYI
  BstZ17I     BtsI   DraIII     EarI  Eco57MI    HaeII    Hin4I    PflMI
     PpiI     PstI     ScaI     SfcI     StuI     TatI     TstI  Tth111I
     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I
    BmgBI     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaHI    BsaWI
    BseRI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StyI     SwaI    TaqII     XbaI     XhoI
     XmaI     ZraI