(Linear) (Six-base) MAPSORT of: KIBB8105.seq  Check: 9922  from: 1  to: 351



KIBB8105.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

BanI G'GyrC_C

Cuts at:      0     120     351 
   Size:        120     231
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     308     351 
   Size:        308      43
 
Bme1580I G_kGCm'C

Cuts at:      0     155     351 
   Size:        155     196
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     204     351 
   Size:        204     147
 
BsaHI Gr'CG_yC

Cuts at:      0     221     351 
   Size:        221     130
 
BsaWI w'CCGG_w

Cuts at:      0      62      75     191     351 
   Size:         62      13     116     160

  Fragments arranged by size: 

                 160     116      62      13
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     237     267     351 
   Size:        237      30      84

  Fragments arranged by size: 

                 237      84      30
 
Bsp1286I G_dGCh'C

Cuts at:      0     155     351 
   Size:        155     196
 
BspEI T'CCGG_A

Cuts at:      0      62      75     191     351 
   Size:         62      13     116     160

  Fragments arranged by size: 

                 160     116      62      13
 
BspHI T'CATG_A

Cuts at:      0     294     351 
   Size:        294      57
 
BstYI r'GATC_y

Cuts at:      0      59     351 
   Size:         59     292
 
Bsu36I CC'TnA_GG

Cuts at:      0     215     351 
   Size:        215     136
 
BtgI C'CryG_G

Cuts at:      0     269     351 
   Size:        269      82
 
EaeI y'GGCC_r

Cuts at:      0     171     351 
   Size:        171     180
 
EarI CTCTTCn'nnn_

Cuts at:      0     190     351 
   Size:        190     161
 
EcoO109I rG'GnC_Cy

Cuts at:      0      84     351 
   Size:         84     267
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      44      76     351 
   Size:         44      32     275

  Fragments arranged by size: 

                 275      44      32
 
HincII GTy'rAC

Cuts at:      0     240     351 
   Size:        240     111
 
MscI TGG'CCA

Cuts at:      0     173     351 
   Size:        173     178
 
NcoI C'CATG_G

Cuts at:      0     269     351 
   Size:        269      82
 
PpuMI rG'GwC_Cy

Cuts at:      0      84     351 
   Size:         84     267
 
SanDI GG'GwC_CC

Cuts at:      0      84     351 
   Size:         84     267
 
SfcI C'TryA_G

Cuts at:      0     324     351 
   Size:        324      27
 
StyI C'CwwG_G

Cuts at:      0     269     351 
   Size:        269      82
 
TatI w'GTAC_w

Cuts at:      0      11     351 
   Size:         11     340
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     237     269     351 
   Size:        237      32      82

  Fragments arranged by size: 

                 237      82      32
 
Tth111I GACn'n_nGTC

Cuts at:      0      82     351 
   Size:         82     269
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     267     351 
   Size:        267      84
 

 Enzymes that do cut:

     BanI     BbsI Bme1580I     BsaI    BsaHI    BsaWI    BsaXI Bsp1286I
    BspEI    BspHI    BstYI   Bsu36I     BtgI     EaeI     EarI EcoO109I
    Hin4I   HincII     MscI     NcoI    PpuMI    SanDI     SfcI     StyI
     TatI     TstI  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI    BsaAI    BsaBI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MslI   MspA1I     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     XbaI     XhoI     XmaI     XmnI     ZraI