Restriction map for
pF1KB8102
(Linear) (Six-base) MAPSORT of: KIBB8102.seq Check: 4487 from: 1 to: 1251
KIBB8102.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 534 1251
Size: 534 717
AfeI AGC'GCT
Cuts at: 0 980 1251
Size: 980 271
AflIII A'CryG_T
Cuts at: 0 325 1251
Size: 325 926
AleI CACnn'nnGTG
Cuts at: 0 217 751 1031 1251
Size: 217 534 280 220
Fragments arranged by size:
534 280 220 217
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 727 1251
Size: 727 524
AlwNI CAG_nnn'CTG
Cuts at: 0 236 1251
Size: 236 1015
BanI G'GyrC_C
Cuts at: 0 472 676 808 844 1168 1251
Size: 472 204 132 36 324 83
Fragments arranged by size:
472 324 204 132 83 36
BanII G_rGCy'C
Cuts at: 0 397 649 1251
Size: 397 252 602
Fragments arranged by size:
602 397 252
BbeI G_GCGC'C
Cuts at: 0 812 1251
Size: 812 439
BfrBI ATG'CAT
Cuts at: 0 69 1251
Size: 69 1182
BglI GCCn_nnn'nGGC
Cuts at: 0 1106 1251
Size: 1106 145
Bme1580I G_kGCm'C
Cuts at: 0 641 847 1173 1251
Size: 641 206 326 78
Fragments arranged by size:
641 326 206 78
BmgBI CAC'GTC
Cuts at: 0 1029 1251
Size: 1029 222
BmrI ACTGGGnnnn_n'
Cuts at: 0 257 1251
Size: 257 994
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 847 1251
Size: 847 404
BsaI GGTCTCn'nnnn_
Cuts at: 0 935 1251
Size: 935 316
BsaBI GATnn'nnATC
Cuts at: 0 1153 1251
Size: 1153 98
BsaHI Gr'CG_yC
Cuts at: 0 809 935 1251
Size: 809 126 316
Fragments arranged by size:
809 316 126
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 217 247 1251
Size: 217 30 1004
Fragments arranged by size:
1004 217 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1036 1132 1239 1251
Size: 1036 96 107 12
Fragments arranged by size:
1036 107 96 12
BseYI C'CCAG_C
Cuts at: 0 616 1251
Size: 616 635
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 817 1251
Size: 817 434
BsiHKAI G_wGCw'C
Cuts at: 0 224 397 1028 1251
Size: 224 173 631 223
Fragments arranged by size:
631 224 223 173
BsmI GAATG_Cn'
Cuts at: 0 882 1251
Size: 882 369
Bsp1286I G_dGCh'C
Cuts at: 0 224 397 641 649 847 1028 1173 1251
Size: 224 173 244 8 198 181 145 78
Fragments arranged by size:
244 224 198 181 173 145 78 8
BspHI T'CATG_A
Cuts at: 0 344 1251
Size: 344 907
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 206 697 1251
Size: 206 491 554
Fragments arranged by size:
554 491 206
BsrBI CCG'CTC
Cuts at: 0 674 1251
Size: 674 577
BsrDI GCAATG_nn'
Cuts at: 0 92 1251
Size: 92 1159
BsrFI r'CCGG_y
Cuts at: 0 890 1251
Size: 890 361
BssSI C'ACGA_G
Cuts at: 0 268 312 1251
Size: 268 44 939
Fragments arranged by size:
939 268 44
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1161 1251
Size: 1161 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1140 1185 1251
Size: 1140 45 66
Fragments arranged by size:
1140 66 45
DraI TTT'AAA
Cuts at: 0 954 1251
Size: 954 297
DraIII CAC_nnn'GTG
Cuts at: 0 571 819 1251
Size: 571 248 432
Fragments arranged by size:
571 432 248
EaeI y'GGCC_r
Cuts at: 0 128 698 791 1098 1251
Size: 128 570 93 307 153
Fragments arranged by size:
570 307 153 128 93
EarI CTCTTCn'nnn_
Cuts at: 0 279 865 1216 1251
Size: 279 586 351 35
Fragments arranged by size:
586 351 279 35
EcoICRI GAG'CTC
Cuts at: 0 395 1251
Size: 395 856
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 534 847 1251
Size: 534 313 404
Fragments arranged by size:
534 404 313
EcoNI CCTnn'n_nnAGG
Cuts at: 0 732 1251
Size: 732 519
EcoO109I rG'GnC_Cy
Cuts at: 0 686 1251
Size: 686 565
HaeII r_GCGC'y
Cuts at: 0 20 604 812 982 1251
Size: 20 584 208 170 269
Fragments arranged by size:
584 269 208 170 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 187 219 281 313 1251
Size: 187 32 62 32 938
Fragments arranged by size:
938 187 62 32 32
KasI G'GCGC_C
Cuts at: 0 808 1251
Size: 808 443
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 563 1251
Size: 563 688
MscI TGG'CCA
Cuts at: 0 130 700 1100 1251
Size: 130 570 400 151
Fragments arranged by size:
570 400 151 130
MslI CAynn'nnrTG
Cuts at: 0 217 751 1031 1159 1251
Size: 217 534 280 128 92
Fragments arranged by size:
534 280 217 128 92
MspA1I CmG'CkG
Cuts at: 0 717 797 1251
Size: 717 80 454
Fragments arranged by size:
717 454 80
NarI GG'CG_CC
Cuts at: 0 809 1251
Size: 809 442
NsiI A_TGCA'T
Cuts at: 0 71 1251
Size: 71 1180
NspI r_CATG'y
Cuts at: 0 1146 1251
Size: 1146 105
PpuMI rG'GwC_Cy
Cuts at: 0 686 1251
Size: 686 565
PshAI GACnn'nnGTC
Cuts at: 0 263 1251
Size: 263 988
PsiI TTA'TAA
Cuts at: 0 359 1251
Size: 359 892
PstI C_TGCA'G
Cuts at: 0 716 986 1251
Size: 716 270 265
Fragments arranged by size:
716 270 265
PvuII CAG'CTG
Cuts at: 0 717 1251
Size: 717 534
SacI G_AGCT'C
Cuts at: 0 397 1251
Size: 397 854
SapI GCTCTTCn'nnn_
Cuts at: 0 865 1251
Size: 865 386
SexAI A'CCwGG_T
Cuts at: 0 815 1251
Size: 815 436
SfcI C'TryA_G
Cuts at: 0 712 982 1251
Size: 712 270 269
Fragments arranged by size:
712 270 269
SfoI GGC'GCC
Cuts at: 0 810 1251
Size: 810 441
SphI G_CATG'C
Cuts at: 0 1146 1251
Size: 1146 105
StuI AGG'CCT
Cuts at: 0 585 1251
Size: 585 666
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 490 630 1153 1251
Size: 490 140 523 98
Fragments arranged by size:
523 490 140 98
TatI w'GTAC_w
Cuts at: 0 662 1251
Size: 662 589
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 365 397 1251
Size: 365 32 854
Fragments arranged by size:
854 365 32
Enzymes that do cut:
AcuI AfeI AflIII AleI AlfI AlwNI BanI BanII
BbeI BfrBI BglI Bme1580I BmgBI BmrI BpmI BsaI
BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstXI
BtgZI DraI DraIII EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I KasI MmeI MscI MslI MspA1I
NarI NsiI NspI PpuMI PshAI PsiI PstI PvuII
SacI SapI SexAI SfcI SfoI SphI StuI TaqII
TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI
BglII BlpI BmtI BplI Bpu10I BpuEI BsaAI BsaWI
BsiEI BsiWI BsmBI BspEI BsrGI BssHII BstAPI BstBI
BstEII BstYI BstZ17I Bsu36I BtgI BtsI ClaI CspCI
DrdI EagI EciI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI NaeI
NcoI NdeI NgoMIV NheI NotI NruI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI
SspI StyI SwaI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI