Restriction map for
pF1KB8019
(Linear) (Six-base) MAPSORT of: KIBB8019.seq Check: 3510 from: 1 to: 2010
KIBB8019.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 897 2010
Size: 897 1113
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 693 1369 2010
Size: 693 676 641
Fragments arranged by size:
693 676 641
AflIII A'CryG_T
Cuts at: 0 896 1293 2010
Size: 896 397 717
Fragments arranged by size:
896 717 397
AhdI GACnn_n'nnGTC
Cuts at: 0 531 1909 2010
Size: 531 1378 101
Fragments arranged by size:
1378 531 101
AleI CACnn'nnGTG
Cuts at: 0 1294 2010
Size: 1294 716
ApaI G_GGCC'C
Cuts at: 0 989 1680 2010
Size: 989 691 330
Fragments arranged by size:
989 691 330
ApaLI G'TGCA_C
Cuts at: 0 124 2010
Size: 124 1886
ApoI r'AATT_y
Cuts at: 0 136 2010
Size: 136 1874
AvaI C'yCGr_G
Cuts at: 0 73 1203 1551 1590 1680 1839 1897 2010
Size: 73 1130 348 39 90 159 58 113
Fragments arranged by size:
1130 348 159 113 90 73 58 39
BanI G'GyrC_C
Cuts at: 0 482 1143 1746 2010
Size: 482 661 603 264
Fragments arranged by size:
661 603 482 264
BanII G_rGCy'C
Cuts at: 0 890 989 1119 1680 1847 2010
Size: 890 99 130 561 167 163
Fragments arranged by size:
890 561 167 163 130 99
BbeI G_GCGC'C
Cuts at: 0 1147 2010
Size: 1147 863
BbsI GAAGACnn'nnnn_
Cuts at: 0 1404 2010
Size: 1404 606
BbvCI CC'TCA_GC
Cuts at: 0 251 544 2010
Size: 251 293 1466
Fragments arranged by size:
1466 293 251
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 664 698 777 811 1416 1450 2010
Size: 664 34 79 34 605 34 560
Fragments arranged by size:
664 605 560 79 34 34 34
BglII A'GATC_T
Cuts at: 0 185 2010
Size: 185 1825
BlpI GC'TnA_GC
Cuts at: 0 109 607 1795 2010
Size: 109 498 1188 215
Fragments arranged by size:
1188 498 215 109
Bme1580I G_kGCm'C
Cuts at: 0 128 286 444 485 989 1680 2010
Size: 128 158 158 41 504 691 330
Fragments arranged by size:
691 504 330 158 158 128 41
BmgBI CAC'GTC
Cuts at: 0 918 1272 2010
Size: 918 354 738
Fragments arranged by size:
918 738 354
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1231 1446 2010
Size: 1231 215 564
Fragments arranged by size:
1231 564 215
Bpu10I CC'TnA_GC
Cuts at: 0 251 544 2010
Size: 251 293 1466
Fragments arranged by size:
1466 293 251
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 577 1219 2010
Size: 337 240 642 791
Fragments arranged by size:
791 642 337 240
BsaI GGTCTCn'nnnn_
Cuts at: 0 1 237 488 2010
Size: 1 236 251 1522
Fragments arranged by size:
1522 251 236 1
BsaAI yAC'GTr
Cuts at: 0 1296 2010
Size: 1296 714
BsaBI GATnn'nnATC
Cuts at: 0 1243 2010
Size: 1243 767
BsaHI Gr'CG_yC
Cuts at: 0 43 447 1144 2010
Size: 43 404 697 866
Fragments arranged by size:
866 697 404 43
BsaWI w'CCGG_w
Cuts at: 0 647 2010
Size: 647 1363
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 31 61 685 715 826 856 1444 1474
Size: 31 30 624 30 111 30 588 30
Cuts at: 1474 2010
Size: 536
Fragments arranged by size:
624 588 536 111 31 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 49 52 196 475 693 2010
Size: 49 3 144 279 218 1317
Fragments arranged by size:
1317 279 218 144 49 3
BseYI C'CCAG_C
Cuts at: 0 1013 1714 2010
Size: 1013 701 296
Fragments arranged by size:
1013 701 296
BsiEI CG_ry'CG
Cuts at: 0 225 816 879 1224 1787 2010
Size: 225 591 63 345 563 223
Fragments arranged by size:
591 563 345 225 223 63
BsiHKAI G_wGCw'C
Cuts at: 0 110 128 261 631 2010
Size: 110 18 133 370 1379
Fragments arranged by size:
1379 370 133 110 18
BsmBI CGTCTCn'nnnn_
Cuts at: 0 34 815 2010
Size: 34 781 1195
Fragments arranged by size:
1195 781 34
Bsp1286I G_dGCh'C
Cuts at: 0 110 128 261 286 444 485 631 890
Size: 110 18 133 25 158 41 146 259
Cuts at: 890 989 1119 1680 1847 2010
Size: 99 130 561 167 163
Fragments arranged by size:
561 259 167 163 158 146 133 130
110 99 41 25 18
BspEI T'CCGG_A
Cuts at: 0 647 2010
Size: 647 1363
BspHI T'CATG_A
Cuts at: 0 848 2010
Size: 848 1162
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 311 897 2010
Size: 311 586 1113
Fragments arranged by size:
1113 586 311
BsrBI CCG'CTC
Cuts at: 0 321 1314 2010
Size: 321 993 696
Fragments arranged by size:
993 696 321
BsrDI GCAATG_nn'
Cuts at: 0 945 2010
Size: 945 1065
BsrFI r'CCGG_y
Cuts at: 0 116 428 662 1786 2010
Size: 116 312 234 1124 224
Fragments arranged by size:
1124 312 234 224 116
BsrGI T'GTAC_A
Cuts at: 0 1286 2010
Size: 1286 724
BssSI C'ACGA_G
Cuts at: 0 597 2010
Size: 597 1413
BstYI r'GATC_y
Cuts at: 0 185 1214 1419 2010
Size: 185 1029 205 591
Fragments arranged by size:
1029 591 205 185
BtgI C'CryG_G
Cuts at: 0 65 152 416 617 816 1048 1800 2010
Size: 65 87 264 201 199 232 752 210
Fragments arranged by size:
752 264 232 210 201 199 87 65
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1230 2010
Size: 1230 780
BtsI GCAGTG_nn'
Cuts at: 0 1405 1656 1728 1734 2010
Size: 1405 251 72 6 276
Fragments arranged by size:
1405 276 251 72 6
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 798 833 1632 1667 2010
Size: 798 35 799 35 343
Fragments arranged by size:
799 798 343 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 1753 2010
Size: 1753 257
EaeI y'GGCC_r
Cuts at: 0 89 222 371 866 876 1004 1784 1788
Size: 89 133 149 495 10 128 780 4
Cuts at: 1788 2010
Size: 222
Fragments arranged by size:
780 495 222 149 133 128 89 10
4
EagI C'GGCC_G
Cuts at: 0 222 876 1784 2010
Size: 222 654 908 226
Fragments arranged by size:
908 654 226 222
EarI CTCTTCn'nnn_
Cuts at: 0 1133 1153 2010
Size: 1133 20 857
Fragments arranged by size:
1133 857 20
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 681 706 1282 2010
Size: 19 662 25 576 728
Fragments arranged by size:
728 662 576 25 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 693 1231 1369 1446 2010
Size: 693 538 138 77 564
Fragments arranged by size:
693 564 538 138 77
EcoNI CCTnn'n_nnAGG
Cuts at: 0 250 685 2010
Size: 250 435 1325
Fragments arranged by size:
1325 435 250
EcoO109I rG'GnC_Cy
Cuts at: 0 275 985 1017 1555 1587 1677 1903 2010
Size: 275 710 32 538 32 90 226 107
Fragments arranged by size:
710 538 275 226 107 90 32 32
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1374 1404 2010
Size: 1374 30 606
Fragments arranged by size:
1374 606 30
FseI GG_CCGG'CC
Cuts at: 0 1790 2010
Size: 1790 220
HaeII r_GCGC'y
Cuts at: 0 681 1147 1175 2010
Size: 681 466 28 835
Fragments arranged by size:
835 681 466 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 259 291 517 544 549 576 685 717
Size: 259 32 226 27 5 27 109 32
Cuts at: 717 1199 1231 1461 1493 1895 1927 2010
Size: 482 32 230 32 402 32 83
Fragments arranged by size:
482 402 259 230 226 109 83 32
32 32 32 32 27 27 5
HincII GTy'rAC
Cuts at: 0 1377 2010
Size: 1377 633
HindIII A'AGCT_T
Cuts at: 0 205 2010
Size: 205 1805
KasI G'GCGC_C
Cuts at: 0 1143 2010
Size: 1143 867
MscI TGG'CCA
Cuts at: 0 373 1006 2010
Size: 373 633 1004
Fragments arranged by size:
1004 633 373
MslI CAynn'nnrTG
Cuts at: 0 266 365 1294 2010
Size: 266 99 929 716
Fragments arranged by size:
929 716 266 99
MspA1I CmG'CkG
Cuts at: 0 871 1149 1194 1497 1610 1769 2010
Size: 871 278 45 303 113 159 241
Fragments arranged by size:
871 303 278 241 159 113 45
NaeI GCC'GGC
Cuts at: 0 664 1788 2010
Size: 664 1124 222
Fragments arranged by size:
1124 664 222
NarI GG'CG_CC
Cuts at: 0 1144 2010
Size: 1144 866
NcoI C'CATG_G
Cuts at: 0 152 416 2010
Size: 152 264 1594
Fragments arranged by size:
1594 264 152
NgoMIV G'CCGG_C
Cuts at: 0 662 1786 2010
Size: 662 1124 224
Fragments arranged by size:
1124 662 224
NspI r_CATG'y
Cuts at: 0 900 1230 2010
Size: 900 330 780
Fragments arranged by size:
900 780 330
PasI CC'CwG_GG
Cuts at: 0 279 2010
Size: 279 1731
PciI A'CATG_T
Cuts at: 0 896 2010
Size: 896 1114
PflMI CCAn_nnn'nTGG
Cuts at: 0 1013 2010
Size: 1013 997
PfoI T'CCnGG_A
Cuts at: 0 1913 2010
Size: 1913 97
PmlI CAC'GTG
Cuts at: 0 1296 2010
Size: 1296 714
PpuMI rG'GwC_Cy
Cuts at: 0 275 1017 1555 1587 1903 2010
Size: 275 742 538 32 316 107
Fragments arranged by size:
742 538 316 275 107 32
PspOMI G'GGCC_C
Cuts at: 0 985 1676 2010
Size: 985 691 334
Fragments arranged by size:
985 691 334
PstI C_TGCA'G
Cuts at: 0 908 1429 2010
Size: 908 521 581
Fragments arranged by size:
908 581 521
PvuII CAG'CTG
Cuts at: 0 1194 1769 2010
Size: 1194 575 241
Fragments arranged by size:
1194 575 241
RsrII CG'GwC_CG
Cuts at: 0 693 2010
Size: 693 1317
SanDI GG'GwC_CC
Cuts at: 0 275 1017 1555 1903 2010
Size: 275 742 538 348 107
Fragments arranged by size:
742 538 348 275 107
SexAI A'CCwGG_T
Cuts at: 0 50 2010
Size: 50 1960
SfcI C'TryA_G
Cuts at: 0 904 1425 2010
Size: 904 521 585
Fragments arranged by size:
904 585 521
SfoI GGC'GCC
Cuts at: 0 1145 2010
Size: 1145 865
SgrAI Cr'CCGG_yG
Cuts at: 0 428 662 2010
Size: 428 234 1348
Fragments arranged by size:
1348 428 234
SmaI CCC'GGG
Cuts at: 0 1553 1592 1682 1841 1899 2010
Size: 1553 39 90 159 58 111
Fragments arranged by size:
1553 159 111 90 58 39
SmlI C'TyrA_G
Cuts at: 0 316 556 1234 2010
Size: 316 240 678 776
Fragments arranged by size:
776 678 316 240
StuI AGG'CCT
Cuts at: 0 1825 1873 2010
Size: 1825 48 137
Fragments arranged by size:
1825 137 48
StyI C'CwwG_G
Cuts at: 0 152 416 487 1868 2010
Size: 152 264 71 1381 142
Fragments arranged by size:
1381 264 152 142 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 500 512 2010
Size: 500 12 1498
Fragments arranged by size:
1498 500 12
TatI w'GTAC_w
Cuts at: 0 1286 2010
Size: 1286 724
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1553 1585 2010
Size: 1553 32 425
Fragments arranged by size:
1553 425 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 570 1798 2010
Size: 570 1228 212
Fragments arranged by size:
1228 570 212
XmaI C'CCGG_G
Cuts at: 0 1551 1590 1680 1839 1897 2010
Size: 1551 39 90 159 58 113
Fragments arranged by size:
1551 159 113 90 58 39
Enzymes that do cut:
AarI AcuI AflIII AhdI AleI ApaI ApaLI ApoI
AvaI BanI BanII BbeI BbsI BbvCI BcgI BglII
BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstYI BtgI BtgZI BtsI CspCI DraIII
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I FalI
FseI HaeII Hin4I HincII HindIII KasI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmlI PpuMI PspOMI PstI PvuII RsrII
SanDI SexAI SfcI SfoI SgrAI SmaI SmlI StuI
StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AlfI
AloI AlwNI AscI AseI AsiSI AvrII BaeI BamHI
BciVI BclI BfrBI BglI BmrI BmtI BplI BsgI
BsiWI BsmI BssHII BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I ClaI DraI DrdI EcoICRI EcoRI EcoRV FspI
FspAI HpaI KpnI MfeI MluI MmeI NdeI NheI
NotI NruI NsiI PacI PmeI PpiI PshAI PsiI
PspXI PsrI PvuI SacI SacII SalI SapI SbfI
ScaI SfiI SgfI SnaBI SpeI SphI SrfI SspI
SwaI Tth111I XbaI XhoI XmnI ZraI