Restriction map for
pF1KB8009
(Linear) (Six-base) MAPSORT of: KIBB8009.seq Check: 4348 from: 1 to: 1149
KIBB8009.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AatII G_ACGT'C
Cuts at: 0 79 1149
Size: 79 1070
Acc65I G'GTAC_C
Cuts at: 0 879 1149
Size: 879 270
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 515 608 907 985 1149
Size: 515 93 299 78 164
Fragments arranged by size:
515 299 164 93 78
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 812 1139 1149
Size: 812 327 10
Fragments arranged by size:
812 327 10
ApaLI G'TGCA_C
Cuts at: 0 452 1149
Size: 452 697
ApoI r'AATT_y
Cuts at: 0 567 739 908 1143 1149
Size: 567 172 169 235 6
Fragments arranged by size:
567 235 172 169 6
BanI G'GyrC_C
Cuts at: 0 879 1149
Size: 879 270
BanII G_rGCy'C
Cuts at: 0 942 1149
Size: 942 207
BbsI GAAGACnn'nnnn_
Cuts at: 0 145 467 1149
Size: 145 322 682
Fragments arranged by size:
682 322 145
BciVI GTATCCnnnnn_n'
Cuts at: 0 990 1149
Size: 990 159
BclI T'GATC_A
Cuts at: 0 819 1149
Size: 819 330
Bme1580I G_kGCm'C
Cuts at: 0 456 1149
Size: 456 693
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 704 1149
Size: 704 445
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 699 1149
Size: 209 490 450
Fragments arranged by size:
490 450 209
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 712 1149
Size: 712 437
BsaAI yAC'GTr
Cuts at: 0 63 1149
Size: 63 1086
BsaHI Gr'CG_yC
Cuts at: 0 76 1149
Size: 76 1073
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 182 212 1149
Size: 182 30 937
Fragments arranged by size:
937 182 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 266 1149
Size: 70 196 883
Fragments arranged by size:
883 196 70
BsiEI CG_ry'CG
Cuts at: 0 44 1149
Size: 44 1105
BsiHKAI G_wGCw'C
Cuts at: 0 456 801 1149
Size: 456 345 348
Fragments arranged by size:
456 348 345
BsmI GAATG_Cn'
Cuts at: 0 509 1016 1149
Size: 509 507 133
Fragments arranged by size:
509 507 133
BsmBI CGTCTCn'nnnn_
Cuts at: 0 67 1149
Size: 67 1082
Bsp1286I G_dGCh'C
Cuts at: 0 456 801 942 1149
Size: 456 345 141 207
Fragments arranged by size:
456 345 207 141
BspHI T'CATG_A
Cuts at: 0 246 1028 1149
Size: 246 782 121
Fragments arranged by size:
782 246 121
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 891 1149
Size: 891 258
BsrFI r'CCGG_y
Cuts at: 0 39 1149
Size: 39 1110
BssSI C'ACGA_G
Cuts at: 0 935 1149
Size: 935 214
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1135 1149
Size: 1135 14
BtgI C'CryG_G
Cuts at: 0 579 1149
Size: 579 570
BtsI GCAGTG_nn'
Cuts at: 0 1123 1149
Size: 1123 26
EaeI y'GGCC_r
Cuts at: 0 41 436 1149
Size: 41 395 713
Fragments arranged by size:
713 395 41
EagI C'GGCC_G
Cuts at: 0 41 1149
Size: 41 1108
EarI CTCTTCn'nnn_
Cuts at: 0 255 499 982 1030 1149
Size: 255 244 483 48 119
Fragments arranged by size:
483 255 244 119 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 515 608 699 907 985 1149
Size: 209 306 93 91 208 78 164
Fragments arranged by size:
306 209 208 164 93 91 78
EcoRV GAT'ATC
Cuts at: 0 147 1149
Size: 147 1002
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1041 1099 1149
Size: 1041 58 50
Fragments arranged by size:
1041 58 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 66 98 1149
Size: 66 32 1051
Fragments arranged by size:
1051 66 32
KpnI G_GTAC'C
Cuts at: 0 883 1149
Size: 883 266
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 1073 1149
Size: 148 925 76
Fragments arranged by size:
925 148 76
MslI CAynn'nnrTG
Cuts at: 0 794 1149
Size: 794 355
MspA1I CmG'CkG
Cuts at: 0 984 1149
Size: 984 165
NaeI GCC'GGC
Cuts at: 0 41 1149
Size: 41 1108
NcoI C'CATG_G
Cuts at: 0 579 1149
Size: 579 570
NgoMIV G'CCGG_C
Cuts at: 0 39 1149
Size: 39 1110
NspI r_CATG'y
Cuts at: 0 529 1149
Size: 529 620
PfoI T'CCnGG_A
Cuts at: 0 711 1149
Size: 711 438
PstI C_TGCA'G
Cuts at: 0 906 1149
Size: 906 243
PvuII CAG'CTG
Cuts at: 0 984 1149
Size: 984 165
SapI GCTCTTCn'nnn_
Cuts at: 0 1030 1149
Size: 1030 119
SfcI C'TryA_G
Cuts at: 0 465 902 1149
Size: 465 437 247
Fragments arranged by size:
465 437 247
SmlI C'TyrA_G
Cuts at: 0 691 1149
Size: 691 458
SphI G_CATG'C
Cuts at: 0 529 1149
Size: 529 620
SspI AAT'ATT
Cuts at: 0 646 1149
Size: 646 503
StyI C'CwwG_G
Cuts at: 0 579 1149
Size: 579 570
XmnI GAAnn'nnTTC
Cuts at: 0 704 1149
Size: 704 445
ZraI GAC'GTC
Cuts at: 0 77 1149
Size: 77 1072
Enzymes that do cut:
AatII Acc65I AcuI AlfI ApaLI ApoI BanI BanII
BbsI BciVI BclI Bme1580I BplI BpmI BpuEI BsaAI
BsaHI BsaXI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrFI BssSI BstXI BtgI BtsI EaeI
EagI EarI Eco57MI EcoRV FalI Hin4I KpnI MmeI
MslI MspA1I NaeI NcoI NgoMIV NspI PfoI PstI
PvuII SapI SfcI SmlI SphI SspI StyI XmnI
ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AflII AflIII AgeI AhdI
AleI AloI AlwNI ApaI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbvCI BcgI BfrBI BglI
BglII BlpI BmgBI BmrI BmtI Bpu10I BsaI BsaBI
BsaWI BseRI BseYI BsiWI BspEI BsrBI BsrDI BsrGI
BssHII BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EciI EcoICRI EcoNI
EcoO109I EcoRI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI MfeI MluI MscI NarI NdeI NheI
NotI NruI NsiI PacI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI StuI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI