Restriction map for
pF1KB7994
(Linear) (Six-base) MAPSORT of: KIBB7994.seq Check: 8713 from: 1 to: 1341
KIBB7994.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
Acc65I G'GTAC_C
Cuts at: 0 476 1341
Size: 476 865
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 757 764 1341
Size: 757 7 577
Fragments arranged by size:
757 577 7
AgeI A'CCGG_T
Cuts at: 0 494 574 1341
Size: 494 80 767
Fragments arranged by size:
767 494 80
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 941 1341
Size: 941 400
AlwNI CAG_nnn'CTG
Cuts at: 0 162 793 1043 1092 1341
Size: 162 631 250 49 249
Fragments arranged by size:
631 250 249 162 49
ApaI G_GGCC'C
Cuts at: 0 1286 1341
Size: 1286 55
ApaLI G'TGCA_C
Cuts at: 0 240 691 1341
Size: 240 451 650
Fragments arranged by size:
650 451 240
ApoI r'AATT_y
Cuts at: 0 334 748 1341
Size: 334 414 593
Fragments arranged by size:
593 414 334
AvaI C'yCGr_G
Cuts at: 0 38 1341
Size: 38 1303
BamHI G'GATC_C
Cuts at: 0 420 1341
Size: 420 921
BanI G'GyrC_C
Cuts at: 0 349 476 1105 1341
Size: 349 127 629 236
Fragments arranged by size:
629 349 236 127
BanII G_rGCy'C
Cuts at: 0 1286 1341
Size: 1286 55
BclI T'GATC_A
Cuts at: 0 359 1341
Size: 359 982
BfrBI ATG'CAT
Cuts at: 0 672 1341
Size: 672 669
BglI GCCn_nnn'nGGC
Cuts at: 0 543 1341
Size: 543 798
Bme1580I G_kGCm'C
Cuts at: 0 156 244 352 695 1286 1341
Size: 156 88 108 343 591 55
Fragments arranged by size:
591 343 156 108 88 55
BmrI ACTGGGnnnn_n'
Cuts at: 0 272 1186 1341
Size: 272 914 155
Fragments arranged by size:
914 272 155
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 331 852 1341
Size: 145 186 521 489
Fragments arranged by size:
521 489 186 145
BsaI GGTCTCn'nnnn_
Cuts at: 0 307 1341
Size: 307 1034
BsaBI GATnn'nnATC
Cuts at: 0 361 451 836 1341
Size: 361 90 385 505
Fragments arranged by size:
505 385 361 90
BsaHI Gr'CG_yC
Cuts at: 0 13 1330 1341
Size: 13 1317 11
Fragments arranged by size:
1317 13 11
BsaWI w'CCGG_w
Cuts at: 0 494 574 1341
Size: 494 80 767
Fragments arranged by size:
767 494 80
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 37 67 130 160 484 514 1341
Size: 37 30 63 30 324 30 827
Fragments arranged by size:
827 324 63 37 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 24 219 222 968 1341
Size: 24 195 3 746 373
Fragments arranged by size:
746 373 195 24 3
BseYI C'CCAG_C
Cuts at: 0 155 345 1341
Size: 155 190 996
Fragments arranged by size:
996 190 155
BsiHKAI G_wGCw'C
Cuts at: 0 244 695 1341
Size: 244 451 646
Fragments arranged by size:
646 451 244
BsmI GAATG_Cn'
Cuts at: 0 918 1187 1341
Size: 918 269 154
Fragments arranged by size:
918 269 154
Bsp1286I G_dGCh'C
Cuts at: 0 156 244 352 695 1286 1341
Size: 156 88 108 343 591 55
Fragments arranged by size:
591 343 156 108 88 55
BsrBI CCG'CTC
Cuts at: 0 77 1341
Size: 77 1264
BsrDI GCAATG_nn'
Cuts at: 0 56 724 1202 1341
Size: 56 668 478 139
Fragments arranged by size:
668 478 139 56
BsrFI r'CCGG_y
Cuts at: 0 494 574 1341
Size: 494 80 767
Fragments arranged by size:
767 494 80
BstAPI GCAn_nnn'nTGC
Cuts at: 0 793 1341
Size: 793 548
BstYI r'GATC_y
Cuts at: 0 420 1341
Size: 420 921
Bsu36I CC'TnA_GG
Cuts at: 0 425 1341
Size: 425 916
BtsI GCAGTG_nn'
Cuts at: 0 216 791 1341
Size: 216 575 550
Fragments arranged by size:
575 550 216
EaeI y'GGCC_r
Cuts at: 0 290 648 1199 1341
Size: 290 358 551 142
Fragments arranged by size:
551 358 290 142
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 331 757 764 852 1341
Size: 145 186 426 7 88 489
Fragments arranged by size:
489 426 186 145 88 7
EcoO109I rG'GnC_Cy
Cuts at: 0 1282 1283 1341
Size: 1282 1 58
Fragments arranged by size:
1282 58 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 130 162 1015 1047 1341
Size: 130 32 853 32 294
Fragments arranged by size:
853 294 130 32 32
KpnI G_GTAC'C
Cuts at: 0 480 1341
Size: 480 861
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 1341
Size: 415 926
MscI TGG'CCA
Cuts at: 0 292 650 1341
Size: 292 358 691
Fragments arranged by size:
691 358 292
MslI CAynn'nnrTG
Cuts at: 0 355 400 923 1341
Size: 355 45 523 418
Fragments arranged by size:
523 418 355 45
NsiI A_TGCA'T
Cuts at: 0 674 1341
Size: 674 667
NspI r_CATG'y
Cuts at: 0 1254 1341
Size: 1254 87
PasI CC'CwG_GG
Cuts at: 0 162 1341
Size: 162 1179
PflMI CCAn_nnn'nTGG
Cuts at: 0 162 345 1341
Size: 162 183 996
Fragments arranged by size:
996 183 162
PfoI T'CCnGG_A
Cuts at: 0 857 1341
Size: 857 484
PspOMI G'GGCC_C
Cuts at: 0 1282 1341
Size: 1282 59
PstI C_TGCA'G
Cuts at: 0 307 1341
Size: 307 1034
SfcI C'TryA_G
Cuts at: 0 303 1341
Size: 303 1038
SmaI CCC'GGG
Cuts at: 0 40 1341
Size: 40 1301
StyI C'CwwG_G
Cuts at: 0 317 685 1341
Size: 317 368 656
Fragments arranged by size:
656 368 317
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 154 951 1097 1146 1341
Size: 154 797 146 49 195
Fragments arranged by size:
797 195 154 146 49
TatI w'GTAC_w
Cuts at: 0 434 1341
Size: 434 907
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 484 516 1072 1104 1341
Size: 484 32 556 32 237
Fragments arranged by size:
556 484 237 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 454 1341
Size: 454 887
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 285 633 1341
Size: 285 348 708
Fragments arranged by size:
708 348 285
XmaI C'CCGG_G
Cuts at: 0 38 1341
Size: 38 1303
Enzymes that do cut:
Acc65I AcuI AgeI AlfI AlwNI ApaI ApaLI ApoI
AvaI BamHI BanI BanII BclI BfrBI BglI Bme1580I
BmrI BpmI BsaI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiHKAI BsmI Bsp1286I BsrBI BsrDI BsrFI BstAPI
BstYI Bsu36I BtsI EaeI Eco57MI EcoO109I Hin4I KpnI
MmeI MscI MslI NsiI NspI PasI PflMI PfoI
PspOMI PstI SfcI SmaI StyI TaqII TatI TstI
Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AhdI
AleI AloI AscI AseI AsiSI AvrII BaeI BbeI
BbsI BbvCI BcgI BciVI BglII BlpI BmgBI BmtI
BplI Bpu10I BpuEI BsaAI BsgI BsiEI BsiWI BsmBI
BspEI BspHI BspMI BsrGI BssHII BssSI BstBI BstEII
BstXI BstZ17I BtgI BtgZI ClaI CspCI DraI DraIII
DrdI EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI MfeI MluI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI PacI PciI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI XbaI XhoI XmnI
ZraI