Restriction map for
pF1KB7988
(Linear) (Six-base) MAPSORT of: KIBB7988.seq Check: 9773 from: 1 to: 975
KIBB7988.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AflIII A'CryG_T
Cuts at: 0 500 902 975
Size: 500 402 73
Fragments arranged by size:
500 402 73
AleI CACnn'nnGTG
Cuts at: 0 193 975
Size: 193 782
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 541 975
Size: 541 434
AlwNI CAG_nnn'CTG
Cuts at: 0 705 975
Size: 705 270
ApaI G_GGCC'C
Cuts at: 0 257 975
Size: 257 718
AvaI C'yCGr_G
Cuts at: 0 112 175 250 437 975
Size: 112 63 75 187 538
Fragments arranged by size:
538 187 112 75 63
BanI G'GyrC_C
Cuts at: 0 135 828 913 975
Size: 135 693 85 62
Fragments arranged by size:
693 135 85 62
BanII G_rGCy'C
Cuts at: 0 106 202 257 975
Size: 106 96 55 718
Fragments arranged by size:
718 106 96 55
BbeI G_GCGC'C
Cuts at: 0 139 975
Size: 139 836
BbsI GAAGACnn'nnnn_
Cuts at: 0 496 975
Size: 496 479
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 903 937 975
Size: 903 34 38
Fragments arranged by size:
903 38 34
BglI GCCn_nnn'nGGC
Cuts at: 0 217 284 975
Size: 217 67 691
Fragments arranged by size:
691 217 67
Bme1580I G_kGCm'C
Cuts at: 0 257 833 916 975
Size: 257 576 83 59
Fragments arranged by size:
576 257 83 59
BmgBI CAC'GTC
Cuts at: 0 903 975
Size: 903 72
BmrI ACTGGGnnnn_n'
Cuts at: 0 145 705 975
Size: 145 560 270
Fragments arranged by size:
560 270 145
BsaHI Gr'CG_yC
Cuts at: 0 26 136 975
Size: 26 110 839
Fragments arranged by size:
839 110 26
BsaWI w'CCGG_w
Cuts at: 0 320 862 975
Size: 320 542 113
Fragments arranged by size:
542 320 113
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 375 418 975
Size: 375 43 557
Fragments arranged by size:
557 375 43
BseYI C'CCAG_C
Cuts at: 0 90 274 623 675 850 872 975
Size: 90 184 349 52 175 22 103
Fragments arranged by size:
349 184 175 103 90 52 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 735 975
Size: 463 272 240
Fragments arranged by size:
463 272 240
BsiEI CG_ry'CG
Cuts at: 0 635 728 975
Size: 635 93 247
Fragments arranged by size:
635 247 93
BsiHKAI G_wGCw'C
Cuts at: 0 601 953 975
Size: 601 352 22
Fragments arranged by size:
601 352 22
BsmBI CGTCTCn'nnnn_
Cuts at: 0 864 975
Size: 864 111
Bsp1286I G_dGCh'C
Cuts at: 0 106 202 257 601 833 916 953 975
Size: 106 96 55 344 232 83 37 22
Fragments arranged by size:
344 232 106 96 83 55 37 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 435 975
Size: 435 540
BsrFI r'CCGG_y
Cuts at: 0 283 669 727 975
Size: 283 386 58 248
Fragments arranged by size:
386 283 248 58
BstXI CCAn_nnnn'nTGG
Cuts at: 0 747 765 975
Size: 747 18 210
Fragments arranged by size:
747 210 18
BtgI C'CryG_G
Cuts at: 0 581 692 746 975
Size: 581 111 54 229
Fragments arranged by size:
581 229 111 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 906 975
Size: 906 69
BtsI GCAGTG_nn'
Cuts at: 0 412 520 975
Size: 412 108 455
Fragments arranged by size:
455 412 108
EaeI y'GGCC_r
Cuts at: 0 285 632 725 975
Size: 285 347 93 250
Fragments arranged by size:
347 285 250 93
EagI C'GGCC_G
Cuts at: 0 632 725 975
Size: 632 93 250
Fragments arranged by size:
632 250 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 722 922 975
Size: 722 200 53
Fragments arranged by size:
722 200 53
EcoO109I rG'GnC_Cy
Cuts at: 0 129 254 975
Size: 129 125 721
Fragments arranged by size:
721 129 125
HaeII r_GCGC'y
Cuts at: 0 139 230 318 676 946 975
Size: 139 91 88 358 270 29
Fragments arranged by size:
358 270 139 91 88 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 431 463 975
Size: 431 32 512
Fragments arranged by size:
512 431 32
KasI G'GCGC_C
Cuts at: 0 135 975
Size: 135 840
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 335 782 800 975
Size: 131 204 447 18 175
Fragments arranged by size:
447 204 175 131 18
MslI CAynn'nnrTG
Cuts at: 0 193 394 975
Size: 193 201 581
Fragments arranged by size:
581 201 193
MspA1I CmG'CkG
Cuts at: 0 571 623 657 975
Size: 571 52 34 318
Fragments arranged by size:
571 318 52 34
NaeI GCC'GGC
Cuts at: 0 285 671 975
Size: 285 386 304
Fragments arranged by size:
386 304 285
NarI GG'CG_CC
Cuts at: 0 136 975
Size: 136 839
NcoI C'CATG_G
Cuts at: 0 581 692 746 975
Size: 581 111 54 229
Fragments arranged by size:
581 229 111 54
NgoMIV G'CCGG_C
Cuts at: 0 283 669 975
Size: 283 386 306
Fragments arranged by size:
386 306 283
NotI GC'GGCC_GC
Cuts at: 0 632 975
Size: 632 343
PasI CC'CwG_GG
Cuts at: 0 258 975
Size: 258 717
PfoI T'CCnGG_A
Cuts at: 0 20 975
Size: 20 955
PpuMI rG'GwC_Cy
Cuts at: 0 129 975
Size: 129 846
PspOMI G'GGCC_C
Cuts at: 0 253 975
Size: 253 722
PstI C_TGCA'G
Cuts at: 0 794 883 975
Size: 794 89 92
Fragments arranged by size:
794 92 89
PvuII CAG'CTG
Cuts at: 0 657 975
Size: 657 318
RsrII CG'GwC_CG
Cuts at: 0 730 975
Size: 730 245
SfcI C'TryA_G
Cuts at: 0 790 879 975
Size: 790 89 96
Fragments arranged by size:
790 96 89
SfoI GGC'GCC
Cuts at: 0 137 975
Size: 137 838
SgrAI Cr'CCGG_yG
Cuts at: 0 669 975
Size: 669 306
SmaI CCC'GGG
Cuts at: 0 114 177 252 975
Size: 114 63 75 723
Fragments arranged by size:
723 114 75 63
SrfI GCCC'GGGC
Cuts at: 0 252 975
Size: 252 723
StuI AGG'CCT
Cuts at: 0 539 975
Size: 539 436
StyI C'CwwG_G
Cuts at: 0 581 692 746 975
Size: 581 111 54 229
Fragments arranged by size:
581 229 111 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 445 975
Size: 445 530
Tth111I GACn'n_nGTC
Cuts at: 0 432 975
Size: 432 543
XmaI C'CCGG_G
Cuts at: 0 112 175 250 975
Size: 112 63 75 725
Fragments arranged by size:
725 112 75 63
Enzymes that do cut:
AflIII AleI AlfI AlwNI ApaI AvaI BanI BanII
BbeI BbsI BcgI BglI Bme1580I BmgBI BmrI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspMI BsrFI BstXI BtgI BtgZI BtsI EaeI EagI
EciI EcoO109I HaeII Hin4I KasI MmeI MslI MspA1I
NaeI NarI NcoI NgoMIV NotI PasI PfoI PpuMI
PspOMI PstI PvuII RsrII SfcI SfoI SgrAI SmaI
SrfI StuI StyI TaqII Tth111I XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BciVI BclI BfrBI BglII
BlpI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaXI BsiWI BsmI BspEI BspHI BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I ClaI CspCI DraI DraIII DrdI EarI EcoICRI
Eco57MI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MscI NdeI
NheI NruI NsiI NspI PacI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SmlI SnaBI SpeI SphI SspI SwaI TatI
TstI XbaI XcmI XhoI XmnI ZraI