Restriction map for
pF1KB7979
(Linear) (Six-base) MAPSORT of: KIBB7979.seq Check: 3221 from: 1 to: 1296
KIBB7979.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 0 419 1296
Size: 419 877
Acc65I G'GTAC_C
Cuts at: 0 608 1296
Size: 608 688
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 1296
Size: 43 1253
AflIII A'CryG_T
Cuts at: 0 1094 1296
Size: 1094 202
AlwNI CAG_nnn'CTG
Cuts at: 0 1047 1050 1239 1296
Size: 1047 3 189 57
Fragments arranged by size:
1047 189 57 3
ApoI r'AATT_y
Cuts at: 0 391 1296
Size: 391 905
AseI AT'TA_AT
Cuts at: 0 625 1296
Size: 625 671
BanI G'GyrC_C
Cuts at: 0 608 799 1296
Size: 608 191 497
Fragments arranged by size:
608 497 191
BbsI GAAGACnn'nnnn_
Cuts at: 0 113 1296
Size: 113 1183
BbvCI CC'TCA_GC
Cuts at: 0 1147 1296
Size: 1147 149
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 253 287 770 804 1296
Size: 253 34 483 34 492
Fragments arranged by size:
492 483 253 34 34
BclI T'GATC_A
Cuts at: 0 852 1296
Size: 852 444
BglI GCCn_nnn'nGGC
Cuts at: 0 793 1151 1296
Size: 793 358 145
Fragments arranged by size:
793 358 145
Bme1580I G_kGCm'C
Cuts at: 0 802 1296
Size: 802 494
BmgBI CAC'GTC
Cuts at: 0 918 1296
Size: 918 378
BmrI ACTGGGnnnn_n'
Cuts at: 0 381 1296
Size: 381 915
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 1216 1296
Size: 355 861 80
Fragments arranged by size:
861 355 80
Bpu10I CC'TnA_GC
Cuts at: 0 1147 1296
Size: 1147 149
BsaAI yAC'GTr
Cuts at: 0 66 1296
Size: 66 1230
BsaHI Gr'CG_yC
Cuts at: 0 731 1296
Size: 731 565
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 436 1296
Size: 436 860
BseYI C'CCAG_C
Cuts at: 0 319 1296
Size: 319 977
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 1296
Size: 229 1067
BsiEI CG_ry'CG
Cuts at: 0 1155 1296
Size: 1155 141
BsmI GAATG_Cn'
Cuts at: 0 867 1296
Size: 867 429
Bsp1286I G_dGCh'C
Cuts at: 0 802 1296
Size: 802 494
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 390 596 1296
Size: 390 206 700
Fragments arranged by size:
700 390 206
BstEII G'GTnAC_C
Cuts at: 0 684 1296
Size: 684 612
BstXI CCAn_nnnn'nTGG
Cuts at: 0 825 1296
Size: 825 471
BstZ17I GTA'TAC
Cuts at: 0 420 1296
Size: 420 876
BtgI C'CryG_G
Cuts at: 0 11 396 531 1296
Size: 11 385 135 765
Fragments arranged by size:
765 385 135 11
BtsI GCAGTG_nn'
Cuts at: 0 92 330 1256 1296
Size: 92 238 926 40
Fragments arranged by size:
926 238 92 40
ClaI AT'CG_AT
Cuts at: 0 263 1296
Size: 263 1033
DrdI GACnn_nn'nnGTC
Cuts at: 0 487 1296
Size: 487 809
EaeI y'GGCC_r
Cuts at: 0 44 618 1152 1296
Size: 44 574 534 144
Fragments arranged by size:
574 534 144 44
EagI C'GGCC_G
Cuts at: 0 1152 1296
Size: 1152 144
EarI CTCTTCn'nnn_
Cuts at: 0 335 1129 1262 1296
Size: 335 794 133 34
Fragments arranged by size:
794 335 133 34
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 355 1216 1296
Size: 43 312 861 80
Fragments arranged by size:
861 312 80 43
EcoNI CCTnn'n_nnAGG
Cuts at: 0 25 1296
Size: 25 1271
EcoO109I rG'GnC_Cy
Cuts at: 0 1143 1158 1203 1236 1296
Size: 1143 15 45 33 60
Fragments arranged by size:
1143 60 45 33 15
EcoRI G'AATT_C
Cuts at: 0 391 1296
Size: 391 905
HaeII r_GCGC'y
Cuts at: 0 34 1296
Size: 34 1262
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1005 1037 1296
Size: 1005 32 259
Fragments arranged by size:
1005 259 32
HincII GTy'rAC
Cuts at: 0 492 1296
Size: 492 804
HindIII A'AGCT_T
Cuts at: 0 1279 1296
Size: 1279 17
KpnI G_GTAC'C
Cuts at: 0 612 1296
Size: 612 684
MscI TGG'CCA
Cuts at: 0 620 1296
Size: 620 676
MslI CAynn'nnrTG
Cuts at: 0 561 823 872 1296
Size: 561 262 49 424
Fragments arranged by size:
561 424 262 49
MspA1I CmG'CkG
Cuts at: 0 303 388 1047 1151 1296
Size: 303 85 659 104 145
Fragments arranged by size:
659 303 145 104 85
NcoI C'CATG_G
Cuts at: 0 396 1296
Size: 396 900
NspI r_CATG'y
Cuts at: 0 278 1296
Size: 278 1018
PmlI CAC'GTG
Cuts at: 0 66 1296
Size: 66 1230
PpuMI rG'GwC_Cy
Cuts at: 0 1158 1296
Size: 1158 138
PstI C_TGCA'G
Cuts at: 0 387 793 972 1296
Size: 387 406 179 324
Fragments arranged by size:
406 387 324 179
PvuII CAG'CTG
Cuts at: 0 303 388 1047 1296
Size: 303 85 659 249
Fragments arranged by size:
659 303 249 85
SapI GCTCTTCn'nnn_
Cuts at: 0 1129 1296
Size: 1129 167
SbfI CC_TGCA'GG
Cuts at: 0 793 1296
Size: 793 503
SfcI C'TryA_G
Cuts at: 0 383 789 968 1296
Size: 383 406 179 328
Fragments arranged by size:
406 383 328 179
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1151 1296
Size: 1151 145
SphI G_CATG'C
Cuts at: 0 278 1296
Size: 278 1018
SspI AAT'ATT
Cuts at: 0 150 1296
Size: 150 1146
StyI C'CwwG_G
Cuts at: 0 396 1296
Size: 396 900
TatI w'GTAC_w
Cuts at: 0 950 1296
Size: 950 346
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 538 1296
Size: 538 758
Enzymes that do cut:
AccI Acc65I AcuI AflIII AlwNI ApoI AseI BanI
BbsI BbvCI BcgI BclI BglI Bme1580I BmgBI BmrI
BpmI Bpu10I BsaAI BsaHI BseRI BseYI BsgI BsiEI
BsmI Bsp1286I BspMI BstEII BstXI BstZ17I BtgI BtsI
ClaI DrdI EaeI EagI EarI Eco57MI EcoNI EcoO109I
EcoRI HaeII Hin4I HincII HindIII KpnI MscI MslI
MspA1I NcoI NspI PmlI PpuMI PstI PvuII SapI
SbfI SfcI SfiI SphI SspI StyI TatI XcmI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BamHI BanII BbeI BciVI BfrBI BglII BlpI
BmtI BplI BpuEI BsaI BsaBI BsaWI BsaXI BsiHKAI
BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstYI Bsu36I BtgZI CspCI
DraI DraIII EciI EcoICRI EcoRV FalI FseI FspI
FspAI HpaI KasI MfeI MluI MmeI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
ScaI SexAI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SrfI StuI SwaI TaqII TstI Tth111I XbaI
XhoI XmaI XmnI ZraI