Restriction map for
pF1KB7978
(Linear) (Six-base) MAPSORT of: KIBB7978.seq Check: 8094 from: 1 to: 1287
KIBB7978.orf
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 699 1287
Size: 699 588
AfeI AGC'GCT
Cuts at: 0 1143 1287
Size: 1143 144
AleI CACnn'nnGTG
Cuts at: 0 526 1287
Size: 526 761
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 751 783 1287
Size: 751 32 504
Fragments arranged by size:
751 504 32
AlwNI CAG_nnn'CTG
Cuts at: 0 600 1287
Size: 600 687
ApaI G_GGCC'C
Cuts at: 0 173 987 1007 1287
Size: 173 814 20 280
Fragments arranged by size:
814 280 173 20
AscI GG'CGCG_CC
Cuts at: 0 180 1287
Size: 180 1107
AvaI C'yCGr_G
Cuts at: 0 190 572 686 853 894 978 1287
Size: 190 382 114 167 41 84 309
Fragments arranged by size:
382 309 190 167 114 84 41
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 344 377 1287
Size: 344 33 910
Fragments arranged by size:
910 344 33
BanI G'GyrC_C
Cuts at: 0 587 745 1287
Size: 587 158 542
Fragments arranged by size:
587 542 158
BanII G_rGCy'C
Cuts at: 0 173 239 581 987 1007 1287
Size: 173 66 342 406 20 280
Fragments arranged by size:
406 342 280 173 66 20
BbvCI CC'TCA_GC
Cuts at: 0 916 1287
Size: 916 371
BfrBI ATG'CAT
Cuts at: 0 846 1287
Size: 846 441
BglI GCCn_nnn'nGGC
Cuts at: 0 177 585 689 1187 1287
Size: 177 408 104 498 100
Fragments arranged by size:
498 408 177 104 100
BlpI GC'TnA_GC
Cuts at: 0 148 398 1287
Size: 148 250 889
Fragments arranged by size:
889 250 148
Bme1580I G_kGCm'C
Cuts at: 0 173 901 987 1007 1287
Size: 173 728 86 20 280
Fragments arranged by size:
728 280 173 86 20
BmgBI CAC'GTC
Cuts at: 0 213 342 1287
Size: 213 129 945
Fragments arranged by size:
945 213 129
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 216 408 712 1287
Size: 216 192 304 575
Fragments arranged by size:
575 304 216 192
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 1287
Size: 622 665
Bpu10I CC'TnA_GC
Cuts at: 0 916 1243 1287
Size: 916 327 44
Fragments arranged by size:
916 327 44
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 751 781 1054 1084 1287
Size: 751 30 273 30 203
Fragments arranged by size:
751 273 203 30 30
BseYI C'CCAG_C
Cuts at: 0 679 1177 1287
Size: 679 498 110
Fragments arranged by size:
679 498 110
BsiEI CG_ry'CG
Cuts at: 0 164 419 1104 1287
Size: 164 255 685 183
Fragments arranged by size:
685 255 183 164
BsiHKAI G_wGCw'C
Cuts at: 0 239 676 1249 1287
Size: 239 437 573 38
Fragments arranged by size:
573 437 239 38
Bsp1286I G_dGCh'C
Cuts at: 0 173 239 581 676 901 987 1007 1249
Size: 173 66 342 95 225 86 20 242
Cuts at: 1249 1287
Size: 38
Fragments arranged by size:
342 242 225 173 95 86 66 38
20
BspHI T'CATG_A
Cuts at: 0 422 1169 1287
Size: 422 747 118
Fragments arranged by size:
747 422 118
BsrBI CCG'CTC
Cuts at: 0 791 1001 1106 1287
Size: 791 210 105 181
Fragments arranged by size:
791 210 181 105
BsrDI GCAATG_nn'
Cuts at: 0 384 414 1287
Size: 384 30 873
Fragments arranged by size:
873 384 30
BsrFI r'CCGG_y
Cuts at: 0 590 933 1081 1159 1287
Size: 590 343 148 78 128
Fragments arranged by size:
590 343 148 128 78
BssHII G'CGCG_C
Cuts at: 0 143 152 180 1287
Size: 143 9 28 1107
Fragments arranged by size:
1107 143 28 9
BssSI C'ACGA_G
Cuts at: 0 238 900 1287
Size: 238 662 387
Fragments arranged by size:
662 387 238
BstEII G'GTnAC_C
Cuts at: 0 555 1287
Size: 555 732
BstXI CCAn_nnnn'nTGG
Cuts at: 0 528 1198 1287
Size: 528 670 89
Fragments arranged by size:
670 528 89
BtgI C'CryG_G
Cuts at: 0 164 560 1073 1235 1287
Size: 164 396 513 162 52
Fragments arranged by size:
513 396 164 162 52
BtsI GCAGTG_nn'
Cuts at: 0 477 1098 1287
Size: 477 621 189
Fragments arranged by size:
621 477 189
EaeI y'GGCC_r
Cuts at: 0 161 416 437 935 1079 1188 1287
Size: 161 255 21 498 144 109 99
Fragments arranged by size:
498 255 161 144 109 99 21
EagI C'GGCC_G
Cuts at: 0 161 416 1287
Size: 161 255 871
Fragments arranged by size:
871 255 161
EarI CTCTTCn'nnn_
Cuts at: 0 478 1287
Size: 478 809
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 235 1287
Size: 235 1052
EcoICRI GAG'CTC
Cuts at: 0 237 1287
Size: 237 1050
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 699 1287
Size: 622 77 588
Fragments arranged by size:
622 588 77
EcoO109I rG'GnC_Cy
Cuts at: 0 169 639 983 984 1004 1287
Size: 169 470 344 1 20 283
Fragments arranged by size:
470 344 283 169 20 1
FspI TGC'GCA
Cuts at: 0 244 406 655 1287
Size: 244 162 249 632
Fragments arranged by size:
632 249 244 162
FspAI rTGC'GCAy
Cuts at: 0 244 1287
Size: 244 1043
HaeII r_GCGC'y
Cuts at: 0 294 887 1145 1287
Size: 294 593 258 142
Fragments arranged by size:
593 294 258 142
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 834 866 1287
Size: 834 32 421
Fragments arranged by size:
834 421 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 794 1287
Size: 794 493
MscI TGG'CCA
Cuts at: 0 439 1287
Size: 439 848
MslI CAynn'nnrTG
Cuts at: 0 505 526 553 661 1287
Size: 505 21 27 108 626
Fragments arranged by size:
626 505 108 27 21
MspA1I CmG'CkG
Cuts at: 0 140 397 868 1287
Size: 140 257 471 419
Fragments arranged by size:
471 419 257 140
NaeI GCC'GGC
Cuts at: 0 935 1083 1161 1287
Size: 935 148 78 126
Fragments arranged by size:
935 148 126 78
NcoI C'CATG_G
Cuts at: 0 1073 1287
Size: 1073 214
NgoMIV G'CCGG_C
Cuts at: 0 933 1081 1159 1287
Size: 933 148 78 128
Fragments arranged by size:
933 148 128 78
NsiI A_TGCA'T
Cuts at: 0 848 1287
Size: 848 439
NspI r_CATG'y
Cuts at: 0 660 933 1122 1287
Size: 660 273 189 165
Fragments arranged by size:
660 273 189 165
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 751 783 1287
Size: 751 32 504
Fragments arranged by size:
751 504 32
PpuMI rG'GwC_Cy
Cuts at: 0 639 1287
Size: 639 648
PspOMI G'GGCC_C
Cuts at: 0 169 983 1003 1287
Size: 169 814 20 284
Fragments arranged by size:
814 284 169 20
PvuII CAG'CTG
Cuts at: 0 868 1287
Size: 868 419
SacI G_AGCT'C
Cuts at: 0 239 1287
Size: 239 1048
SexAI A'CCwGG_T
Cuts at: 0 431 1287
Size: 431 856
SfcI C'TryA_G
Cuts at: 0 879 1287
Size: 879 408
SmaI CCC'GGG
Cuts at: 0 574 855 980 1287
Size: 574 281 125 307
Fragments arranged by size:
574 307 281 125
SphI G_CATG'C
Cuts at: 0 660 933 1122 1287
Size: 660 273 189 165
Fragments arranged by size:
660 273 189 165
StyI C'CwwG_G
Cuts at: 0 741 1073 1287
Size: 741 332 214
Fragments arranged by size:
741 332 214
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 538 693 1248 1287
Size: 538 155 555 39
Fragments arranged by size:
555 538 155 39
XmaI C'CCGG_G
Cuts at: 0 572 853 978 1287
Size: 572 281 125 309
Fragments arranged by size:
572 309 281 125
XmnI GAAnn'nnTTC
Cuts at: 0 487 763 1287
Size: 487 276 524
Fragments arranged by size:
524 487 276
Enzymes that do cut:
AcuI AfeI AleI AloI AlwNI ApaI AscI AvaI
BaeI BanI BanII BbvCI BfrBI BglI BlpI Bme1580I
BmgBI BplI BpmI Bpu10I BsaXI BseYI BsiEI BsiHKAI
Bsp1286I BspHI BsrBI BsrDI BsrFI BssHII BssSI BstEII
BstXI BtgI BtsI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I FspI FspAI HaeII Hin4I MmeI MscI
MslI MspA1I NaeI NcoI NgoMIV NsiI NspI PpiI
PpuMI PspOMI PvuII SacI SexAI SfcI SmaI SphI
StyI TaqII XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AlfI ApaLI ApoI AseI AsiSI AvrII BamHI
BbeI BbsI BcgI BciVI BclI BglII BmrI BmtI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BsgI
BsiWI BsmI BsmBI BspEI BspMI BsrGI BstAPI BstBI
BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EcoNI EcoRI EcoRV FalI FseI HincII HindIII
HpaI KasI KpnI MfeI MluI NarI NdeI NheI
NotI NruI PacI PasI PciI PflMI PfoI PmeI
PmlI PshAI PsiI PspXI PsrI PstI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SrfI SspI StuI
SwaI TatI TstI Tth111I XbaI XcmI XhoI ZraI