Restriction map for
pF1KB7972
(Linear) (Six-base) MAPSORT of: KIBB7972.seq Check: 746 from: 1 to: 1233
KIBB7972.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 0 1078 1233
Size: 1078 155
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 1233
Size: 26 1207
AflIII A'CryG_T
Cuts at: 0 28 954 1233
Size: 28 926 279
Fragments arranged by size:
926 279 28
ApoI r'AATT_y
Cuts at: 0 507 688 1192 1233
Size: 507 181 504 41
Fragments arranged by size:
507 504 181 41
AvaI C'yCGr_G
Cuts at: 0 103 1233
Size: 103 1130
BamHI G'GATC_C
Cuts at: 0 348 1233
Size: 348 885
BanI G'GyrC_C
Cuts at: 0 133 1233
Size: 133 1100
BbeI G_GCGC'C
Cuts at: 0 137 1233
Size: 137 1096
BbvCI CC'TCA_GC
Cuts at: 0 187 298 1233
Size: 187 111 935
Fragments arranged by size:
935 187 111
BciVI GTATCCnnnnn_n'
Cuts at: 0 1028 1233
Size: 1028 205
BglI GCCn_nnn'nGGC
Cuts at: 0 20 256 1233
Size: 20 236 977
Fragments arranged by size:
977 236 20
BlpI GC'TnA_GC
Cuts at: 0 293 1233
Size: 293 940
BmrI ACTGGGnnnn_n'
Cuts at: 0 52 922 1195 1233
Size: 52 870 273 38
Fragments arranged by size:
870 273 52 38
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 658 1233
Size: 301 357 575
Fragments arranged by size:
575 357 301
Bpu10I CC'TnA_GC
Cuts at: 0 187 298 716 1233
Size: 187 111 418 517
Fragments arranged by size:
517 418 187 111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 1233
Size: 355 878
BsaAI yAC'GTr
Cuts at: 0 29 1233
Size: 29 1204
BsaHI Gr'CG_yC
Cuts at: 0 134 1233
Size: 134 1099
BsaWI w'CCGG_w
Cuts at: 0 363 1233
Size: 363 870
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 46 76 1233
Size: 46 30 1157
Fragments arranged by size:
1157 46 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1144 1233
Size: 1144 89
BsiEI CG_ry'CG
Cuts at: 0 240 1233
Size: 240 993
BsiHKAI G_wGCw'C
Cuts at: 0 722 1233
Size: 722 511
BsmI GAATG_Cn'
Cuts at: 0 802 1160 1233
Size: 802 358 73
Fragments arranged by size:
802 358 73
Bsp1286I G_dGCh'C
Cuts at: 0 722 1233
Size: 722 511
BspEI T'CCGG_A
Cuts at: 0 363 1233
Size: 363 870
BspHI T'CATG_A
Cuts at: 0 990 1233
Size: 990 243
BsrBI CCG'CTC
Cuts at: 0 208 1233
Size: 208 1025
BsrFI r'CCGG_y
Cuts at: 0 198 1233
Size: 198 1035
BssHII G'CGCG_C
Cuts at: 0 117 1233
Size: 117 1116
BstXI CCAn_nnnn'nTGG
Cuts at: 0 267 682 1233
Size: 267 415 551
Fragments arranged by size:
551 415 267
BstYI r'GATC_y
Cuts at: 0 264 348 1233
Size: 264 84 885
Fragments arranged by size:
885 264 84
BstZ17I GTA'TAC
Cuts at: 0 1079 1233
Size: 1079 154
BtgI C'CryG_G
Cuts at: 0 251 1233
Size: 251 982
BtsI GCAGTG_nn'
Cuts at: 0 75 1233
Size: 75 1158
EaeI y'GGCC_r
Cuts at: 0 257 1233
Size: 257 976
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 301 658 1233
Size: 26 275 357 575
Fragments arranged by size:
575 357 275 26
EcoO109I rG'GnC_Cy
Cuts at: 0 54 1122 1233
Size: 54 1068 111
Fragments arranged by size:
1068 111 54
EcoRI G'AATT_C
Cuts at: 0 688 1192 1233
Size: 688 504 41
Fragments arranged by size:
688 504 41
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 553 1233
Size: 553 680
HaeII r_GCGC'y
Cuts at: 0 137 1233
Size: 137 1096
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 46 78 463 495 1002 1034 1233
Size: 46 32 385 32 507 32 199
Fragments arranged by size:
507 385 199 46 32 32 32
KasI G'GCGC_C
Cuts at: 0 133 1233
Size: 133 1100
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 1233
Size: 260 973
MscI TGG'CCA
Cuts at: 0 259 1233
Size: 259 974
MslI CAynn'nnrTG
Cuts at: 0 992 1233
Size: 992 241
MspA1I CmG'CkG
Cuts at: 0 229 253 1233
Size: 229 24 980
Fragments arranged by size:
980 229 24
NaeI GCC'GGC
Cuts at: 0 200 1233
Size: 200 1033
NarI GG'CG_CC
Cuts at: 0 134 1233
Size: 134 1099
NgoMIV G'CCGG_C
Cuts at: 0 198 1233
Size: 198 1035
NspI r_CATG'y
Cuts at: 0 487 958 1233
Size: 487 471 275
Fragments arranged by size:
487 471 275
PciI A'CATG_T
Cuts at: 0 954 1233
Size: 954 279
PfoI T'CCnGG_A
Cuts at: 0 1218 1233
Size: 1218 15
PpuMI rG'GwC_Cy
Cuts at: 0 54 1122 1233
Size: 54 1068 111
Fragments arranged by size:
1068 111 54
PsiI TTA'TAA
Cuts at: 0 776 1233
Size: 776 457
PstI C_TGCA'G
Cuts at: 0 277 451 1233
Size: 277 174 782
Fragments arranged by size:
782 277 174
PvuII CAG'CTG
Cuts at: 0 229 1233
Size: 229 1004
SacII CC_GC'GG
Cuts at: 0 254 1233
Size: 254 979
SexAI A'CCwGG_T
Cuts at: 0 377 1233
Size: 377 856
SfcI C'TryA_G
Cuts at: 0 273 447 1233
Size: 273 174 786
Fragments arranged by size:
786 273 174
SfoI GGC'GCC
Cuts at: 0 135 1233
Size: 135 1098
SmlI C'TyrA_G
Cuts at: 0 334 1233
Size: 334 899
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 31 1080 1233
Size: 31 1049 153
Fragments arranged by size:
1049 153 31
Tth111I GACn'n_nGTC
Cuts at: 0 58 1233
Size: 58 1175
XmnI GAAnn'nnTTC
Cuts at: 0 359 1233
Size: 359 874
Enzymes that do cut:
AccI AcuI AflIII ApoI AvaI BamHI BanI BbeI
BbvCI BciVI BglI BlpI BmrI BpmI Bpu10I BpuEI
BsaAI BsaHI BsaWI BsaXI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BsrBI BsrFI BssHII BstXI BstYI
BstZ17I BtgI BtsI EaeI Eco57MI EcoO109I EcoRI FalI
HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PfoI PpuMI PsiI PstI
PvuII SacII SexAI SfcI SfoI SmlI TaqII Tth111I
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BanII BbsI BcgI BclI BfrBI
BglII Bme1580I BmgBI BmtI BplI BsaI BsaBI BseRI
BseYI BsiWI BsmBI BspMI BsrDI BsrGI BssSI BstAPI
BstBI BstEII Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EarI EciI EcoICRI EcoNI EcoRV FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI
PvuI RsrII SacI SalI SanDI SapI SbfI ScaI
SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TatI TstI XbaI XcmI
XhoI XmaI ZraI