Restriction map for
pF1KB7971
(Linear) (Six-base) MAPSORT of: KIBB7971.seq Check: 2057 from: 1 to: 870
KIBB7971.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 0 144 870
Size: 144 726
AleI CACnn'nnGTG
Cuts at: 0 652 870
Size: 652 218
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 734 766 870
Size: 734 32 104
Fragments arranged by size:
734 104 32
AlwNI CAG_nnn'CTG
Cuts at: 0 399 448 870
Size: 399 49 422
Fragments arranged by size:
422 399 49
ApaI G_GGCC'C
Cuts at: 0 614 870
Size: 614 256
ApoI r'AATT_y
Cuts at: 0 130 870
Size: 130 740
AvaI C'yCGr_G
Cuts at: 0 91 870
Size: 91 779
BamHI G'GATC_C
Cuts at: 0 108 693 870
Size: 108 585 177
Fragments arranged by size:
585 177 108
BanII G_rGCy'C
Cuts at: 0 367 614 870
Size: 367 247 256
Fragments arranged by size:
367 256 247
BbsI GAAGACnn'nnnn_
Cuts at: 0 233 870
Size: 233 637
BciVI GTATCCnnnnn_n'
Cuts at: 0 201 870
Size: 201 669
BclI T'GATC_A
Cuts at: 0 162 870
Size: 162 708
BlpI GC'TnA_GC
Cuts at: 0 34 870
Size: 34 836
Bme1580I G_kGCm'C
Cuts at: 0 614 870
Size: 614 256
BmrI ACTGGGnnnn_n'
Cuts at: 0 419 870
Size: 419 451
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 479 614 870
Size: 479 135 256
Fragments arranged by size:
479 256 135
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 779 870
Size: 779 91
BsaWI w'CCGG_w
Cuts at: 0 71 870
Size: 71 799
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 53 83 373 403 870
Size: 53 30 290 30 467
Fragments arranged by size:
467 290 53 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 337 375 870
Size: 337 38 495
Fragments arranged by size:
495 337 38
BseYI C'CCAG_C
Cuts at: 0 724 870
Size: 724 146
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 870
Size: 283 587
BsiHKAI G_wGCw'C
Cuts at: 0 35 367 870
Size: 35 332 503
Fragments arranged by size:
503 332 35
BsmI GAATG_Cn'
Cuts at: 0 293 870
Size: 293 577
Bsp1286I G_dGCh'C
Cuts at: 0 35 367 614 870
Size: 35 332 247 256
Fragments arranged by size:
332 256 247 35
BspEI T'CCGG_A
Cuts at: 0 71 870
Size: 71 799
BstYI r'GATC_y
Cuts at: 0 108 693 870
Size: 108 585 177
Fragments arranged by size:
585 177 108
BtgI C'CryG_G
Cuts at: 0 333 870
Size: 333 537
BtsI GCAGTG_nn'
Cuts at: 0 277 659 870
Size: 277 382 211
Fragments arranged by size:
382 277 211
DraI TTT'AAA
Cuts at: 0 135 870
Size: 135 735
EarI CTCTTCn'nnn_
Cuts at: 0 779 870
Size: 779 91
EcoICRI GAG'CTC
Cuts at: 0 365 870
Size: 365 505
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 479 614 870
Size: 479 135 256
Fragments arranged by size:
479 256 135
EcoO109I rG'GnC_Cy
Cuts at: 0 5 870
Size: 5 865
EcoRV GAT'ATC
Cuts at: 0 590 870
Size: 590 280
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 820 870
Size: 820 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 53 85 870
Size: 53 32 785
Fragments arranged by size:
785 53 32
HindIII A'AGCT_T
Cuts at: 0 573 870
Size: 573 297
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 39 777 870
Size: 29 10 738 93
Fragments arranged by size:
738 93 29 10
MslI CAynn'nnrTG
Cuts at: 0 652 870
Size: 652 218
NcoI C'CATG_G
Cuts at: 0 333 870
Size: 333 537
PasI CC'CwG_GG
Cuts at: 0 468 870
Size: 468 402
PflMI CCAn_nnn'nTGG
Cuts at: 0 334 848 870
Size: 334 514 22
Fragments arranged by size:
514 334 22
PfoI T'CCnGG_A
Cuts at: 0 200 870
Size: 200 670
PpuMI rG'GwC_Cy
Cuts at: 0 5 870
Size: 5 865
PspOMI G'GGCC_C
Cuts at: 0 610 870
Size: 610 260
SacI G_AGCT'C
Cuts at: 0 367 870
Size: 367 503
SmlI C'TyrA_G
Cuts at: 0 794 870
Size: 794 76
StyI C'CwwG_G
Cuts at: 0 333 482 870
Size: 333 149 388
Fragments arranged by size:
388 333 149
Tth111I GACn'n_nGTC
Cuts at: 0 673 870
Size: 673 197
Enzymes that do cut:
AccI AleI AloI AlwNI ApaI ApoI AvaI BamHI
BanII BbsI BciVI BclI BlpI Bme1580I BmrI BpmI
BpuEI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspEI BstYI BtgI BtsI DraI EarI EcoICRI
Eco57MI EcoO109I EcoRV FalI Hin4I HindIII MmeI MslI
NcoI PasI PflMI PfoI PpuMI PspOMI SacI SmlI
StyI Tth111I
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI ApaLI AscI AseI AsiSI AvrII
BaeI BanI BbeI BbvCI BcgI BfrBI BglI BglII
BmgBI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaHI
BsiEI BsiWI BsmBI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI DraIII DrdI EaeI EagI
EciI EcoNI EcoRI FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MfeI MluI MscI MspA1I NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PstI PvuI PvuII RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TatI TstI XbaI XcmI XhoI XmaI
XmnI ZraI