Restriction map for
pF1KB7959
(Linear) (Six-base) MAPSORT of: KIBB7959.seq Check: 6280 from: 1 to: 792
KIBB7959.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AflIII A'CryG_T
Cuts at: 0 127 792
Size: 127 665
AleI CACnn'nnGTG
Cuts at: 0 119 752 792
Size: 119 633 40
Fragments arranged by size:
633 119 40
AlwNI CAG_nnn'CTG
Cuts at: 0 306 477 612 792
Size: 306 171 135 180
Fragments arranged by size:
306 180 171 135
ApoI r'AATT_y
Cuts at: 0 256 295 792
Size: 256 39 497
Fragments arranged by size:
497 256 39
AvaI C'yCGr_G
Cuts at: 0 550 792
Size: 550 242
BanI G'GyrC_C
Cuts at: 0 679 792
Size: 679 113
BanII G_rGCy'C
Cuts at: 0 232 792
Size: 232 560
BbsI GAAGACnn'nnnn_
Cuts at: 0 740 792
Size: 740 52
BglI GCCn_nnn'nGGC
Cuts at: 0 218 792
Size: 218 574
BlpI GC'TnA_GC
Cuts at: 0 73 792
Size: 73 719
Bme1580I G_kGCm'C
Cuts at: 0 471 749 792
Size: 471 278 43
Fragments arranged by size:
471 278 43
BmgBI CAC'GTC
Cuts at: 0 117 792
Size: 117 675
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 576 792
Size: 576 216
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 792
Size: 313 479
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 563 593 792
Size: 563 30 199
Fragments arranged by size:
563 199 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 222 679 792
Size: 222 457 113
Fragments arranged by size:
457 222 113
BseYI C'CCAG_C
Cuts at: 0 90 245 599 705 792
Size: 90 155 354 106 87
Fragments arranged by size:
354 155 106 90 87
BsiEI CG_ry'CG
Cuts at: 0 218 282 792
Size: 218 64 510
Fragments arranged by size:
510 218 64
BsiHKAI G_wGCw'C
Cuts at: 0 116 500 792
Size: 116 384 292
Fragments arranged by size:
384 292 116
Bsp1286I G_dGCh'C
Cuts at: 0 116 232 471 500 749 792
Size: 116 116 239 29 249 43
Fragments arranged by size:
249 239 116 116 43 29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 498 528 792
Size: 498 30 264
Fragments arranged by size:
498 264 30
BsrBI CCG'CTC
Cuts at: 0 547 792
Size: 547 245
BsrFI r'CCGG_y
Cuts at: 0 153 217 663 682 792
Size: 153 64 446 19 110
Fragments arranged by size:
446 153 110 64 19
BstEII G'GTnAC_C
Cuts at: 0 540 792
Size: 540 252
Bsu36I CC'TnA_GG
Cuts at: 0 23 792
Size: 23 769
BtgI C'CryG_G
Cuts at: 0 120 638 792
Size: 120 518 154
Fragments arranged by size:
518 154 120
DraIII CAC_nnn'GTG
Cuts at: 0 384 792
Size: 384 408
EaeI y'GGCC_r
Cuts at: 0 215 350 792
Size: 215 135 442
Fragments arranged by size:
442 215 135
EagI C'GGCC_G
Cuts at: 0 215 792
Size: 215 577
EcoNI CCTnn'n_nnAGG
Cuts at: 0 26 727 792
Size: 26 701 65
Fragments arranged by size:
701 65 26
EcoO109I rG'GnC_Cy
Cuts at: 0 179 474 609 792
Size: 179 295 135 183
Fragments arranged by size:
295 183 179 135
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 33 792
Size: 33 759
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 89 121 360 392 563 595 792
Size: 89 32 239 32 171 32 197
Fragments arranged by size:
239 197 171 89 32 32 32
MluI A'CGCG_T
Cuts at: 0 127 792
Size: 127 665
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 594 767 792
Size: 594 173 25
Fragments arranged by size:
594 173 25
MscI TGG'CCA
Cuts at: 0 352 792
Size: 352 440
MslI CAynn'nnrTG
Cuts at: 0 11 119 421 752 792
Size: 11 108 302 331 40
Fragments arranged by size:
331 302 108 40 11
MspA1I CmG'CkG
Cuts at: 0 43 594 603 792
Size: 43 551 9 189
Fragments arranged by size:
551 189 43 9
NaeI GCC'GGC
Cuts at: 0 155 219 665 792
Size: 155 64 446 127
Fragments arranged by size:
446 155 127 64
NgoMIV G'CCGG_C
Cuts at: 0 153 217 663 792
Size: 153 64 446 129
Fragments arranged by size:
446 153 129 64
PpuMI rG'GwC_Cy
Cuts at: 0 179 474 792
Size: 179 295 318
Fragments arranged by size:
318 295 179
PstI C_TGCA'G
Cuts at: 0 56 608 650 792
Size: 56 552 42 142
Fragments arranged by size:
552 142 56 42
PvuII CAG'CTG
Cuts at: 0 594 603 792
Size: 594 9 189
Fragments arranged by size:
594 189 9
SanDI GG'GwC_CC
Cuts at: 0 179 792
Size: 179 613
SfcI C'TryA_G
Cuts at: 0 52 604 646 792
Size: 52 552 42 146
Fragments arranged by size:
552 146 52 42
SmlI C'TyrA_G
Cuts at: 0 328 792
Size: 328 464
StyI C'CwwG_G
Cuts at: 0 101 728 792
Size: 101 627 64
Fragments arranged by size:
627 101 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 775 792
Size: 775 17
TatI w'GTAC_w
Cuts at: 0 389 792
Size: 389 403
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 580 612 792
Size: 580 32 180
Fragments arranged by size:
580 180 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 772 792
Size: 772 20
XmnI GAAnn'nnTTC
Cuts at: 0 16 792
Size: 16 776
Enzymes that do cut:
AflIII AleI AlwNI ApoI AvaI BanI BanII BbsI
BglI BlpI Bme1580I BmgBI BplI BpuEI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BstEII
Bsu36I BtgI DraIII EaeI EagI EcoNI EcoO109I FalI
Hin4I MluI MmeI MscI MslI MspA1I NaeI NgoMIV
PpuMI PstI PvuII SanDI SfcI SmlI StyI TaqII
TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BfrBI BglII BmrI BmtI BpmI Bpu10I BsaI
BsaAI BsaBI BsaHI BsaWI BsgI BsiWI BsmI BsmBI
BspEI BspHI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstXI BstYI BstZ17I BtgZI BtsI ClaI CspCI DraI
DrdI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI NarI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI Tth111I XbaI XhoI XmaI ZraI