(Linear) (Six-base) MAPSORT of: KIBB7955.seq  Check: 2014  from: 1  to: 768



KIBB7955.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:25  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     195     768 
   Size:        195     573
 
AflIII A'CryG_T

Cuts at:      0      45     768 
   Size:         45     723
 
AleI CACnn'nnGTG

Cuts at:      0     316     768 
   Size:        316     452
 
ApoI r'AATT_y

Cuts at:      0     123     361     768 
   Size:        123     238     407

  Fragments arranged by size: 

                 407     238     123
 
AvaI C'yCGr_G

Cuts at:      0     506     768 
   Size:        506     262
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     212     245     768 
   Size:        212      33     523

  Fragments arranged by size: 

                 523     212      33
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     604     768 
   Size:        604     164
 
Bpu10I CC'TnA_GC

Cuts at:      0     253     768 
   Size:        253     515
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     559     768 
   Size:        559     209
 
BsaWI w'CCGG_w

Cuts at:      0     702     768 
   Size:        702      66
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0       8      38     768 
   Size:          8      30     730

  Fragments arranged by size: 

                 730      30       8
 
BseYI C'CCAG_C

Cuts at:      0     197     687     768 
   Size:        197     490      81

  Fragments arranged by size: 

                 490     197      81
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     195     768 
   Size:        195     573
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     695     768 
   Size:        695      73
 
Bsu36I CC'TnA_GG

Cuts at:      0      88     768 
   Size:         88     680
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     456     768 
   Size:        456     312
 
BtsI GCAGTG_nn'

Cuts at:      0      77     768 
   Size:         77     691
 
DraIII CAC_nnn'GTG

Cuts at:      0     372     768 
   Size:        372     396
 
EarI CTCTTCn'nnn_

Cuts at:      0     562     768 
   Size:        562     206
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       8      40     768 
   Size:          8      32     728

  Fragments arranged by size: 

                 728      32       8
 
HincII GTy'rAC

Cuts at:      0     600     768 
   Size:        600     168
 
MslI CAynn'nnrTG

Cuts at:      0      50     316     768 
   Size:         50     266     452

  Fragments arranged by size: 

                 452     266      50
 
MspA1I CmG'CkG

Cuts at:      0     733     768 
   Size:        733      35
 
NspI r_CATG'y

Cuts at:      0      49     104     205     768 
   Size:         49      55     101     563

  Fragments arranged by size: 

                 563     101      55      49
 
PciI A'CATG_T

Cuts at:      0      45     768 
   Size:         45     723
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      62     768 
   Size:         62     706
 
PstI C_TGCA'G

Cuts at:      0      84     768 
   Size:         84     684
 
PvuII CAG'CTG

Cuts at:      0     733     768 
   Size:        733      35
 
ScaI AGT'ACT

Cuts at:      0     748     768 
   Size:        748      20
 
SfcI C'TryA_G

Cuts at:      0      11      80     326     768 
   Size:         11      69     246     442

  Fragments arranged by size: 

                 442     246      69      11
 
SphI G_CATG'C

Cuts at:      0     205     768 
   Size:        205     563
 
SspI AAT'ATT

Cuts at:      0     280     768 
   Size:        280     488
 
StuI AGG'CCT

Cuts at:      0      93     768 
   Size:         93     675
 
StyI C'CwwG_G

Cuts at:      0      61     768 
   Size:         61     707
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     211     768 
   Size:        211     557
 
TatI w'GTAC_w

Cuts at:      0     746     768 
   Size:        746      22
 
XmnI GAAnn'nnTTC

Cuts at:      0     652     768 
   Size:        652     116
 

 Enzymes that do cut:

     AarI   AflIII     AleI     ApoI     AvaI     BaeI     BmrI   Bpu10I
     BsaI    BsaWI    BsaXI    BseYI    BspMI    BstXI   Bsu36I    BtgZI
     BtsI   DraIII     EarI    Hin4I   HincII     MslI   MspA1I     NspI
     PciI    PflMI     PstI    PvuII     ScaI     SfcI     SphI     SspI
     StuI     StyI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BplI     BpmI    BpuEI    BsaAI    BsaBI    BsaHI
    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI  BstZ17I     BtgI     ClaI    CspCI
     DraI     DrdI     EaeI     EagI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI     SwaI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI