Restriction map for
pF1KB7954
(Linear) (Six-base) MAPSORT of: KIBB7954.seq Check: 8123 from: 1 to: 1185
KIBB7954.orf
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 578 704 713 968 1033 1147 1185
Size: 578 126 9 255 65 114 38
Fragments arranged by size:
578 255 126 114 65 38 9
AflII C'TTAA_G
Cuts at: 0 271 1185
Size: 271 914
AflIII A'CryG_T
Cuts at: 0 302 1185
Size: 302 883
AlwNI CAG_nnn'CTG
Cuts at: 0 238 766 1185
Size: 238 528 419
Fragments arranged by size:
528 419 238
ApoI r'AATT_y
Cuts at: 0 403 600 836 849 1185
Size: 403 197 236 13 336
Fragments arranged by size:
403 336 236 197 13
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 614 647 1185
Size: 614 33 538
Fragments arranged by size:
614 538 33
BanI G'GyrC_C
Cuts at: 0 775 1185
Size: 775 410
BanII G_rGCy'C
Cuts at: 0 90 1185
Size: 90 1095
BbsI GAAGACnn'nnnn_
Cuts at: 0 1153 1185
Size: 1153 32
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1061 1185
Size: 1061 124
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 581 713 1185
Size: 581 132 472
Fragments arranged by size:
581 472 132
Bpu10I CC'TnA_GC
Cuts at: 0 687 1185
Size: 687 498
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 554 584 1085 1115 1185
Size: 554 30 501 30 70
Fragments arranged by size:
554 501 70 30 30
BseYI C'CCAG_C
Cuts at: 0 108 1185
Size: 108 1077
BsiHKAI G_wGCw'C
Cuts at: 0 90 1185
Size: 90 1095
Bsp1286I G_dGCh'C
Cuts at: 0 90 1185
Size: 90 1095
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 37 1185
Size: 37 1148
BsrDI GCAATG_nn'
Cuts at: 0 941 1185
Size: 941 244
BsrGI T'GTAC_A
Cuts at: 0 387 1185
Size: 387 798
BssSI C'ACGA_G
Cuts at: 0 41 1185
Size: 41 1144
EaeI y'GGCC_r
Cuts at: 0 737 1185
Size: 737 448
EarI CTCTTCn'nnn_
Cuts at: 0 660 1028 1052 1185
Size: 660 368 24 133
Fragments arranged by size:
660 368 133 24
EcoICRI GAG'CTC
Cuts at: 0 88 1185
Size: 88 1097
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 578 581 704 713 968 1033 1147 1185
Size: 578 3 123 9 255 65 114 38
Fragments arranged by size:
578 255 123 114 65 38 9 3
EcoRI G'AATT_C
Cuts at: 0 836 1185
Size: 836 349
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 303 335 977 1009 1085 1117 1185
Size: 303 32 642 32 76 32 68
Fragments arranged by size:
642 303 76 68 32 32 32
HindIII A'AGCT_T
Cuts at: 0 25 1185
Size: 25 1160
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 889 1124 1185
Size: 889 235 61
Fragments arranged by size:
889 235 61
MscI TGG'CCA
Cuts at: 0 739 1185
Size: 739 446
MslI CAynn'nnrTG
Cuts at: 0 892 1185
Size: 892 293
MspA1I CmG'CkG
Cuts at: 0 1059 1185
Size: 1059 126
NdeI CA'TA_TG
Cuts at: 0 227 1185
Size: 227 958
NspI r_CATG'y
Cuts at: 0 306 532 1185
Size: 306 226 653
Fragments arranged by size:
653 306 226
PciI A'CATG_T
Cuts at: 0 302 1185
Size: 302 883
PflMI CCAn_nnn'nTGG
Cuts at: 0 871 1185
Size: 871 314
PsiI TTA'TAA
Cuts at: 0 961 1185
Size: 961 224
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 623 655 1185
Size: 623 32 530
Fragments arranged by size:
623 530 32
PstI C_TGCA'G
Cuts at: 0 762 1026 1185
Size: 762 264 159
Fragments arranged by size:
762 264 159
PvuII CAG'CTG
Cuts at: 0 1059 1185
Size: 1059 126
SacI G_AGCT'C
Cuts at: 0 90 1185
Size: 90 1095
SapI GCTCTTCn'nnn_
Cuts at: 0 1052 1185
Size: 1052 133
SfcI C'TryA_G
Cuts at: 0 98 758 1022 1131 1185
Size: 98 660 264 109 54
Fragments arranged by size:
660 264 109 98 54
SmlI C'TyrA_G
Cuts at: 0 271 1185
Size: 271 914
StuI AGG'CCT
Cuts at: 0 1129 1185
Size: 1129 56
StyI C'CwwG_G
Cuts at: 0 608 1185
Size: 608 577
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 227 1185
Size: 227 958
TatI w'GTAC_w
Cuts at: 0 387 1185
Size: 387 798
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 554 586 1185
Size: 554 32 599
Fragments arranged by size:
599 554 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 204 1185
Size: 204 981
XmnI GAAnn'nnTTC
Cuts at: 0 654 1046 1185
Size: 654 392 139
Fragments arranged by size:
654 392 139
Enzymes that do cut:
AcuI AflII AflIII AlwNI ApoI BaeI BanI BanII
BbsI BplI BpmI Bpu10I BsaXI BseYI BsiHKAI Bsp1286I
BspMI BsrDI BsrGI BssSI EaeI EarI EcoICRI Eco57MI
EcoRI Hin4I HindIII MmeI MscI MslI MspA1I NdeI
NspI PciI PflMI PsiI PsrI PstI PvuII SacI
SapI SfcI SmlI StuI StyI TaqII TatI TstI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BamHI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BsgI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI
BssHII BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoNI EcoO109I EcoRV FalI FseI FspI
FspAI HaeII HincII HpaI KasI KpnI MfeI MluI
NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI
PacI PasI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PspXI PvuI RsrII SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI
XmaI ZraI