Restriction map for
pF1KB7950
(Linear) (Six-base) MAPSORT of: KIBB7950.seq Check: 5414 from: 1 to: 1182
KIBB7950.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 758 1182
Size: 758 424
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 746 1182
Size: 746 436
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 334 366 1182
Size: 334 32 816
Fragments arranged by size:
816 334 32
AlwNI CAG_nnn'CTG
Cuts at: 0 630 1182
Size: 630 552
ApaLI G'TGCA_C
Cuts at: 0 291 1182
Size: 291 891
ApoI r'AATT_y
Cuts at: 0 184 1182
Size: 184 998
AscI GG'CGCG_CC
Cuts at: 0 437 1182
Size: 437 745
AvaI C'yCGr_G
Cuts at: 0 30 256 314 392 443 1182
Size: 30 226 58 78 51 739
Fragments arranged by size:
739 226 78 58 51 30
BamHI G'GATC_C
Cuts at: 0 678 1182
Size: 678 504
BanI G'GyrC_C
Cuts at: 0 159 195 510 1011 1182
Size: 159 36 315 501 171
Fragments arranged by size:
501 315 171 159 36
BanII G_rGCy'C
Cuts at: 0 574 1182
Size: 574 608
BbeI G_GCGC'C
Cuts at: 0 163 514 1182
Size: 163 351 668
Fragments arranged by size:
668 351 163
BbvCI CC'TCA_GC
Cuts at: 0 1000 1182
Size: 1000 182
Bme1580I G_kGCm'C
Cuts at: 0 295 1016 1182
Size: 295 721 166
Fragments arranged by size:
721 295 166
BmgBI CAC'GTC
Cuts at: 0 212 1182
Size: 212 970
BmrI ACTGGGnnnn_n'
Cuts at: 0 879 1182
Size: 879 303
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 644 1182
Size: 644 538
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 93 424 731 823 976 1182
Size: 40 53 331 307 92 153 206
Fragments arranged by size:
331 307 206 153 92 53 40
Bpu10I CC'TnA_GC
Cuts at: 0 1000 1057 1182
Size: 1000 57 125
Fragments arranged by size:
1000 125 57
BsaHI Gr'CG_yC
Cuts at: 0 160 463 511 1182
Size: 160 303 48 671
Fragments arranged by size:
671 303 160 48
BsaWI w'CCGG_w
Cuts at: 0 295 1182
Size: 295 887
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 334 364 1182
Size: 334 30 818
Fragments arranged by size:
818 334 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 850 1182
Size: 850 332
BseYI C'CCAG_C
Cuts at: 0 163 332 487 531 1182
Size: 163 169 155 44 651
Fragments arranged by size:
651 169 163 155 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 465 1182
Size: 465 717
BsiEI CG_ry'CG
Cuts at: 0 423 1182
Size: 423 759
BsiHKAI G_wGCw'C
Cuts at: 0 295 1182
Size: 295 887
BsmBI CGTCTCn'nnnn_
Cuts at: 0 293 1182
Size: 293 889
Bsp1286I G_dGCh'C
Cuts at: 0 295 574 1016 1182
Size: 295 279 442 166
Fragments arranged by size:
442 295 279 166
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 986 1182
Size: 986 196
BsrBI CCG'CTC
Cuts at: 0 54 85 152 380 1182
Size: 54 31 67 228 802
Fragments arranged by size:
802 228 67 54 31
BsrFI r'CCGG_y
Cuts at: 0 35 46 422 499 645 1136 1182
Size: 35 11 376 77 146 491 46
Fragments arranged by size:
491 376 146 77 46 35 11
BssHII G'CGCG_C
Cuts at: 0 437 1182
Size: 437 745
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1149 1182
Size: 1149 33
BstEII G'GTnAC_C
Cuts at: 0 648 1182
Size: 648 534
BstYI r'GATC_y
Cuts at: 0 678 1182
Size: 678 504
BtgI C'CryG_G
Cuts at: 0 155 277 1182
Size: 155 122 905
Fragments arranged by size:
905 155 122
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 185 1182
Size: 185 997
EaeI y'GGCC_r
Cuts at: 0 1022 1182
Size: 1022 160
EarI CTCTTCn'nnn_
Cuts at: 0 613 761 788 839 1096 1182
Size: 613 148 27 51 257 86
Fragments arranged by size:
613 257 148 86 51 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 456 984 1182
Size: 456 528 198
Fragments arranged by size:
528 456 198
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 93 424 731 758 823 976 1182
Size: 40 53 331 307 27 65 153 206
Fragments arranged by size:
331 307 206 153 65 53 40 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1060 1182
Size: 1060 122
EcoO109I rG'GnC_Cy
Cuts at: 0 232 246 784 1038 1182
Size: 232 14 538 254 144
Fragments arranged by size:
538 254 232 144 14
HaeII r_GCGC'y
Cuts at: 0 163 514 1000 1182
Size: 163 351 486 182
Fragments arranged by size:
486 351 182 163
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 85 117 840 872 927 959 1182
Size: 85 32 723 32 55 32 223
Fragments arranged by size:
723 223 85 55 32 32 32
KasI G'GCGC_C
Cuts at: 0 159 510 1182
Size: 159 351 672
Fragments arranged by size:
672 351 159
MscI TGG'CCA
Cuts at: 0 1024 1182
Size: 1024 158
MslI CAynn'nnrTG
Cuts at: 0 658 728 1182
Size: 658 70 454
Fragments arranged by size:
658 454 70
MspA1I CmG'CkG
Cuts at: 0 531 1028 1182
Size: 531 497 154
Fragments arranged by size:
531 497 154
NaeI GCC'GGC
Cuts at: 0 37 501 1138 1182
Size: 37 464 637 44
Fragments arranged by size:
637 464 44 37
NarI GG'CG_CC
Cuts at: 0 160 511 1182
Size: 160 351 671
Fragments arranged by size:
671 351 160
NgoMIV G'CCGG_C
Cuts at: 0 35 499 1136 1182
Size: 35 464 637 46
Fragments arranged by size:
637 464 46 35
NruI TCG'CGA
Cuts at: 0 987 1182
Size: 987 195
NspI r_CATG'y
Cuts at: 0 375 1182
Size: 375 807
PasI CC'CwG_GG
Cuts at: 0 221 1182
Size: 221 961
PfoI T'CCnGG_A
Cuts at: 0 953 1182
Size: 953 229
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 334 366 1182
Size: 334 32 816
Fragments arranged by size:
816 334 32
PpuMI rG'GwC_Cy
Cuts at: 0 232 246 784 1182
Size: 232 14 538 398
Fragments arranged by size:
538 398 232 14
PstI C_TGCA'G
Cuts at: 0 819 1182
Size: 819 363
PvuII CAG'CTG
Cuts at: 0 1028 1182
Size: 1028 154
RsrII CG'GwC_CG
Cuts at: 0 49 1182
Size: 49 1133
SanDI GG'GwC_CC
Cuts at: 0 232 246 784 1182
Size: 232 14 538 398
Fragments arranged by size:
538 398 232 14
SexAI A'CCwGG_T
Cuts at: 0 1007 1182
Size: 1007 175
SfcI C'TryA_G
Cuts at: 0 815 866 1112 1182
Size: 815 51 246 70
Fragments arranged by size:
815 246 70 51
SfoI GGC'GCC
Cuts at: 0 161 512 1182
Size: 161 351 670
Fragments arranged by size:
670 351 161
SmaI CCC'GGG
Cuts at: 0 258 316 394 445 1182
Size: 258 58 78 51 737
Fragments arranged by size:
737 258 78 58 51
StuI AGG'CCT
Cuts at: 0 553 1066 1182
Size: 553 513 116
Fragments arranged by size:
553 513 116
StyI C'CwwG_G
Cuts at: 0 458 1034 1067 1182
Size: 458 576 33 115
Fragments arranged by size:
576 458 115 33
XmaI C'CCGG_G
Cuts at: 0 256 314 392 443 1182
Size: 256 58 78 51 739
Fragments arranged by size:
739 256 78 58 51
XmnI GAAnn'nnTTC
Cuts at: 0 184 694 797 1182
Size: 184 510 103 385
Fragments arranged by size:
510 385 184 103
Enzymes that do cut:
AcuI AlfI AloI AlwNI ApaLI ApoI AscI AvaI
BamHI BanI BanII BbeI BbvCI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI
BssHII BstAPI BstEII BstYI BtgI BtgZI EaeI EarI
EciI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MscI
MslI MspA1I NaeI NarI NgoMIV NruI NspI PasI
PfoI PpiI PpuMI PstI PvuII RsrII SanDI SexAI
SfcI SfoI SmaI StuI StyI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI ApaI AseI AsiSI AvrII BaeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
BmtI BpuEI BsaI BsaAI BsaBI BsiWI BsmI BspEI
BspHI BsrDI BsrGI BssSI BstBI BstXI BstZ17I Bsu36I
BtsI ClaI CspCI DraI DraIII DrdI EagI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MmeI NcoI NdeI NheI
NotI NsiI PacI PciI PflMI PmeI PmlI PshAI
PsiI PspOMI PspXI PsrI PvuI SacI SacII SalI
SapI SbfI ScaI SfiI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XhoI ZraI