Restriction map for
pF1KB7944
(Linear) (Six-base) MAPSORT of: KIBB7944.seq Check: 9567 from: 1 to: 738
KIBB7944.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AatII G_ACGT'C
Cuts at: 0 239 738
Size: 239 499
AlwNI CAG_nnn'CTG
Cuts at: 0 216 250 738
Size: 216 34 488
Fragments arranged by size:
488 216 34
ApaI G_GGCC'C
Cuts at: 0 73 738
Size: 73 665
AvaI C'yCGr_G
Cuts at: 0 66 738
Size: 66 672
BamHI G'GATC_C
Cuts at: 0 118 738
Size: 118 620
BanI G'GyrC_C
Cuts at: 0 334 355 738
Size: 334 21 383
Fragments arranged by size:
383 334 21
BanII G_rGCy'C
Cuts at: 0 54 73 157 725 738
Size: 54 19 84 568 13
Fragments arranged by size:
568 84 54 19 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 488 738
Size: 488 250
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 213 247 738
Size: 213 34 491
Fragments arranged by size:
491 213 34
BglI GCCn_nnn'nGGC
Cuts at: 0 29 738
Size: 29 709
BglII A'GATC_T
Cuts at: 0 701 738
Size: 701 37
Bme1580I G_kGCm'C
Cuts at: 0 73 360 738
Size: 73 287 378
Fragments arranged by size:
378 287 73
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 171 738
Size: 171 567
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 181 454 718 738
Size: 124 57 273 264 20
Fragments arranged by size:
273 264 124 57 20
BsaHI Gr'CG_yC
Cuts at: 0 236 738
Size: 236 502
BsaWI w'CCGG_w
Cuts at: 0 380 738
Size: 380 358
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 574 604 738
Size: 574 30 134
Fragments arranged by size:
574 134 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 163 178 723 738
Size: 163 15 545 15
Fragments arranged by size:
545 163 15 15
BseYI C'CCAG_C
Cuts at: 0 55 72 327 738
Size: 55 17 255 411
Fragments arranged by size:
411 255 55 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 270 738
Size: 270 468
Bsp1286I G_dGCh'C
Cuts at: 0 54 73 157 360 725 738
Size: 54 19 84 203 365 13
Fragments arranged by size:
365 203 84 54 19 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 647 738
Size: 647 91
BsrBI CCG'CTC
Cuts at: 0 14 738
Size: 14 724
BsrDI GCAATG_nn'
Cuts at: 0 198 738
Size: 198 540
BssSI C'ACGA_G
Cuts at: 0 588 738
Size: 588 150
BstAPI GCAn_nnn'nTGC
Cuts at: 0 713 738
Size: 713 25
BstXI CCAn_nnnn'nTGG
Cuts at: 0 193 738
Size: 193 545
BstYI r'GATC_y
Cuts at: 0 118 461 701 738
Size: 118 343 240 37
Fragments arranged by size:
343 240 118 37
Bsu36I CC'TnA_GG
Cuts at: 0 266 738
Size: 266 472
BtgI C'CryG_G
Cuts at: 0 686 738
Size: 686 52
BtsI GCAGTG_nn'
Cuts at: 0 495 738
Size: 495 243
DrdI GACnn_nn'nnGTC
Cuts at: 0 235 490 738
Size: 235 255 248
Fragments arranged by size:
255 248 235
EaeI y'GGCC_r
Cuts at: 0 15 312 738
Size: 15 297 426
Fragments arranged by size:
426 297 15
EarI CTCTTCn'nnn_
Cuts at: 0 421 738
Size: 421 317
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 181 454 718 738
Size: 124 57 273 264 20
Fragments arranged by size:
273 264 124 57 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 163 738
Size: 163 575
EcoO109I rG'GnC_Cy
Cuts at: 0 213 738
Size: 213 525
HaeII r_GCGC'y
Cuts at: 0 24 738
Size: 24 714
MscI TGG'CCA
Cuts at: 0 314 738
Size: 314 424
MspA1I CmG'CkG
Cuts at: 0 688 738
Size: 688 50
NspI r_CATG'y
Cuts at: 0 260 738
Size: 260 478
PasI CC'CwG_GG
Cuts at: 0 210 360 738
Size: 210 150 378
Fragments arranged by size:
378 210 150
PflMI CCAn_nnn'nTGG
Cuts at: 0 216 738
Size: 216 522
PfoI T'CCnGG_A
Cuts at: 0 548 665 738
Size: 548 117 73
Fragments arranged by size:
548 117 73
PspOMI G'GGCC_C
Cuts at: 0 69 738
Size: 69 669
PstI C_TGCA'G
Cuts at: 0 577 738
Size: 577 161
SacII CC_GC'GG
Cuts at: 0 689 738
Size: 689 49
SexAI A'CCwGG_T
Cuts at: 0 5 738
Size: 5 733
SfcI C'TryA_G
Cuts at: 0 573 738
Size: 573 165
SmaI CCC'GGG
Cuts at: 0 68 738
Size: 68 670
StyI C'CwwG_G
Cuts at: 0 186 278 371 738
Size: 186 92 93 367
Fragments arranged by size:
367 186 93 92
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 352 413 738
Size: 352 61 325
Fragments arranged by size:
352 325 61
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 574 606 738
Size: 574 32 132
Fragments arranged by size:
574 132 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 25 738
Size: 25 713
XmaI C'CCGG_G
Cuts at: 0 66 738
Size: 66 672
ZraI GAC'GTC
Cuts at: 0 237 738
Size: 237 501
Enzymes that do cut:
AatII AlwNI ApaI AvaI BamHI BanI BanII BbsI
BcgI BglI BglII Bme1580I BplI BpmI BsaHI BsaWI
BsaXI BseRI BseYI BsgI Bsp1286I BspMI BsrBI BsrDI
BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI DrdI
EaeI EarI Eco57MI EcoNI EcoO109I HaeII MscI MspA1I
NspI PasI PflMI PfoI PspOMI PstI SacII SexAI
SfcI SmaI StyI TaqII TstI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BbeI BbvCI BciVI BclI
BfrBI BlpI BmgBI BmrI BmtI Bpu10I BpuEI BsaI
BsaAI BsaBI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI
BspHI BsrFI BsrGI BssHII BstBI BstEII BstZ17I BtgZI
ClaI CspCI DraI DraIII EagI EciI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
Tth111I XbaI XhoI XmnI