Restriction map for
pF1KB7940
(Linear) (Six-base) MAPSORT of: KIBB7940.seq Check: 4873 from: 1 to: 720
KIBB7940.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 352 720
Size: 46 306 368
Fragments arranged by size:
368 306 46
ApaLI G'TGCA_C
Cuts at: 0 511 720
Size: 511 209
AvaI C'yCGr_G
Cuts at: 0 419 720
Size: 419 301
BbsI GAAGACnn'nnnn_
Cuts at: 0 166 455 720
Size: 166 289 265
Fragments arranged by size:
289 265 166
BglII A'GATC_T
Cuts at: 0 327 720
Size: 327 393
Bme1580I G_kGCm'C
Cuts at: 0 515 720
Size: 515 205
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 165 720
Size: 165 555
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 720
Size: 155 565
BsaI GGTCTCn'nnnn_
Cuts at: 0 585 720
Size: 585 135
BsaAI yAC'GTr
Cuts at: 0 560 720
Size: 560 160
BsaBI GATnn'nnATC
Cuts at: 0 127 720
Size: 127 593
BsaWI w'CCGG_w
Cuts at: 0 367 720
Size: 367 353
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 153 183 565 595 720
Size: 153 30 382 30 125
Fragments arranged by size:
382 153 125 30 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 720
Size: 268 452
BsiEI CG_ry'CG
Cuts at: 0 8 720
Size: 8 712
BsiHKAI G_wGCw'C
Cuts at: 0 26 515 720
Size: 26 489 205
Fragments arranged by size:
489 205 26
Bsp1286I G_dGCh'C
Cuts at: 0 26 515 720
Size: 26 489 205
Fragments arranged by size:
489 205 26
BspEI T'CCGG_A
Cuts at: 0 367 720
Size: 367 353
BssHII G'CGCG_C
Cuts at: 0 313 720
Size: 313 407
BstBI TT'CG_AA
Cuts at: 0 291 720
Size: 291 429
BstXI CCAn_nnnn'nTGG
Cuts at: 0 18 629 720
Size: 18 611 91
Fragments arranged by size:
611 91 18
BstYI r'GATC_y
Cuts at: 0 200 327 720
Size: 200 127 393
Fragments arranged by size:
393 200 127
BtgI C'CryG_G
Cuts at: 0 152 539 688 720
Size: 152 387 149 32
Fragments arranged by size:
387 152 149 32
BtsI GCAGTG_nn'
Cuts at: 0 357 720
Size: 357 363
DraIII CAC_nnn'GTG
Cuts at: 0 694 720
Size: 694 26
EaeI y'GGCC_r
Cuts at: 0 254 720
Size: 254 466
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 155 352 720
Size: 46 109 197 368
Fragments arranged by size:
368 197 109 46
EcoO109I rG'GnC_Cy
Cuts at: 0 416 720
Size: 416 304
HaeII r_GCGC'y
Cuts at: 0 192 720
Size: 192 528
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 153 185 720
Size: 153 32 535
Fragments arranged by size:
535 153 32
MfeI C'AATT_G
Cuts at: 0 623 720
Size: 623 97
MscI TGG'CCA
Cuts at: 0 256 720
Size: 256 464
MslI CAynn'nnrTG
Cuts at: 0 538 720
Size: 538 182
MspA1I CmG'CkG
Cuts at: 0 78 252 720
Size: 78 174 468
Fragments arranged by size:
468 174 78
NspI r_CATG'y
Cuts at: 0 537 720
Size: 537 183
PflMI CCAn_nnn'nTGG
Cuts at: 0 183 720
Size: 183 537
PfoI T'CCnGG_A
Cuts at: 0 698 720
Size: 698 22
PpuMI rG'GwC_Cy
Cuts at: 0 416 720
Size: 416 304
PstI C_TGCA'G
Cuts at: 0 141 197 720
Size: 141 56 523
Fragments arranged by size:
523 141 56
PvuII CAG'CTG
Cuts at: 0 78 252 720
Size: 78 174 468
Fragments arranged by size:
468 174 78
SanDI GG'GwC_CC
Cuts at: 0 416 720
Size: 416 304
SexAI A'CCwGG_T
Cuts at: 0 575 720
Size: 575 145
SfcI C'TryA_G
Cuts at: 0 137 193 213 720
Size: 137 56 20 507
Fragments arranged by size:
507 137 56 20
SmaI CCC'GGG
Cuts at: 0 421 720
Size: 421 299
TatI w'GTAC_w
Cuts at: 0 14 143 720
Size: 14 129 577
Fragments arranged by size:
577 129 14
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 565 597 680 712 720
Size: 565 32 83 32 8
Fragments arranged by size:
565 83 32 32 8
Tth111I GACn'n_nGTC
Cuts at: 0 165 720
Size: 165 555
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 583 720
Size: 583 137
XmaI C'CCGG_G
Cuts at: 0 419 720
Size: 419 301
Enzymes that do cut:
AcuI ApaLI AvaI BbsI BglII Bme1580I BplI BpmI
BsaI BsaAI BsaBI BsaWI BsaXI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BssHII BstBI BstXI BstYI BtgI BtsI
DraIII EaeI Eco57MI EcoO109I HaeII Hin4I MfeI MscI
MslI MspA1I NspI PflMI PfoI PpuMI PstI PvuII
SanDI SexAI SfcI SmaI TatI TstI Tth111I XcmI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI BfrBI BglI BlpI
BmgBI BmrI BmtI Bpu10I BpuEI BsaHI BseRI BseYI
BsiWI BsmI BsmBI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstEII BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DrdI EagI EarI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MluI MmeI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TaqII XbaI
XhoI XmnI ZraI