Restriction map for
pF1KB7939
(Linear) (Six-base) MAPSORT of: KIBB7939.seq Check: 3621 from: 1 to: 1026
KIBB7939.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 795 1026
Size: 795 231
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 133 269 1026
Size: 68 65 136 757
Fragments arranged by size:
757 136 68 65
AlwNI CAG_nnn'CTG
Cuts at: 0 91 734 1026
Size: 91 643 292
Fragments arranged by size:
643 292 91
ApoI r'AATT_y
Cuts at: 0 142 662 1026
Size: 142 520 364
Fragments arranged by size:
520 364 142
AvaI C'yCGr_G
Cuts at: 0 272 1026
Size: 272 754
BamHI G'GATC_C
Cuts at: 0 123 712 1026
Size: 123 589 314
Fragments arranged by size:
589 314 123
BanI G'GyrC_C
Cuts at: 0 686 1026
Size: 686 340
BanII G_rGCy'C
Cuts at: 0 532 1026
Size: 532 494
BbsI GAAGACnn'nnnn_
Cuts at: 0 10 74 257 500 1026
Size: 10 64 183 243 526
Fragments arranged by size:
526 243 183 64 10
BglII A'GATC_T
Cuts at: 0 287 748 1026
Size: 287 461 278
Fragments arranged by size:
461 287 278
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 999 1026
Size: 999 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 626 1026
Size: 626 400
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 729 790 981 1026
Size: 729 61 191 45
Fragments arranged by size:
729 191 61 45
BsmI GAATG_Cn'
Cuts at: 0 31 1026
Size: 31 995
Bsp1286I G_dGCh'C
Cuts at: 0 532 1026
Size: 532 494
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 795 1026
Size: 795 231
BssSI C'ACGA_G
Cuts at: 0 956 1026
Size: 956 70
BstEII G'GTnAC_C
Cuts at: 0 63 1026
Size: 63 963
BstYI r'GATC_y
Cuts at: 0 123 287 712 748 1026
Size: 123 164 425 36 278
Fragments arranged by size:
425 278 164 123 36
Bsu36I CC'TnA_GG
Cuts at: 0 857 1026
Size: 857 169
BtgI C'CryG_G
Cuts at: 0 234 717 1026
Size: 234 483 309
Fragments arranged by size:
483 309 234
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 415 1026
Size: 415 611
EaeI y'GGCC_r
Cuts at: 0 919 1026
Size: 919 107
EarI CTCTTCn'nnn_
Cuts at: 0 884 1026
Size: 884 142
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 133 269 999 1026
Size: 68 65 136 730 27
Fragments arranged by size:
730 136 68 65 27
EcoO109I rG'GnC_Cy
Cuts at: 0 88 1026
Size: 88 938
EcoRI G'AATT_C
Cuts at: 0 662 1026
Size: 662 364
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 309 618 1026
Size: 309 309 408
Fragments arranged by size:
408 309 309
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 305 337 397 429 724 756 1026
Size: 305 32 60 32 295 32 270
Fragments arranged by size:
305 295 270 60 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 474 1026
Size: 245 229 552
Fragments arranged by size:
552 245 229
MscI TGG'CCA
Cuts at: 0 921 1026
Size: 921 105
MslI CAynn'nnrTG
Cuts at: 0 458 1026
Size: 458 568
MspA1I CmG'CkG
Cuts at: 0 1010 1026
Size: 1010 16
NcoI C'CATG_G
Cuts at: 0 234 1026
Size: 234 792
NspI r_CATG'y
Cuts at: 0 463 710 1026
Size: 463 247 316
Fragments arranged by size:
463 316 247
PfoI T'CCnGG_A
Cuts at: 0 976 1026
Size: 976 50
PstI C_TGCA'G
Cuts at: 0 795 841 874 1026
Size: 795 46 33 152
Fragments arranged by size:
795 152 46 33
PvuII CAG'CTG
Cuts at: 0 1010 1026
Size: 1010 16
SfcI C'TryA_G
Cuts at: 0 791 837 870 1026
Size: 791 46 33 156
Fragments arranged by size:
791 156 46 33
SmaI CCC'GGG
Cuts at: 0 274 1026
Size: 274 752
SmlI C'TyrA_G
Cuts at: 0 641 1026
Size: 641 385
SrfI GCCC'GGGC
Cuts at: 0 274 1026
Size: 274 752
StyI C'CwwG_G
Cuts at: 0 234 884 986 1026
Size: 234 650 102 40
Fragments arranged by size:
650 234 102 40
TatI w'GTAC_w
Cuts at: 0 407 1026
Size: 407 619
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 431 463 1026
Size: 431 32 563
Fragments arranged by size:
563 431 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 364 680 1026
Size: 364 316 346
Fragments arranged by size:
364 346 316
XmaI C'CCGG_G
Cuts at: 0 272 1026
Size: 272 754
XmnI GAAnn'nnTTC
Cuts at: 0 146 1026
Size: 146 880
Enzymes that do cut:
AarI AcuI AlwNI ApoI AvaI BamHI BanI BanII
BbsI BglII BpmI BpuEI BseRI BsmI Bsp1286I BspMI
BssSI BstEII BstYI Bsu36I BtgI BtgZI EaeI EarI
Eco57MI EcoO109I EcoRI FalI Hin4I MmeI MscI MslI
MspA1I NcoI NspI PfoI PstI PvuII SfcI SmaI
SmlI SrfI StyI TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI
Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstXI BstZ17I
BtsI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRV FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SspI StuI SwaI TaqII
Tth111I XbaI XhoI ZraI