Restriction map for
pF1KB7938
(Linear) (Six-base) MAPSORT of: KIBB7938.seq Check: 6883 from: 1 to: 699
KIBB7938.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 0 77 699
Size: 77 622
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 176 699
Size: 62 114 523
Fragments arranged by size:
523 114 62
AflII C'TTAA_G
Cuts at: 0 672 699
Size: 672 27
AflIII A'CryG_T
Cuts at: 0 72 699
Size: 72 627
AleI CACnn'nnGTG
Cuts at: 0 43 699
Size: 43 656
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 382 414 699
Size: 382 32 285
Fragments arranged by size:
382 285 32
ApoI r'AATT_y
Cuts at: 0 66 161 408 516 699
Size: 66 95 247 108 183
Fragments arranged by size:
247 183 108 95 66
BanII G_rGCy'C
Cuts at: 0 8 600 699
Size: 8 592 99
Fragments arranged by size:
592 99 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 155 699
Size: 155 544
BclI T'GATC_A
Cuts at: 0 114 699
Size: 114 585
BglII A'GATC_T
Cuts at: 0 615 699
Size: 615 84
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 384 699
Size: 384 315
BsaAI yAC'GTr
Cuts at: 0 73 124 682 699
Size: 73 51 558 17
Fragments arranged by size:
558 73 51 17
BsaHI Gr'CG_yC
Cuts at: 0 56 487 699
Size: 56 431 212
Fragments arranged by size:
431 212 56
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 382 412 699
Size: 382 30 287
Fragments arranged by size:
382 287 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 43 590 699
Size: 43 547 109
Fragments arranged by size:
547 109 43
Bsp1286I G_dGCh'C
Cuts at: 0 8 600 699
Size: 8 592 99
Fragments arranged by size:
592 99 8
BstYI r'GATC_y
Cuts at: 0 615 699
Size: 615 84
BstZ17I GTA'TAC
Cuts at: 0 78 699
Size: 78 621
EarI CTCTTCn'nnn_
Cuts at: 0 567 699
Size: 567 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 176 384 699
Size: 62 114 208 315
Fragments arranged by size:
315 208 114 62
EcoNI CCTnn'n_nnAGG
Cuts at: 0 25 699
Size: 25 674
EcoO109I rG'GnC_Cy
Cuts at: 0 33 588 699
Size: 33 555 111
Fragments arranged by size:
555 111 33
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 75 699
Size: 75 624
MslI CAynn'nnrTG
Cuts at: 0 43 122 454 699
Size: 43 79 332 245
Fragments arranged by size:
332 245 79 43
PasI CC'CwG_GG
Cuts at: 0 592 699
Size: 592 107
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 382 414 699
Size: 382 32 285
Fragments arranged by size:
382 285 32
PpuMI rG'GwC_Cy
Cuts at: 0 33 588 699
Size: 33 555 111
Fragments arranged by size:
555 111 33
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 534 566 699
Size: 534 32 133
Fragments arranged by size:
534 133 32
SanDI GG'GwC_CC
Cuts at: 0 588 699
Size: 588 111
SfcI C'TryA_G
Cuts at: 0 619 699
Size: 619 80
SmlI C'TyrA_G
Cuts at: 0 672 699
Size: 672 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 40 699
Size: 40 659
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 263 295 699
Size: 263 32 404
Fragments arranged by size:
404 263 32
XbaI T'CTAG_A
Cuts at: 0 528 699
Size: 528 171
XmnI GAAnn'nnTTC
Cuts at: 0 254 371 699
Size: 254 117 328
Fragments arranged by size:
328 254 117
Enzymes that do cut:
AccI AcuI AflII AflIII AleI AloI ApoI BanII
BbsI BclI BglII BpmI BsaAI BsaHI BsaXI BseRI
Bsp1286I BstYI BstZ17I EarI Eco57MI EcoNI EcoO109I FalI
MslI PasI PpiI PpuMI PsrI SanDI SfcI SmlI
TaqII TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AlfI
AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BciVI BfrBI
BglI BlpI Bme1580I BmgBI BmrI BmtI BplI Bpu10I
BpuEI BsaI BsaBI BsaWI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EciI EcoICRI EcoRI EcoRV FseI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PflMI PfoI PmeI PmlI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI TatI Tth111I XcmI XhoI XmaI ZraI