Restriction map for
pF1KB7935
(Linear) (Six-base) MAPSORT of: KIBB7935.seq Check: 1161 from: 1 to: 1113
KIBB7935.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AatII G_ACGT'C
Cuts at: 0 587 1113
Size: 587 526
AccI GT'mk_AC
Cuts at: 0 1052 1113
Size: 1052 61
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 1113
Size: 27 1086
AlwNI CAG_nnn'CTG
Cuts at: 0 216 504 601 1113
Size: 216 288 97 512
Fragments arranged by size:
512 288 216 97
ApaI G_GGCC'C
Cuts at: 0 473 1113
Size: 473 640
AvaI C'yCGr_G
Cuts at: 0 781 1113
Size: 781 332
BamHI G'GATC_C
Cuts at: 0 657 714 1113
Size: 657 57 399
Fragments arranged by size:
657 399 57
BanI G'GyrC_C
Cuts at: 0 295 814 1113
Size: 295 519 299
Fragments arranged by size:
519 299 295
BanII G_rGCy'C
Cuts at: 0 407 473 564 619 863 1113
Size: 407 66 91 55 244 250
Fragments arranged by size:
407 250 244 91 66 55
BbeI G_GCGC'C
Cuts at: 0 299 1113
Size: 299 814
BbsI GAAGACnn'nnnn_
Cuts at: 0 211 897 1113
Size: 211 686 216
Fragments arranged by size:
686 216 211
BlpI GC'TnA_GC
Cuts at: 0 757 985 1113
Size: 757 228 128
Fragments arranged by size:
757 228 128
Bme1580I G_kGCm'C
Cuts at: 0 318 473 1113
Size: 318 155 640
Fragments arranged by size:
640 318 155
BmgBI CAC'GTC
Cuts at: 0 648 1113
Size: 648 465
BmrI ACTGGGnnnn_n'
Cuts at: 0 186 780 1113
Size: 186 594 333
Fragments arranged by size:
594 333 186
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 1084 1113
Size: 448 636 29
Fragments arranged by size:
636 448 29
Bpu10I CC'TnA_GC
Cuts at: 0 130 1113
Size: 130 983
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 1056 1113
Size: 340 716 57
Fragments arranged by size:
716 340 57
BsaI GGTCTCn'nnnn_
Cuts at: 0 371 1113
Size: 371 742
BsaHI Gr'CG_yC
Cuts at: 0 296 584 902 1113
Size: 296 288 318 211
Fragments arranged by size:
318 296 288 211
BsaWI w'CCGG_w
Cuts at: 0 634 1113
Size: 634 479
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 337 615 1113
Size: 337 278 498
Fragments arranged by size:
498 337 278
BseYI C'CCAG_C
Cuts at: 0 368 682 1113
Size: 368 314 431
Fragments arranged by size:
431 368 314
BsiHKAI G_wGCw'C
Cuts at: 0 407 619 863 1113
Size: 407 212 244 250
Fragments arranged by size:
407 250 244 212
BsmBI CGTCTCn'nnnn_
Cuts at: 0 591 1113
Size: 591 522
Bsp1286I G_dGCh'C
Cuts at: 0 318 407 473 564 619 863 1113
Size: 318 89 66 91 55 244 250
Fragments arranged by size:
318 250 244 91 89 66 55
BspHI T'CATG_A
Cuts at: 0 875 1113
Size: 875 238
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 201 1113
Size: 201 912
BsrFI r'CCGG_y
Cuts at: 0 743 1040 1113
Size: 743 297 73
Fragments arranged by size:
743 297 73
BsrGI T'GTAC_A
Cuts at: 0 53 1113
Size: 53 1060
BstXI CCAn_nnnn'nTGG
Cuts at: 0 177 1113
Size: 177 936
BstYI r'GATC_y
Cuts at: 0 657 714 1019 1113
Size: 657 57 305 94
Fragments arranged by size:
657 305 94 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 64 701 1113
Size: 64 637 412
Fragments arranged by size:
637 412 64
DraIII CAC_nnn'GTG
Cuts at: 0 283 1113
Size: 283 830
EaeI y'GGCC_r
Cuts at: 0 1038 1113
Size: 1038 75
EarI CTCTTCn'nnn_
Cuts at: 0 399 682 787 1085 1113
Size: 399 283 105 298 28
Fragments arranged by size:
399 298 283 105 28
EcoICRI GAG'CTC
Cuts at: 0 405 617 861 1113
Size: 405 212 244 252
Fragments arranged by size:
405 252 244 212
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 448 1084 1113
Size: 27 421 636 29
Fragments arranged by size:
636 421 29 27
EcoO109I rG'GnC_Cy
Cuts at: 0 456 469 470 870 1113
Size: 456 13 1 400 243
Fragments arranged by size:
456 400 243 13 1
FseI GG_CCGG'CC
Cuts at: 0 747 1113
Size: 747 366
HaeII r_GCGC'y
Cuts at: 0 266 299 1113
Size: 266 33 814
Fragments arranged by size:
814 266 33
KasI G'GCGC_C
Cuts at: 0 295 1113
Size: 295 818
MfeI C'AATT_G
Cuts at: 0 309 1113
Size: 309 804
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 1087 1113
Size: 391 696 26
Fragments arranged by size:
696 391 26
MspA1I CmG'CkG
Cuts at: 0 173 372 548 1113
Size: 173 199 176 565
Fragments arranged by size:
565 199 176 173
NaeI GCC'GGC
Cuts at: 0 745 1042 1113
Size: 745 297 71
Fragments arranged by size:
745 297 71
NarI GG'CG_CC
Cuts at: 0 296 1113
Size: 296 817
NgoMIV G'CCGG_C
Cuts at: 0 743 1040 1113
Size: 743 297 73
Fragments arranged by size:
743 297 73
PasI CC'CwG_GG
Cuts at: 0 466 1113
Size: 466 647
PfoI T'CCnGG_A
Cuts at: 0 449 836 1022 1113
Size: 449 387 186 91
Fragments arranged by size:
449 387 186 91
PshAI GACnn'nnGTC
Cuts at: 0 515 1113
Size: 515 598
PspOMI G'GGCC_C
Cuts at: 0 469 1113
Size: 469 644
PvuII CAG'CTG
Cuts at: 0 173 372 548 1113
Size: 173 199 176 565
Fragments arranged by size:
565 199 176 173
SacI G_AGCT'C
Cuts at: 0 407 619 863 1113
Size: 407 212 244 250
Fragments arranged by size:
407 250 244 212
SapI GCTCTTCn'nnn_
Cuts at: 0 399 682 787 1113
Size: 399 283 105 326
Fragments arranged by size:
399 326 283 105
ScaI AGT'ACT
Cuts at: 0 769 1113
Size: 769 344
SfoI GGC'GCC
Cuts at: 0 297 1113
Size: 297 816
SmlI C'TyrA_G
Cuts at: 0 319 781 1071 1113
Size: 319 462 290 42
Fragments arranged by size:
462 319 290 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 832 1113
Size: 832 281
TatI w'GTAC_w
Cuts at: 0 53 767 1113
Size: 53 714 346
Fragments arranged by size:
714 346 53
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 632 664 1113
Size: 632 32 449
Fragments arranged by size:
632 449 32
XhoI C'TCGA_G
Cuts at: 0 781 1113
Size: 781 332
XmnI GAAnn'nnTTC
Cuts at: 0 1094 1113
Size: 1094 19
ZraI GAC'GTC
Cuts at: 0 585 1113
Size: 585 528
Enzymes that do cut:
AatII AccI AcuI AlwNI ApaI AvaI BamHI BanI
BanII BbeI BbsI BlpI Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BseRI BseYI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrFI BsrGI BstXI BstYI
BtgZI DraIII EaeI EarI EcoICRI Eco57MI EcoO109I FseI
HaeII KasI MfeI MmeI MspA1I NaeI NarI NgoMIV
PasI PfoI PshAI PspOMI PvuII SacI SapI ScaI
SfoI SmlI TaqII TatI TstI XhoI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BmtI BplI BsaAI BsaBI BsaXI BsgI BsiEI
BsiWI BsmI BspEI BsrBI BsrDI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgI BtsI ClaI CspCI
DraI DrdI EagI EciI EcoNI EcoRI EcoRV FalI
FspI FspAI Hin4I HincII HindIII HpaI KpnI MluI
MscI MslI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PciI PflMI PmeI PmlI PpiI PpuMI
PsiI PspXI PsrI PstI PvuI RsrII SacII SalI
SanDI SbfI SexAI SfcI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
Tth111I XbaI XcmI XmaI