(Linear) (Six-base) MAPSORT of: KIBB7934.seq Check: 1653 from: 1 to: 645 KIBB7934.orf With 182 enzymes: SgfI * October 7, 2009 14:25 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 86 472 645 Size: 86 386 173 Fragments arranged by size: 386 173 86 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 113 645 Size: 113 532 ApoI r'AATT_y Cuts at: 0 175 645 Size: 175 470 BamHI G'GATC_C Cuts at: 0 270 645 Size: 270 375 BbsI GAAGACnn'nnnn_ Cuts at: 0 154 645 Size: 154 491 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 49 83 645 Size: 49 34 562 Fragments arranged by size: 562 49 34 BfrBI ATG'CAT Cuts at: 0 49 645 Size: 49 596 BmrI ACTGGGnnnn_n' Cuts at: 0 394 645 Size: 394 251 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 88 574 604 607 610 645 Size: 88 486 30 3 3 35 Fragments arranged by size: 486 88 35 30 3 3 BsiHKAI G_wGCw'C Cuts at: 0 136 645 Size: 136 509 Bsp1286I G_dGCh'C Cuts at: 0 136 645 Size: 136 509 BstYI r'GATC_y Cuts at: 0 12 270 645 Size: 12 258 375 Fragments arranged by size: 375 258 12 BtsI GCAGTG_nn' Cuts at: 0 404 645 Size: 404 241 EarI CTCTTCn'nnn_ Cuts at: 0 316 551 575 645 Size: 316 235 24 70 Fragments arranged by size: 316 235 70 24 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 86 472 645 Size: 86 386 173 Fragments arranged by size: 386 173 86 EcoNI CCTnn'n_nnAGG Cuts at: 0 242 645 Size: 242 403 HincII GTy'rAC Cuts at: 0 108 645 Size: 108 537 MslI CAynn'nnrTG Cuts at: 0 95 645 Size: 95 550 NdeI CA'TA_TG Cuts at: 0 45 645 Size: 45 600 NsiI A_TGCA'T Cuts at: 0 51 645 Size: 51 594 PstI C_TGCA'G Cuts at: 0 414 645 Size: 414 231 SfcI C'TryA_G Cuts at: 0 410 645 Size: 410 235 StuI AGG'CCT Cuts at: 0 291 645 Size: 291 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 107 297 645 Size: 107 190 348 Fragments arranged by size: 348 190 107 Tth111I GACn'n_nGTC Cuts at: 0 153 645 Size: 153 492 Enzymes that do cut: AcuI AlfI ApoI BamHI BbsI BcgI BfrBI BmrI BseRI BsiHKAI Bsp1286I BstYI BtsI EarI Eco57MI EcoNI HincII MslI NdeI NsiI PstI SfcI StuI TaqII Tth111I Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BanI BanII BbeI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MspA1I NaeI NarI NcoI NgoMIV NheI NotI NruI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StyI SwaI TatI TstI XbaI XcmI XhoI XmaI XmnI ZraI