(Linear) (Six-base) MAPSORT of: KIBB7930.seq  Check: 763  from: 1  to: 639



KIBB7930.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:25  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     352     639 
   Size:        352     287
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     414     639 
   Size:        414     225
 
BglII A'GATC_T

Cuts at:      0     273     639 
   Size:        273     366
 
Bme1580I G_kGCm'C

Cuts at:      0     233     639 
   Size:        233     406
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      83     639 
   Size:         83     556
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     161     301     639 
   Size:        161     140     338

  Fragments arranged by size: 

                 338     161     140
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     472     639 
   Size:        472     167
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      25     639 
   Size:         25     614
 
BsiHKAI G_wGCw'C

Cuts at:      0     170     639 
   Size:        170     469
 
BsmI GAATG_Cn'

Cuts at:      0     228     639 
   Size:        228     411
 
Bsp1286I G_dGCh'C

Cuts at:      0     170     233     639 
   Size:        170      63     406

  Fragments arranged by size: 

                 406     170      63
 
BstYI r'GATC_y

Cuts at:      0     273     639 
   Size:        273     366
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     519     639 
   Size:        519     120
 
DraI TTT'AAA

Cuts at:      0     135     639 
   Size:        135     504
 
EarI CTCTTCn'nnn_

Cuts at:      0     371     639 
   Size:        371     268
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0       6     639 
   Size:          6     633
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     161     301     352     639 
   Size:        161     140      51     287

  Fragments arranged by size: 

                 287     161     140      51
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     292     639 
   Size:        292     347
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     562     594     639 
   Size:        562      32      45

  Fragments arranged by size: 

                 562      45      32
 
MspA1I CmG'CkG

Cuts at:      0     115     435     639 
   Size:        115     320     204

  Fragments arranged by size: 

                 320     204     115
 
PvuII CAG'CTG

Cuts at:      0     115     639 
   Size:        115     524
 
SmlI C'TyrA_G

Cuts at:      0     487     639 
   Size:        487     152
 
SspI AAT'ATT

Cuts at:      0     239     639 
   Size:        239     400
 
TatI w'GTAC_w

Cuts at:      0     247     639 
   Size:        247     392
 
Tth111I GACn'n_nGTC

Cuts at:      0      89     639 
   Size:         89     550
 
XmnI GAAnn'nnTTC

Cuts at:      0     275     296     639 
   Size:        275      21     343

  Fragments arranged by size: 

                 343     275      21
 

 Enzymes that do cut:

     AcuI    BciVI    BglII Bme1580I     BmrI     BpmI    BpuEI     BsgI
  BsiHKAI     BsmI Bsp1286I    BstYI    BtgZI     DraI     EarI     EciI
  Eco57MI     FalI    Hin4I   MspA1I    PvuII     SmlI     SspI     TatI
  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI
     BglI     BlpI    BmgBI     BmtI     BplI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI    BsiWI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     StuI     StyI     SwaI    TaqII     TstI     XbaI     XcmI     XhoI
     XmaI     ZraI