Restriction map for
pF1KB7910
(Linear) (Six-base) MAPSORT of: KIBB7910.seq Check: 3151 from: 1 to: 990
KIBB7910.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 722 990
Size: 722 268
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 438 837 894 990
Size: 438 399 57 96
Fragments arranged by size:
438 399 96 57
AfeI AGC'GCT
Cuts at: 0 691 990
Size: 691 299
AleI CACnn'nnGTG
Cuts at: 0 812 990
Size: 812 178
AlwNI CAG_nnn'CTG
Cuts at: 0 162 990
Size: 162 828
ApaI G_GGCC'C
Cuts at: 0 244 990
Size: 244 746
ApaLI G'TGCA_C
Cuts at: 0 924 990
Size: 924 66
BanI G'GyrC_C
Cuts at: 0 99 231 962 990
Size: 99 132 731 28
Fragments arranged by size:
731 132 99 28
BanII G_rGCy'C
Cuts at: 0 244 443 533 865 990
Size: 244 199 90 332 125
Fragments arranged by size:
332 244 199 125 90
BbsI GAAGACnn'nnnn_
Cuts at: 0 504 990
Size: 504 486
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 582 616 990
Size: 582 34 374
Fragments arranged by size:
582 374 34
BglI GCCn_nnn'nGGC
Cuts at: 0 223 413 990
Size: 223 190 577
Fragments arranged by size:
577 223 190
Bme1580I G_kGCm'C
Cuts at: 0 104 244 431 928 990
Size: 104 140 187 497 62
Fragments arranged by size:
497 187 140 104 62
BmgBI CAC'GTC
Cuts at: 0 636 990
Size: 636 354
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 412 640 990
Size: 217 195 228 350
Fragments arranged by size:
350 228 217 195
Bpu10I CC'TnA_GC
Cuts at: 0 136 990
Size: 136 854
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 970 990
Size: 970 20
BsaI GGTCTCn'nnnn_
Cuts at: 0 101 990
Size: 101 889
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 625 655 990
Size: 625 30 335
Fragments arranged by size:
625 335 30
BseYI C'CCAG_C
Cuts at: 0 430 990
Size: 430 560
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 990
Size: 79 911
BsiHKAI G_wGCw'C
Cuts at: 0 398 865 928 990
Size: 398 467 63 62
Fragments arranged by size:
467 398 63 62
Bsp1286I G_dGCh'C
Cuts at: 0 104 244 398 431 443 533 865 928
Size: 104 140 154 33 12 90 332 63
Cuts at: 928 990
Size: 62
Fragments arranged by size:
332 154 140 104 90 63 62 33
12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 722 962 990
Size: 722 240 28
Fragments arranged by size:
722 240 28
BsrBI CCG'CTC
Cuts at: 0 75 990
Size: 75 915
BsrFI r'CCGG_y
Cuts at: 0 356 990
Size: 356 634
DraIII CAC_nnn'GTG
Cuts at: 0 495 612 990
Size: 495 117 378
Fragments arranged by size:
495 378 117
EaeI y'GGCC_r
Cuts at: 0 215 332 345 603 990
Size: 215 117 13 258 387
Fragments arranged by size:
387 258 215 117 13
EarI CTCTTCn'nnn_
Cuts at: 0 16 990
Size: 16 974
EcoICRI GAG'CTC
Cuts at: 0 863 990
Size: 863 127
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 412 438 640 837 894 990
Size: 217 195 26 202 197 57 96
Fragments arranged by size:
217 202 197 195 96 57 26
EcoO109I rG'GnC_Cy
Cuts at: 0 240 241 363 563 990
Size: 240 1 122 200 427
Fragments arranged by size:
427 240 200 122 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 330 990
Size: 330 660
HaeII r_GCGC'y
Cuts at: 0 693 990
Size: 693 297
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 259 291 990
Size: 259 32 699
Fragments arranged by size:
699 259 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 806 890 990
Size: 311 495 84 100
Fragments arranged by size:
495 311 100 84
MscI TGG'CCA
Cuts at: 0 217 334 347 990
Size: 217 117 13 643
Fragments arranged by size:
643 217 117 13
MslI CAynn'nnrTG
Cuts at: 0 812 990
Size: 812 178
MspA1I CmG'CkG
Cuts at: 0 914 990
Size: 914 76
NspI r_CATG'y
Cuts at: 0 544 672 990
Size: 544 128 318
Fragments arranged by size:
544 318 128
PflMI CCAn_nnn'nTGG
Cuts at: 0 162 213 990
Size: 162 51 777
Fragments arranged by size:
777 162 51
PfoI T'CCnGG_A
Cuts at: 0 389 990
Size: 389 601
PpuMI rG'GwC_Cy
Cuts at: 0 563 990
Size: 563 427
PspOMI G'GGCC_C
Cuts at: 0 240 990
Size: 240 750
PvuII CAG'CTG
Cuts at: 0 914 990
Size: 914 76
SacI G_AGCT'C
Cuts at: 0 865 990
Size: 865 125
SanDI GG'GwC_CC
Cuts at: 0 563 990
Size: 563 427
SapI GCTCTTCn'nnn_
Cuts at: 0 16 990
Size: 16 974
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 223 990
Size: 223 767
SmlI C'TyrA_G
Cuts at: 0 985 990
Size: 985 5
StuI AGG'CCT
Cuts at: 0 682 850 990
Size: 682 168 140
Fragments arranged by size:
682 168 140
StyI C'CwwG_G
Cuts at: 0 329 446 990
Size: 329 117 544
Fragments arranged by size:
544 329 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 724 892 990
Size: 724 168 98
Fragments arranged by size:
724 168 98
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 28 625 657 763 795 990
Size: 28 597 32 106 32 195
Fragments arranged by size:
597 195 106 32 32 28
Enzymes that do cut:
AarI AcuI AfeI AleI AlwNI ApaI ApaLI BanI
BanII BbsI BcgI BglI Bme1580I BmgBI BpmI Bpu10I
BpuEI BsaI BsaXI BseYI BsgI BsiHKAI Bsp1286I BspMI
BsrBI BsrFI DraIII EaeI EarI EcoICRI Eco57MI EcoO109I
FalI HaeII Hin4I MmeI MscI MslI MspA1I NspI
PflMI PfoI PpuMI PspOMI PvuII SacI SanDI SapI
SfiI SmlI StuI StyI TaqII TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AlfI AloI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BciVI BclI BfrBI BglII
BlpI BmrI BmtI BplI BsaAI BsaBI BsaHI BsaWI
BseRI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EagI EciI EcoNI EcoRI EcoRV FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PstI PvuI RsrII SacII SalI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI