Restriction map for
pF1KB7895
(Linear) (Six-base) MAPSORT of: KIBB7895.seq Check: 3447 from: 1 to: 1764
KIBB7895.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 0 1313 1764
Size: 1313 451
Acc65I G'GTAC_C
Cuts at: 0 1208 1764
Size: 1208 556
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 911 1460 1465 1764
Size: 322 589 549 5 299
Fragments arranged by size:
589 549 322 299 5
AfeI AGC'GCT
Cuts at: 0 331 1764
Size: 331 1433
AflIII A'CryG_T
Cuts at: 0 1459 1575 1764
Size: 1459 116 189
Fragments arranged by size:
1459 189 116
AlwNI CAG_nnn'CTG
Cuts at: 0 1396 1764
Size: 1396 368
ApoI r'AATT_y
Cuts at: 0 1524 1552 1764
Size: 1524 28 212
Fragments arranged by size:
1524 212 28
AvaI C'yCGr_G
Cuts at: 0 7 1764
Size: 7 1757
BanI G'GyrC_C
Cuts at: 0 1208 1764
Size: 1208 556
BbsI GAAGACnn'nnnn_
Cuts at: 0 899 1764
Size: 899 865
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1362 1396 1764
Size: 1362 34 368
Fragments arranged by size:
1362 368 34
BfrBI ATG'CAT
Cuts at: 0 963 1764
Size: 963 801
BglI GCCn_nnn'nGGC
Cuts at: 0 1042 1764
Size: 1042 722
BglII A'GATC_T
Cuts at: 0 696 1764
Size: 696 1068
BmgBI CAC'GTC
Cuts at: 0 781 1764
Size: 781 983
BmrI ACTGGGnnnn_n'
Cuts at: 0 364 438 1764
Size: 364 74 1326
Fragments arranged by size:
1326 364 74
Bpu10I CC'TnA_GC
Cuts at: 0 443 1764
Size: 443 1321
BsaAI yAC'GTr
Cuts at: 0 981 1764
Size: 981 783
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 484 514 1764
Size: 484 30 1250
Fragments arranged by size:
1250 484 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 25 1764
Size: 25 1739
BseYI C'CCAG_C
Cuts at: 0 212 1764
Size: 212 1552
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1749 1764
Size: 1749 15
BsrBI CCG'CTC
Cuts at: 0 490 1764
Size: 490 1274
BsrGI T'GTAC_A
Cuts at: 0 1065 1570 1764
Size: 1065 505 194
Fragments arranged by size:
1065 505 194
BstAPI GCAn_nnn'nTGC
Cuts at: 0 98 1764
Size: 98 1666
BstYI r'GATC_y
Cuts at: 0 571 696 1741 1764
Size: 571 125 1045 23
Fragments arranged by size:
1045 571 125 23
BtgI C'CryG_G
Cuts at: 0 1186 1467 1764
Size: 1186 281 297
Fragments arranged by size:
1186 297 281
BtsI GCAGTG_nn'
Cuts at: 0 1510 1764
Size: 1510 254
DraI TTT'AAA
Cuts at: 0 27 1764
Size: 27 1737
EarI CTCTTCn'nnn_
Cuts at: 0 14 337 710 1436 1639 1764
Size: 14 323 373 726 203 125
Fragments arranged by size:
726 373 323 203 125 14
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 31 1764
Size: 31 1733
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 911 1460 1465 1764
Size: 322 589 549 5 299
Fragments arranged by size:
589 549 322 299 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 10 1764
Size: 10 1754
EcoRI G'AATT_C
Cuts at: 0 1524 1764
Size: 1524 240
EcoRV GAT'ATC
Cuts at: 0 121 1764
Size: 121 1643
HaeII r_GCGC'y
Cuts at: 0 333 1764
Size: 333 1431
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 484 516 1011 1043 1764
Size: 484 32 495 32 721
Fragments arranged by size:
721 495 484 32 32
HincII GTy'rAC
Cuts at: 0 945 1314 1764
Size: 945 369 450
Fragments arranged by size:
945 450 369
HindIII A'AGCT_T
Cuts at: 0 1226 1764
Size: 1226 538
KpnI G_GTAC'C
Cuts at: 0 1212 1764
Size: 1212 552
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 831 1764
Size: 831 933
MslI CAynn'nnrTG
Cuts at: 0 1087 1764
Size: 1087 677
MspA1I CmG'CkG
Cuts at: 0 464 1764
Size: 464 1300
NcoI C'CATG_G
Cuts at: 0 1186 1467 1764
Size: 1186 281 297
Fragments arranged by size:
1186 297 281
NsiI A_TGCA'T
Cuts at: 0 965 1764
Size: 965 799
NspI r_CATG'y
Cuts at: 0 1579 1764
Size: 1579 185
PciI A'CATG_T
Cuts at: 0 1575 1764
Size: 1575 189
PmlI CAC'GTG
Cuts at: 0 981 1764
Size: 981 783
PspXI vC'TCGA_Gb
Cuts at: 0 7 1764
Size: 7 1757
PstI C_TGCA'G
Cuts at: 0 1561 1764
Size: 1561 203
PvuII CAG'CTG
Cuts at: 0 464 1764
Size: 464 1300
SalI G'TCGA_C
Cuts at: 0 1312 1764
Size: 1312 452
SapI GCTCTTCn'nnn_
Cuts at: 0 337 1764
Size: 337 1427
SfcI C'TryA_G
Cuts at: 0 1031 1073 1357 1557 1764
Size: 1031 42 284 200 207
Fragments arranged by size:
1031 284 207 200 42
SmlI C'TyrA_G
Cuts at: 0 7 1764
Size: 7 1757
SspI AAT'ATT
Cuts at: 0 85 1764
Size: 85 1679
StyI C'CwwG_G
Cuts at: 0 279 1186 1467 1764
Size: 279 907 281 297
Fragments arranged by size:
907 297 281 279
TatI w'GTAC_w
Cuts at: 0 37 1065 1543 1570 1764
Size: 37 1028 478 27 194
Fragments arranged by size:
1028 478 194 37 27
XhoI C'TCGA_G
Cuts at: 0 7 1764
Size: 7 1757
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflIII AlwNI ApoI AvaI
BanI BbsI BcgI BfrBI BglI BglII BmgBI BmrI
Bpu10I BsaAI BsaXI BseRI BseYI BsmBI BsrBI BsrGI
BstAPI BstYI BtgI BtsI DraI EarI EciI Eco57MI
EcoNI EcoRI EcoRV HaeII Hin4I HincII HindIII KpnI
MmeI MslI MspA1I NcoI NsiI NspI PciI PmlI
PspXI PstI PvuII SalI SapI SfcI SmlI SspI
StyI TatI XhoI
Enzymes that do not cut:
AarI AatII AclI AflII AgeI AhdI AleI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BanII BbeI BbvCI BciVI BclI BlpI Bme1580I
BmtI BplI BpmI BpuEI BsaI BsaBI BsaHI BsaWI
BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI
BspMI BsrDI BsrFI BssHII BssSI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI EaeI
EagI EcoICRI EcoO109I FalI FseI FspI FspAI HpaI
KasI MfeI MluI MscI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PasI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PsrI PvuI RsrII
SacI SacII SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
SwaI TaqII TstI Tth111I XbaI XcmI XmaI XmnI
ZraI