Restriction map for
pF1KB7893
(Linear) (Six-base) MAPSORT of: KIBB7893.seq Check: 207 from: 1 to: 1764
KIBB7893.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 264 1764
Size: 199 65 1500
Fragments arranged by size:
1500 199 65
ApaI G_GGCC'C
Cuts at: 0 73 1764
Size: 73 1691
ApaLI G'TGCA_C
Cuts at: 0 1388 1764
Size: 1388 376
ApoI r'AATT_y
Cuts at: 0 1135 1500 1764
Size: 1135 365 264
Fragments arranged by size:
1135 365 264
AvaI C'yCGr_G
Cuts at: 0 433 461 640 1088 1541 1764
Size: 433 28 179 448 453 223
Fragments arranged by size:
453 448 433 223 179 28
BanI G'GyrC_C
Cuts at: 0 94 613 1519 1764
Size: 94 519 906 245
Fragments arranged by size:
906 519 245 94
BanII G_rGCy'C
Cuts at: 0 40 64 73 326 434 647 701 1764
Size: 40 24 9 253 108 213 54 1063
Fragments arranged by size:
1063 253 213 108 54 40 24 9
BbeI G_GCGC'C
Cuts at: 0 617 1764
Size: 617 1147
BbvCI CC'TCA_GC
Cuts at: 0 152 1764
Size: 152 1612
BclI T'GATC_A
Cuts at: 0 1034 1764
Size: 1034 730
BfrBI ATG'CAT
Cuts at: 0 1259 1764
Size: 1259 505
BglI GCCn_nnn'nGGC
Cuts at: 0 68 77 1764
Size: 68 9 1687
Fragments arranged by size:
1687 68 9
Bme1580I G_kGCm'C
Cuts at: 0 73 137 1392 1764
Size: 73 64 1255 372
Fragments arranged by size:
1255 372 73 64
BmrI ACTGGGnnnn_n'
Cuts at: 0 68 1764
Size: 68 1696
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1575 1764
Size: 1575 189
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3 106 310 628 949 982 1015 1219
Size: 3 103 204 318 321 33 33 204
Cuts at: 1219 1545 1764
Size: 326 219
Fragments arranged by size:
326 321 318 219 204 204 103 33
33 3
Bpu10I CC'TnA_GC
Cuts at: 0 152 1294 1764
Size: 152 1142 470
Fragments arranged by size:
1142 470 152
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 823 1764
Size: 580 243 941
Fragments arranged by size:
941 580 243
BsaI GGTCTCn'nnnn_
Cuts at: 0 433 620 1480 1764
Size: 433 187 860 284
Fragments arranged by size:
860 433 284 187
BsaHI Gr'CG_yC
Cuts at: 0 614 1764
Size: 614 1150
BsaWI w'CCGG_w
Cuts at: 0 1392 1442 1764
Size: 1392 50 322
Fragments arranged by size:
1392 322 50
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 253 283 499 529 1522 1552 1764
Size: 253 30 216 30 993 30 212
Fragments arranged by size:
993 253 216 212 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 520 796 1548 1599 1764
Size: 520 276 752 51 165
Fragments arranged by size:
752 520 276 165 51
BseYI C'CCAG_C
Cuts at: 0 330 1664 1764
Size: 330 1334 100
Fragments arranged by size:
1334 330 100
BsiHKAI G_wGCw'C
Cuts at: 0 701 755 1392 1764
Size: 701 54 637 372
Fragments arranged by size:
701 637 372 54
BsiWI C'GTAC_G
Cuts at: 0 314 1764
Size: 314 1450
BsmI GAATG_Cn'
Cuts at: 0 1565 1764
Size: 1565 199
BsmBI CGTCTCn'nnnn_
Cuts at: 0 796 1469 1764
Size: 796 673 295
Fragments arranged by size:
796 673 295
Bsp1286I G_dGCh'C
Cuts at: 0 40 64 73 137 326 434 647 701
Size: 40 24 9 64 189 108 213 54
Cuts at: 701 755 1392 1764
Size: 54 637 372
Fragments arranged by size:
637 372 213 189 108 64 54 54
40 24 9
BspEI T'CCGG_A
Cuts at: 0 1442 1764
Size: 1442 322
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 730 1253 1595 1764
Size: 730 523 342 169
Fragments arranged by size:
730 523 342 169
BsrDI GCAATG_nn'
Cuts at: 0 222 1764
Size: 222 1542
BsrFI r'CCGG_y
Cuts at: 0 1712 1764
Size: 1712 52
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1698 1764
Size: 1698 66
BstEII G'GTnAC_C
Cuts at: 0 1336 1764
Size: 1336 428
BtgI C'CryG_G
Cuts at: 0 413 1764
Size: 413 1351
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1327 1764
Size: 1327 437
BtsI GCAGTG_nn'
Cuts at: 0 814 1764
Size: 814 950
DrdI GACnn_nn'nnGTC
Cuts at: 0 810 1591 1701 1764
Size: 810 781 110 63
Fragments arranged by size:
810 781 110 63
EaeI y'GGCC_r
Cuts at: 0 1470 1714 1764
Size: 1470 244 50
Fragments arranged by size:
1470 244 50
EarI CTCTTCn'nnn_
Cuts at: 0 706 1399 1764
Size: 706 693 365
Fragments arranged by size:
706 693 365
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 462 1303 1764
Size: 462 841 461
Fragments arranged by size:
841 462 461
EcoICRI GAG'CTC
Cuts at: 0 699 1764
Size: 699 1065
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3 106 199 264 310 628 949 982
Size: 3 103 93 65 46 318 321 33
Cuts at: 982 1015 1219 1545 1764
Size: 33 204 326 219
Fragments arranged by size:
326 321 318 219 204 103 93 65
46 33 33 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1198 1764
Size: 1198 566
EcoO109I rG'GnC_Cy
Cuts at: 0 70 945 957 1209 1764
Size: 70 875 12 252 555
Fragments arranged by size:
875 555 252 70 12
FspI TGC'GCA
Cuts at: 0 382 1764
Size: 382 1382
HaeII r_GCGC'y
Cuts at: 0 176 203 617 1764
Size: 176 27 414 1147
Fragments arranged by size:
1147 414 176 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 221 253 285 1764
Size: 221 32 32 1479
Fragments arranged by size:
1479 221 32 32
HincII GTy'rAC
Cuts at: 0 1728 1764
Size: 1728 36
KasI G'GCGC_C
Cuts at: 0 613 1764
Size: 613 1151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 1764
Size: 296 1468
MslI CAynn'nnrTG
Cuts at: 0 1346 1764
Size: 1346 418
MspA1I CmG'CkG
Cuts at: 0 151 330 636 831 1002 1098 1764
Size: 151 179 306 195 171 96 666
Fragments arranged by size:
666 306 195 179 171 151 96
NarI GG'CG_CC
Cuts at: 0 614 1764
Size: 614 1150
NsiI A_TGCA'T
Cuts at: 0 1261 1764
Size: 1261 503
NspI r_CATG'y
Cuts at: 0 744 1263 1764
Size: 744 519 501
Fragments arranged by size:
744 519 501
PfoI T'CCnGG_A
Cuts at: 0 19 403 650 746 926 941 1764
Size: 19 384 247 96 180 15 823
Fragments arranged by size:
823 384 247 180 96 19 15
PpuMI rG'GwC_Cy
Cuts at: 0 945 957 1764
Size: 945 12 807
Fragments arranged by size:
945 807 12
PspOMI G'GGCC_C
Cuts at: 0 69 1764
Size: 69 1695
PspXI vC'TCGA_Gb
Cuts at: 0 640 1764
Size: 640 1124
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 259 291 1764
Size: 259 32 1473
Fragments arranged by size:
1473 259 32
PstI C_TGCA'G
Cuts at: 0 12 836 1133 1764
Size: 12 824 297 631
Fragments arranged by size:
824 631 297 12
PvuII CAG'CTG
Cuts at: 0 151 330 636 831 1098 1764
Size: 151 179 306 195 267 666
Fragments arranged by size:
666 306 267 195 179 151
SacI G_AGCT'C
Cuts at: 0 701 1764
Size: 701 1063
SapI GCTCTTCn'nnn_
Cuts at: 0 706 1764
Size: 706 1058
ScaI AGT'ACT
Cuts at: 0 1555 1764
Size: 1555 209
SexAI A'CCwGG_T
Cuts at: 0 947 1764
Size: 947 817
SfcI C'TryA_G
Cuts at: 0 8 832 1129 1764
Size: 8 824 297 635
Fragments arranged by size:
824 635 297 8
SfoI GGC'GCC
Cuts at: 0 615 1764
Size: 615 1149
SmaI CCC'GGG
Cuts at: 0 435 463 1090 1543 1764
Size: 435 28 627 453 221
Fragments arranged by size:
627 453 435 221 28
SmlI C'TyrA_G
Cuts at: 0 595 640 838 1764
Size: 595 45 198 926
Fragments arranged by size:
926 595 198 45
SphI G_CATG'C
Cuts at: 0 1263 1764
Size: 1263 501
StuI AGG'CCT
Cuts at: 0 574 1764
Size: 574 1190
StyI C'CwwG_G
Cuts at: 0 98 788 1061 1481 1599 1658 1764
Size: 98 690 273 420 118 59 106
Fragments arranged by size:
690 420 273 118 106 98 59
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 727 1443 1507 1764
Size: 727 716 64 257
Fragments arranged by size:
727 716 257 64
TatI w'GTAC_w
Cuts at: 0 1553 1764
Size: 1553 211
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 499 531 1764
Size: 499 32 1233
Fragments arranged by size:
1233 499 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 81 1764
Size: 81 1683
XhoI C'TCGA_G
Cuts at: 0 640 1764
Size: 640 1124
XmaI C'CCGG_G
Cuts at: 0 433 461 1088 1541 1764
Size: 433 28 627 453 223
Fragments arranged by size:
627 453 433 223 28
XmnI GAAnn'nnTTC
Cuts at: 0 1369 1501 1764
Size: 1369 132 263
Fragments arranged by size:
1369 263 132
Enzymes that do cut:
AcuI ApaI ApaLI ApoI AvaI BanI BanII BbeI
BbvCI BclI BfrBI BglI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspMI BsrDI
BsrFI BstAPI BstEII BtgI BtgZI BtsI DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII
Hin4I HincII KasI MmeI MslI MspA1I NarI NsiI
NspI PfoI PpuMI PspOMI PspXI PsrI PstI PvuII
SacI SapI ScaI SexAI SfcI SfoI SmaI SmlI
SphI StuI StyI TaqII TatI TstI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BcgI BciVI BglII
BlpI BmgBI BmtI BsaAI BsaBI BsgI BsiEI BspHI
BsrBI BsrGI BssHII BssSI BstBI BstXI BstYI BstZ17I
Bsu36I ClaI CspCI DraI DraIII EagI EcoRI EcoRV
FalI FseI FspAI HindIII HpaI KpnI MfeI MluI
MscI NaeI NcoI NdeI NgoMIV NheI NotI NruI
PacI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PvuI RsrII SacII SalI SanDI SbfI SfiI
SgfI SgrAI SnaBI SpeI SrfI SspI SwaI Tth111I
XbaI ZraI