Restriction map for
pF1KB7880
(Linear) (Six-base) MAPSORT of: KIBB7880.seq Check: 6998 from: 1 to: 1920
KIBB7880.orf
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1534 1596 1920
Size: 1534 62 324
Fragments arranged by size:
1534 324 62
AfeI AGC'GCT
Cuts at: 0 14 1920
Size: 14 1906
AflII C'TTAA_G
Cuts at: 0 1465 1920
Size: 1465 455
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 517 1920
Size: 517 1403
AlwNI CAG_nnn'CTG
Cuts at: 0 1430 1920
Size: 1430 490
ApoI r'AATT_y
Cuts at: 0 521 887 1310 1394 1920
Size: 521 366 423 84 526
Fragments arranged by size:
526 521 423 366 84
BanI G'GyrC_C
Cuts at: 0 146 875 1889 1920
Size: 146 729 1014 31
Fragments arranged by size:
1014 729 146 31
BanII G_rGCy'C
Cuts at: 0 943 1919 1920
Size: 943 976 1
Fragments arranged by size:
976 943 1
BbsI GAAGACnn'nnnn_
Cuts at: 0 1602 1920
Size: 1602 318
BbvCI CC'TCA_GC
Cuts at: 0 221 1920
Size: 221 1699
BciVI GTATCCnnnnn_n'
Cuts at: 0 337 1920
Size: 337 1583
BclI T'GATC_A
Cuts at: 0 1215 1920
Size: 1215 705
Bme1580I G_kGCm'C
Cuts at: 0 878 1920
Size: 878 1042
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 746 1088 1172 1340 1424 1920
Size: 190 556 342 84 168 84 496
Fragments arranged by size:
556 496 342 190 168 84 84
Bpu10I CC'TnA_GC
Cuts at: 0 221 1920
Size: 221 1699
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 957 1318 1555 1654 1920
Size: 957 361 237 99 266
Fragments arranged by size:
957 361 266 237 99
BsaI GGTCTCn'nnnn_
Cuts at: 0 637 1920
Size: 637 1283
BsaBI GATnn'nnATC
Cuts at: 0 325 1920
Size: 325 1595
BsaWI w'CCGG_w
Cuts at: 0 1822 1920
Size: 1822 98
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 130 238 502 1920
Size: 130 108 264 1418
Fragments arranged by size:
1418 264 130 108
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1191 1695 1743 1920
Size: 1191 504 48 177
Fragments arranged by size:
1191 504 177 48
BsiHKAI G_wGCw'C
Cuts at: 0 943 1304 1640 1920
Size: 943 361 336 280
Fragments arranged by size:
943 361 336 280
BsmI GAATG_Cn'
Cuts at: 0 1127 1426 1510 1920
Size: 1127 299 84 410
Fragments arranged by size:
1127 410 299 84
Bsp1286I G_dGCh'C
Cuts at: 0 878 943 1304 1640 1919 1920
Size: 878 65 361 336 279 1
Fragments arranged by size:
878 361 336 279 65 1
BspHI T'CATG_A
Cuts at: 0 156 335 1920
Size: 156 179 1585
Fragments arranged by size:
1585 179 156
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 446 1920
Size: 446 1474
BsrBI CCG'CTC
Cuts at: 0 1367 1920
Size: 1367 553
BsrDI GCAATG_nn'
Cuts at: 0 1008 1599 1920
Size: 1008 591 321
Fragments arranged by size:
1008 591 321
BstXI CCAn_nnnn'nTGG
Cuts at: 0 463 1920
Size: 463 1457
BtgI C'CryG_G
Cuts at: 0 38 1920
Size: 38 1882
BtsI GCAGTG_nn'
Cuts at: 0 501 1179 1431 1515 1683 1760 1920
Size: 501 678 252 84 168 77 160
Fragments arranged by size:
678 501 252 168 160 84 77
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 819 854 990 1025 1245 1280 1581 1616
Size: 819 35 136 35 220 35 301 35
Cuts at: 1616 1920
Size: 304
Fragments arranged by size:
819 304 301 220 136 35 35 35
35
EaeI y'GGCC_r
Cuts at: 0 2 404 1044 1920
Size: 2 402 640 876
Fragments arranged by size:
876 640 402 2
EarI CTCTTCn'nnn_
Cuts at: 0 44 1907 1920
Size: 44 1863 13
Fragments arranged by size:
1863 44 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1012 1920
Size: 1012 908
EcoICRI GAG'CTC
Cuts at: 0 941 1920
Size: 941 979
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 746 1088 1172 1340 1424 1534 1596
Size: 190 556 342 84 168 84 110 62
Cuts at: 1596 1920
Size: 324
Fragments arranged by size:
556 342 324 190 168 110 84 84
62
EcoO109I rG'GnC_Cy
Cuts at: 0 123 228 289 1920
Size: 123 105 61 1631
Fragments arranged by size:
1631 123 105 61
EcoRI G'AATT_C
Cuts at: 0 887 1310 1394 1920
Size: 887 423 84 526
Fragments arranged by size:
887 526 423 84
FspI TGC'GCA
Cuts at: 0 254 1920
Size: 254 1666
HaeII r_GCGC'y
Cuts at: 0 16 1920
Size: 16 1904
HindIII A'AGCT_T
Cuts at: 0 734 1920
Size: 734 1186
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 598 618 1920
Size: 598 20 1302
Fragments arranged by size:
1302 598 20
MscI TGG'CCA
Cuts at: 0 406 1046 1920
Size: 406 640 874
Fragments arranged by size:
874 640 406
MslI CAynn'nnrTG
Cuts at: 0 314 1118 1920
Size: 314 804 802
Fragments arranged by size:
804 802 314
NcoI C'CATG_G
Cuts at: 0 38 1920
Size: 38 1882
NdeI CA'TA_TG
Cuts at: 0 1345 1920
Size: 1345 575
NspI r_CATG'y
Cuts at: 0 1383 1769 1920
Size: 1383 386 151
Fragments arranged by size:
1383 386 151
PpuMI rG'GwC_Cy
Cuts at: 0 289 1920
Size: 289 1631
PsiI TTA'TAA
Cuts at: 0 740 1754 1920
Size: 740 1014 166
Fragments arranged by size:
1014 740 166
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 760 792 1920
Size: 760 32 1128
Fragments arranged by size:
1128 760 32
SacI G_AGCT'C
Cuts at: 0 943 1920
Size: 943 977
SapI GCTCTTCn'nnn_
Cuts at: 0 1907 1920
Size: 1907 13
SmlI C'TyrA_G
Cuts at: 0 936 1297 1465 1534 1633 1920
Size: 936 361 168 69 99 287
Fragments arranged by size:
936 361 287 168 99 69
StuI AGG'CCT
Cuts at: 0 1077 1245 1273 1497 1749 1777 1920
Size: 1077 168 28 224 252 28 143
Fragments arranged by size:
1077 252 224 168 143 28 28
StyI C'CwwG_G
Cuts at: 0 38 1920
Size: 38 1882
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 622 1920
Size: 622 1298
TatI w'GTAC_w
Cuts at: 0 101 1920
Size: 101 1819
XmnI GAAnn'nnTTC
Cuts at: 0 847 1018 1189 1273 1441 1861 1920
Size: 847 171 171 84 168 420 59
Fragments arranged by size:
847 420 171 171 168 84 59
Enzymes that do cut:
AcuI AfeI AflII AlfI AlwNI ApoI BanI BanII
BbsI BbvCI BciVI BclI Bme1580I BpmI Bpu10I BpuEI
BsaI BsaBI BsaWI BseRI BsgI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BstXI BtgI BtsI CspCI
EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII HindIII MmeI MscI MslI NcoI NdeI NspI
PpuMI PsiI PsrI SacI SapI SmlI StuI StyI
TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflIII AgeI AhdI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BcgI BfrBI BglI BglII
BlpI BmgBI BmrI BmtI BplI BsaAI BsaHI BsaXI
BseYI BsiEI BsiWI BsmBI BspEI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI
ClaI DraI DraIII DrdI EagI EcoNI EcoRV FalI
FseI FspAI Hin4I HincII HpaI KasI KpnI MfeI
MluI MspA1I NaeI NarI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PspOMI PspXI PstI PvuI PvuII RsrII
SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TstI Tth111I XbaI XcmI XhoI XmaI
ZraI