Restriction map for
pF1KB7863
(Linear) (Six-base) MAPSORT of: KIBB7863.seq Check: 554 from: 1 to: 1662
KIBB7863.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 0 638 1662
Size: 638 1024
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 447 600 700 1265 1437 1662
Size: 346 101 153 100 565 172 225
Fragments arranged by size:
565 346 225 172 153 101 100
AflII C'TTAA_G
Cuts at: 0 481 1662
Size: 481 1181
AflIII A'CryG_T
Cuts at: 0 1540 1662
Size: 1540 122
AlwNI CAG_nnn'CTG
Cuts at: 0 10 1662
Size: 10 1652
ApoI r'AATT_y
Cuts at: 0 1391 1559 1655 1662
Size: 1391 168 96 7
Fragments arranged by size:
1391 168 96 7
AseI AT'TA_AT
Cuts at: 0 701 1662
Size: 701 961
AvaI C'yCGr_G
Cuts at: 0 1044 1662
Size: 1044 618
BbsI GAAGACnn'nnnn_
Cuts at: 0 453 1650 1662
Size: 453 1197 12
Fragments arranged by size:
1197 453 12
BciVI GTATCCnnnnn_n'
Cuts at: 0 392 1301 1662
Size: 392 909 361
Fragments arranged by size:
909 392 361
BfrBI ATG'CAT
Cuts at: 0 1059 1662
Size: 1059 603
Bme1580I G_kGCm'C
Cuts at: 0 1332 1662
Size: 1332 330
BmgBI CAC'GTC
Cuts at: 0 207 1662
Size: 207 1455
BmrI ACTGGGnnnn_n'
Cuts at: 0 40 257 342 917 1662
Size: 40 217 85 575 745
Fragments arranged by size:
745 575 217 85 40
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 334 585 766 1662
Size: 334 251 181 896
Fragments arranged by size:
896 334 251 181
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 1013 1181 1433 1517 1662
Size: 235 778 168 252 84 145
Fragments arranged by size:
778 252 235 168 145 84
BsaAI yAC'GTr
Cuts at: 0 492 1541 1662
Size: 492 1049 121
Fragments arranged by size:
1049 492 121
BsaBI GATnn'nnATC
Cuts at: 0 110 1662
Size: 110 1552
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 175 326 1662
Size: 175 151 1336
Fragments arranged by size:
1336 175 151
BseYI C'CCAG_C
Cuts at: 0 873 1662
Size: 873 789
BsmI GAATG_Cn'
Cuts at: 0 1519 1662
Size: 1519 143
Bsp1286I G_dGCh'C
Cuts at: 0 1332 1662
Size: 1332 330
BspHI T'CATG_A
Cuts at: 0 57 519 1563 1662
Size: 57 462 1044 99
Fragments arranged by size:
1044 462 99 57
BsrDI GCAATG_nn'
Cuts at: 0 454 1612 1662
Size: 454 1158 50
Fragments arranged by size:
1158 454 50
BstBI TT'CG_AA
Cuts at: 0 1641 1662
Size: 1641 21
BstZ17I GTA'TAC
Cuts at: 0 639 1662
Size: 639 1023
BtgI C'CryG_G
Cuts at: 0 21 1662
Size: 21 1641
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1506 1541 1662
Size: 1506 35 121
Fragments arranged by size:
1506 121 35
EaeI y'GGCC_r
Cuts at: 0 233 1662
Size: 233 1429
EarI CTCTTCn'nnn_
Cuts at: 0 284 1241 1577 1662
Size: 284 957 336 85
Fragments arranged by size:
957 336 284 85
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 346 447 600 700 1013 1181 1265
Size: 235 111 101 153 100 313 168 84
Cuts at: 1265 1433 1437 1517 1662
Size: 168 4 80 145
Fragments arranged by size:
313 235 168 168 153 145 111 101
100 84 80 4
EcoO109I rG'GnC_Cy
Cuts at: 0 42 1662
Size: 42 1620
EcoRI G'AATT_C
Cuts at: 0 1559 1662
Size: 1559 103
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 565 1662
Size: 565 1097
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 236 268 1662
Size: 236 32 1394
Fragments arranged by size:
1394 236 32
HindIII A'AGCT_T
Cuts at: 0 1616 1662
Size: 1616 46
MfeI C'AATT_G
Cuts at: 0 403 1662
Size: 403 1259
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 983 1662
Size: 260 723 679
Fragments arranged by size:
723 679 260
MscI TGG'CCA
Cuts at: 0 235 1662
Size: 235 1427
MslI CAynn'nnrTG
Cuts at: 0 672 924 1662
Size: 672 252 738
Fragments arranged by size:
738 672 252
MspA1I CmG'CkG
Cuts at: 0 7 1662
Size: 7 1655
NcoI C'CATG_G
Cuts at: 0 21 1662
Size: 21 1641
NdeI CA'TA_TG
Cuts at: 0 725 921 1662
Size: 725 196 741
Fragments arranged by size:
741 725 196
NsiI A_TGCA'T
Cuts at: 0 1061 1662
Size: 1061 601
NspI r_CATG'y
Cuts at: 0 897 1063 1317 1401 1662
Size: 897 166 254 84 261
Fragments arranged by size:
897 261 254 166 84
PmlI CAC'GTG
Cuts at: 0 492 1541 1662
Size: 492 1049 121
Fragments arranged by size:
1049 492 121
PpuMI rG'GwC_Cy
Cuts at: 0 42 1662
Size: 42 1620
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 489 521 1662
Size: 489 32 1141
Fragments arranged by size:
1141 489 32
PvuII CAG'CTG
Cuts at: 0 7 1662
Size: 7 1655
SanDI GG'GwC_CC
Cuts at: 0 42 1662
Size: 42 1620
ScaI AGT'ACT
Cuts at: 0 1042 1662
Size: 1042 620
SfcI C'TryA_G
Cuts at: 0 198 864 1662
Size: 198 666 798
Fragments arranged by size:
798 666 198
SmlI C'TyrA_G
Cuts at: 0 481 1662
Size: 481 1181
SspI AAT'ATT
Cuts at: 0 633 1662
Size: 633 1029
StuI AGG'CCT
Cuts at: 0 1255 1450 1662
Size: 1255 195 212
Fragments arranged by size:
1255 212 195
StyI C'CwwG_G
Cuts at: 0 21 321 1662
Size: 21 300 1341
Fragments arranged by size:
1341 300 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 169 1396 1662
Size: 169 1227 266
Fragments arranged by size:
1227 266 169
TatI w'GTAC_w
Cuts at: 0 505 1040 1662
Size: 505 535 622
Fragments arranged by size:
622 535 505
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 71 103 1662
Size: 71 32 1559
Fragments arranged by size:
1559 71 32
XmnI GAAnn'nnTTC
Cuts at: 0 946 1030 1114 1534 1655 1662
Size: 946 84 84 420 121 7
Fragments arranged by size:
946 420 121 84 84 7
Enzymes that do cut:
AccI AcuI AflII AflIII AlwNI ApoI AseI AvaI
BbsI BciVI BfrBI Bme1580I BmgBI BmrI BplI BpmI
BsaAI BsaBI BseRI BseYI BsmI Bsp1286I BspHI BsrDI
BstBI BstZ17I BtgI CspCI EaeI EarI Eco57MI EcoO109I
EcoRI FalI Hin4I HindIII MfeI MmeI MscI MslI
MspA1I NcoI NdeI NsiI NspI PmlI PpuMI PsrI
PvuII SanDI ScaI SfcI SmlI SspI StuI StyI
TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AsiSI AvrII BaeI
BamHI BanI BanII BbeI BbvCI BcgI BclI BglI
BglII BlpI BmtI Bpu10I BpuEI BsaI BsaHI BsaWI
BsaXI BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI BspMI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgZI BtsI ClaI DraI DraIII DrdI
EagI EciI EcoICRI EcoNI EcoRV FseI FspI FspAI
HaeII HincII HpaI KasI KpnI MluI NaeI NarI
NgoMIV NheI NotI NruI PacI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PstI
PvuI RsrII SacI SacII SalI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SwaI Tth111I XbaI XcmI XhoI XmaI ZraI