Restriction map for
pF1KB7838
(Linear) (Six-base) MAPSORT of: KIBB7838.seq Check: 8551 from: 1 to: 1842
KIBB7838.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1020 1212 1842
Size: 1020 192 630
Fragments arranged by size:
1020 630 192
AccI GT'mk_AC
Cuts at: 0 306 1842
Size: 306 1536
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 529 1551 1744 1842
Size: 529 1022 193 98
Fragments arranged by size:
1022 529 193 98
AflII C'TTAA_G
Cuts at: 0 1414 1842
Size: 1414 428
AflIII A'CryG_T
Cuts at: 0 1175 1262 1842
Size: 1175 87 580
Fragments arranged by size:
1175 580 87
AleI CACnn'nnGTG
Cuts at: 0 431 1174 1624 1842
Size: 431 743 450 218
Fragments arranged by size:
743 450 431 218
AlwNI CAG_nnn'CTG
Cuts at: 0 129 1842
Size: 129 1713
ApaI G_GGCC'C
Cuts at: 0 754 885 1842
Size: 754 131 957
Fragments arranged by size:
957 754 131
ApaLI G'TGCA_C
Cuts at: 0 436 1842
Size: 436 1406
ApoI r'AATT_y
Cuts at: 0 1662 1842
Size: 1662 180
AvaI C'yCGr_G
Cuts at: 0 417 596 878 1371 1650 1842
Size: 417 179 282 493 279 192
Fragments arranged by size:
493 417 282 279 192 179
BanI G'GyrC_C
Cuts at: 0 1154 1200 1423 1525 1842
Size: 1154 46 223 102 317
Fragments arranged by size:
1154 317 223 102 46
BanII G_rGCy'C
Cuts at: 0 295 754 848 885 1707 1716 1842
Size: 295 459 94 37 822 9 126
Fragments arranged by size:
822 459 295 126 94 37 9
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1631 1665 1842
Size: 1631 34 177
Fragments arranged by size:
1631 177 34
BfrBI ATG'CAT
Cuts at: 0 955 1803 1842
Size: 955 848 39
Fragments arranged by size:
955 848 39
BglI GCCn_nnn'nGGC
Cuts at: 0 749 1842
Size: 749 1093
BglII A'GATC_T
Cuts at: 0 893 1301 1842
Size: 893 408 541
Fragments arranged by size:
893 541 408
Bme1580I G_kGCm'C
Cuts at: 0 440 706 754 885 1157 1842
Size: 440 266 48 131 272 685
Fragments arranged by size:
685 440 272 266 131 48
BmrI ACTGGGnnnn_n'
Cuts at: 0 795 1842
Size: 795 1047
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 540 1332 1564 1842
Size: 540 792 232 278
Fragments arranged by size:
792 540 278 232
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 730 953 1669 1842
Size: 39 691 223 716 173
Fragments arranged by size:
716 691 223 173 39
Bpu10I CC'TnA_GC
Cuts at: 0 842 1492 1842
Size: 842 650 350
Fragments arranged by size:
842 650 350
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1753 1842
Size: 1753 89
BsaI GGTCTCn'nnnn_
Cuts at: 0 1535 1842
Size: 1535 307
BsaAI yAC'GTr
Cuts at: 0 1176 1842
Size: 1176 666
BsaHI Gr'CG_yC
Cuts at: 0 35 820 1835 1842
Size: 35 785 1015 7
Fragments arranged by size:
1015 785 35 7
BsaWI w'CCGG_w
Cuts at: 0 771 1842
Size: 771 1071
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 37 67 1092 1122 1842
Size: 37 30 1025 30 720
Fragments arranged by size:
1025 720 37 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 54 261 264 1077 1615 1618 1842
Size: 54 207 3 813 538 3 224
Fragments arranged by size:
813 538 224 207 54 3 3
BseYI C'CCAG_C
Cuts at: 0 217 343 725 918 989 1043 1608 1842
Size: 217 126 382 193 71 54 565 234
Fragments arranged by size:
565 382 234 217 193 126 71 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1103 1315 1842
Size: 1103 212 527
Fragments arranged by size:
1103 527 212
BsiHKAI G_wGCw'C
Cuts at: 0 355 440 1842
Size: 355 85 1402
Fragments arranged by size:
1402 355 85
BsmI GAATG_Cn'
Cuts at: 0 209 1803 1842
Size: 209 1594 39
Fragments arranged by size:
1594 209 39
BsmBI CGTCTCn'nnnn_
Cuts at: 0 593 1684 1842
Size: 593 1091 158
Fragments arranged by size:
1091 593 158
Bsp1286I G_dGCh'C
Cuts at: 0 295 355 440 706 754 848 885 1157
Size: 295 60 85 266 48 94 37 272
Cuts at: 1157 1707 1716 1842
Size: 550 9 126
Fragments arranged by size:
550 295 272 266 126 94 85 60
48 37 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1020 1212 1553 1790 1842
Size: 1020 192 341 237 52
Fragments arranged by size:
1020 341 237 192 52
BsrBI CCG'CTC
Cuts at: 0 1649 1842
Size: 1649 193
BsrDI GCAATG_nn'
Cuts at: 0 565 1842
Size: 565 1277
BsrFI r'CCGG_y
Cuts at: 0 1730 1842
Size: 1730 112
BstAPI GCAn_nnn'nTGC
Cuts at: 0 551 645 701 1842
Size: 551 94 56 1141
Fragments arranged by size:
1141 551 94 56
BstXI CCAn_nnnn'nTGG
Cuts at: 0 675 989 1536 1842
Size: 675 314 547 306
Fragments arranged by size:
675 547 314 306
BstYI r'GATC_y
Cuts at: 0 829 893 1301 1842
Size: 829 64 408 541
Fragments arranged by size:
829 541 408 64
BtgI C'CryG_G
Cuts at: 0 317 746 1529 1622 1842
Size: 317 429 783 93 220
Fragments arranged by size:
783 429 317 220 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 970 1842
Size: 970 872
BtsI GCAGTG_nn'
Cuts at: 0 1287 1842
Size: 1287 555
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 12 47 1842
Size: 12 35 1795
Fragments arranged by size:
1795 35 12
EaeI y'GGCC_r
Cuts at: 0 314 677 1842
Size: 314 363 1165
Fragments arranged by size:
1165 363 314
EarI CTCTTCn'nnn_
Cuts at: 0 1621 1712 1842
Size: 1621 91 130
Fragments arranged by size:
1621 130 91
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 529 730 953 1551 1669 1744 1842
Size: 39 490 201 223 598 118 75 98
Fragments arranged by size:
598 490 223 201 118 98 75 39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1414 1842
Size: 1414 428
EcoO109I rG'GnC_Cy
Cuts at: 0 742 751 1059 1842
Size: 742 9 308 783
Fragments arranged by size:
783 742 308 9
EcoRI G'AATT_C
Cuts at: 0 1662 1842
Size: 1662 180
EcoRV GAT'ATC
Cuts at: 0 866 1842
Size: 866 976
HaeII r_GCGC'y
Cuts at: 0 1594 1842
Size: 1594 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 220 252 400 432 850 882 1842
Size: 220 32 148 32 418 32 960
Fragments arranged by size:
960 418 220 148 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 451 1166 1842
Size: 41 410 715 676
Fragments arranged by size:
715 676 410 41
MscI TGG'CCA
Cuts at: 0 316 679 1842
Size: 316 363 1163
Fragments arranged by size:
1163 363 316
MslI CAynn'nnrTG
Cuts at: 0 431 1174 1178 1534 1624 1842
Size: 431 743 4 356 90 218
Fragments arranged by size:
743 431 356 218 90 4
MspA1I CmG'CkG
Cuts at: 0 551 642 708 816 1130 1336 1624 1842
Size: 551 91 66 108 314 206 288 218
Fragments arranged by size:
551 314 288 218 206 108 91 66
NcoI C'CATG_G
Cuts at: 0 317 746 1529 1842
Size: 317 429 783 313
Fragments arranged by size:
783 429 317 313
NsiI A_TGCA'T
Cuts at: 0 957 1805 1842
Size: 957 848 37
Fragments arranged by size:
957 848 37
NspI r_CATG'y
Cuts at: 0 594 1253 1266 1803 1842
Size: 594 659 13 537 39
Fragments arranged by size:
659 594 537 39 13
PasI CC'CwG_GG
Cuts at: 0 298 1842
Size: 298 1544
PciI A'CATG_T
Cuts at: 0 1262 1842
Size: 1262 580
PfoI T'CCnGG_A
Cuts at: 0 1685 1842
Size: 1685 157
PmlI CAC'GTG
Cuts at: 0 1176 1842
Size: 1176 666
PspOMI G'GGCC_C
Cuts at: 0 750 881 1842
Size: 750 131 961
Fragments arranged by size:
961 750 131
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 478 510 1842
Size: 478 32 1332
Fragments arranged by size:
1332 478 32
PstI C_TGCA'G
Cuts at: 0 1517 1644 1842
Size: 1517 127 198
Fragments arranged by size:
1517 198 127
PvuII CAG'CTG
Cuts at: 0 816 1130 1842
Size: 816 314 712
Fragments arranged by size:
816 712 314
SacII CC_GC'GG
Cuts at: 0 1625 1842
Size: 1625 217
SapI GCTCTTCn'nnn_
Cuts at: 0 1712 1842
Size: 1712 130
SbfI CC_TGCA'GG
Cuts at: 0 1517 1842
Size: 1517 325
SfcI C'TryA_G
Cuts at: 0 96 1506 1513 1640 1842
Size: 96 1410 7 127 202
Fragments arranged by size:
1410 202 127 96 7
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 749 1842
Size: 749 1093
SmaI CCC'GGG
Cuts at: 0 880 1373 1842
Size: 880 493 469
Fragments arranged by size:
880 493 469
SmlI C'TyrA_G
Cuts at: 0 596 1414 1768 1842
Size: 596 818 354 74
Fragments arranged by size:
818 596 354 74
SrfI GCCC'GGGC
Cuts at: 0 880 1842
Size: 880 962
StyI C'CwwG_G
Cuts at: 0 135 317 746 1034 1529 1736 1842
Size: 135 182 429 288 495 207 106
Fragments arranged by size:
495 429 288 207 182 135 106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 196 231 735 739 1186 1842
Size: 196 35 504 4 447 656
Fragments arranged by size:
656 504 447 196 35 4
TatI w'GTAC_w
Cuts at: 0 494 1842
Size: 494 1348
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1092 1124 1842
Size: 1092 32 718
Fragments arranged by size:
1092 718 32
Tth111I GACn'n_nGTC
Cuts at: 0 1402 1842
Size: 1402 440
XhoI C'TCGA_G
Cuts at: 0 596 1842
Size: 596 1246
XmaI C'CCGG_G
Cuts at: 0 878 1371 1842
Size: 878 493 471
Fragments arranged by size:
878 493 471
Enzymes that do cut:
AarI AccI AcuI AflII AflIII AleI AlwNI ApaI
ApaLI ApoI AvaI BanI BanII BcgI BfrBI BglI
BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI
BstXI BstYI BtgI BtgZI BtsI CspCI EaeI EarI
Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I MmeI
MscI MslI MspA1I NcoI NsiI NspI PasI PciI
PfoI PmlI PspOMI PsrI PstI PvuII SacII SapI
SbfI SfcI SfiI SmaI SmlI SrfI StyI TaqII
TatI TstI Tth111I XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AgeI AhdI AlfI AloI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI
BbvCI BciVI BclI BlpI BmgBI BmtI BsaBI BsiEI
BsiWI BspEI BspHI BsrGI BssHII BssSI BstBI BstEII
BstZ17I Bsu36I ClaI DraI DraIII DrdI EagI EciI
EcoICRI FalI FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PflMI PmeI PpiI PpuMI
PshAI PsiI PspXI PvuI RsrII SacI SalI SanDI
ScaI SexAI SfoI SgfI SgrAI SnaBI SpeI SphI
SspI StuI SwaI XbaI XcmI XmnI ZraI