Restriction map for
pF1KB7832
(Linear) (Six-base) MAPSORT of: KIBB7832.seq Check: 5885 from: 1 to: 1830
KIBB7832.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AflIII A'CryG_T
Cuts at: 0 916 1830
Size: 916 914
AleI CACnn'nnGTG
Cuts at: 0 17 1001 1830
Size: 17 984 829
Fragments arranged by size:
984 829 17
AlwNI CAG_nnn'CTG
Cuts at: 0 225 1830
Size: 225 1605
ApaLI G'TGCA_C
Cuts at: 0 751 1830
Size: 751 1079
ApoI r'AATT_y
Cuts at: 0 94 281 1515 1536 1830
Size: 94 187 1234 21 294
Fragments arranged by size:
1234 294 187 94 21
AvaI C'yCGr_G
Cuts at: 0 703 1830
Size: 703 1127
BanII G_rGCy'C
Cuts at: 0 1457 1830
Size: 1457 373
BbsI GAAGACnn'nnnn_
Cuts at: 0 421 1113 1830
Size: 421 692 717
Fragments arranged by size:
717 692 421
BciVI GTATCCnnnnn_n'
Cuts at: 0 1734 1830
Size: 1734 96
BclI T'GATC_A
Cuts at: 0 189 1830
Size: 189 1641
BfrBI ATG'CAT
Cuts at: 0 619 1137 1830
Size: 619 518 693
Fragments arranged by size:
693 619 518
Bme1580I G_kGCm'C
Cuts at: 0 755 1830
Size: 755 1075
BmrI ACTGGGnnnn_n'
Cuts at: 0 593 1830
Size: 593 1237
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1289 1830
Size: 1289 541
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1334 1575 1830
Size: 1334 241 255
Fragments arranged by size:
1334 255 241
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1189 1259 1830
Size: 1189 70 571
Fragments arranged by size:
1189 571 70
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1734 1764 1830
Size: 1734 30 66
Fragments arranged by size:
1734 66 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1592 1616 1830
Size: 1592 24 214
Fragments arranged by size:
1592 214 24
BsiHKAI G_wGCw'C
Cuts at: 0 26 755 804 1457 1830
Size: 26 729 49 653 373
Fragments arranged by size:
729 653 373 49 26
BsmI GAATG_Cn'
Cuts at: 0 621 1830
Size: 621 1209
Bsp1286I G_dGCh'C
Cuts at: 0 26 755 804 1457 1830
Size: 26 729 49 653 373
Fragments arranged by size:
729 653 373 49 26
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 49 1830
Size: 49 1781
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1003 1830
Size: 1003 827
Bsu36I CC'TnA_GG
Cuts at: 0 1446 1830
Size: 1446 384
DraI TTT'AAA
Cuts at: 0 105 254 1541 1830
Size: 105 149 1287 289
Fragments arranged by size:
1287 289 149 105
EarI CTCTTCn'nnn_
Cuts at: 0 308 866 1700 1830
Size: 308 558 834 130
Fragments arranged by size:
834 558 308 130
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 871 1830
Size: 871 959
EcoICRI GAG'CTC
Cuts at: 0 1455 1830
Size: 1455 375
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1334 1575 1830
Size: 1334 241 255
Fragments arranged by size:
1334 255 241
EcoNI CCTnn'n_nnAGG
Cuts at: 0 503 1830
Size: 503 1327
EcoRI G'AATT_C
Cuts at: 0 94 1830
Size: 94 1736
EcoRV GAT'ATC
Cuts at: 0 386 1830
Size: 386 1444
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 604 636 697 729 1481 1513 1732 1764
Size: 604 32 61 32 752 32 219 32
Cuts at: 1764 1830
Size: 66
Fragments arranged by size:
752 604 219 66 61 32 32 32
32
HincII GTy'rAC
Cuts at: 0 710 1778 1830
Size: 710 1068 52
Fragments arranged by size:
1068 710 52
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 247 1830
Size: 209 38 1583
Fragments arranged by size:
1583 209 38
MslI CAynn'nnrTG
Cuts at: 0 17 116 1001 1058 1074 1142 1397 1413
Size: 17 99 885 57 16 68 255 16
Cuts at: 1413 1568 1830
Size: 155 262
Fragments arranged by size:
885 262 255 155 99 68 57 17
16 16
MspA1I CmG'CkG
Cuts at: 0 51 1032 1830
Size: 51 981 798
Fragments arranged by size:
981 798 51
NdeI CA'TA_TG
Cuts at: 0 1242 1414 1830
Size: 1242 172 416
Fragments arranged by size:
1242 416 172
NsiI A_TGCA'T
Cuts at: 0 621 1139 1830
Size: 621 518 691
Fragments arranged by size:
691 621 518
NspI r_CATG'y
Cuts at: 0 920 1045 1137 1725 1830
Size: 920 125 92 588 105
Fragments arranged by size:
920 588 125 105 92
PciI A'CATG_T
Cuts at: 0 916 1830
Size: 916 914
PsiI TTA'TAA
Cuts at: 0 449 1830
Size: 449 1381
PspXI vC'TCGA_Gb
Cuts at: 0 703 1830
Size: 703 1127
PvuII CAG'CTG
Cuts at: 0 1032 1830
Size: 1032 798
SacI G_AGCT'C
Cuts at: 0 1457 1830
Size: 1457 373
SapI GCTCTTCn'nnn_
Cuts at: 0 866 1700 1830
Size: 866 834 130
Fragments arranged by size:
866 834 130
SfcI C'TryA_G
Cuts at: 0 156 377 476 662 1830
Size: 156 221 99 186 1168
Fragments arranged by size:
1168 221 186 156 99
SmlI C'TyrA_G
Cuts at: 0 703 1204 1274 1830
Size: 703 501 70 556
Fragments arranged by size:
703 556 501 70
SphI G_CATG'C
Cuts at: 0 1137 1830
Size: 1137 693
StyI C'CwwG_G
Cuts at: 0 893 1830
Size: 893 937
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1460 1830
Size: 1460 370
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1212 1244 1456 1488 1830
Size: 1212 32 212 32 342
Fragments arranged by size:
1212 342 212 32 32
XbaI T'CTAG_A
Cuts at: 0 1584 1830
Size: 1584 246
XhoI C'TCGA_G
Cuts at: 0 703 1830
Size: 703 1127
XmnI GAAnn'nnTTC
Cuts at: 0 165 553 1830
Size: 165 388 1277
Fragments arranged by size:
1277 388 165
Enzymes that do cut:
AflIII AleI AlwNI ApaLI ApoI AvaI BanII BbsI
BciVI BclI BfrBI Bme1580I BmrI BplI BpmI BpuEI
BsaXI BsgI BsiHKAI BsmI Bsp1286I BspMI BstXI Bsu36I
DraI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV
Hin4I HincII MmeI MslI MspA1I NdeI NsiI NspI
PciI PsiI PspXI PvuII SacI SapI SfcI SmlI
SphI StyI TaqII TstI XbaI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AlfI AloI ApaI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BglI
BglII BlpI BmgBI BmtI Bpu10I BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiWI BsmBI BspEI
BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraIII DrdI EaeI EagI EcoO109I FalI FseI
FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NcoI NgoMIV NheI NotI
NruI PacI PasI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PsrI PstI PvuI RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI
TatI Tth111I XcmI XmaI ZraI