Restriction map for
pF1KB7807
(Linear) (Six-base) MAPSORT of: KIBB7807.seq Check: 529 from: 1 to: 1575
KIBB7807.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 670 1575
Size: 670 905
Acc65I G'GTAC_C
Cuts at: 0 403 1575
Size: 403 1172
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 321 1121 1144 1575
Size: 321 800 23 431
Fragments arranged by size:
800 431 321 23
AlwNI CAG_nnn'CTG
Cuts at: 0 213 352 1189 1374 1439 1575
Size: 213 139 837 185 65 136
Fragments arranged by size:
837 213 185 139 136 65
AvaI C'yCGr_G
Cuts at: 0 92 1052 1575
Size: 92 960 523
Fragments arranged by size:
960 523 92
BanI G'GyrC_C
Cuts at: 0 290 403 1575
Size: 290 113 1172
Fragments arranged by size:
1172 290 113
BanII G_rGCy'C
Cuts at: 0 335 487 959 1028 1087 1575
Size: 335 152 472 69 59 488
Fragments arranged by size:
488 472 335 152 69 59
BbeI G_GCGC'C
Cuts at: 0 294 1575
Size: 294 1281
BbvCI CC'TCA_GC
Cuts at: 0 1432 1559 1575
Size: 1432 127 16
Fragments arranged by size:
1432 127 16
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 958 992 1575
Size: 958 34 583
Fragments arranged by size:
958 583 34
BglII A'GATC_T
Cuts at: 0 386 1575
Size: 386 1189
BlpI GC'TnA_GC
Cuts at: 0 943 1575
Size: 943 632
Bme1580I G_kGCm'C
Cuts at: 0 93 465 839 1575
Size: 93 372 374 736
Fragments arranged by size:
736 374 372 93
BmgBI CAC'GTC
Cuts at: 0 204 1575
Size: 204 1371
BmrI ACTGGGnnnn_n'
Cuts at: 0 80 668 698 975 1117 1575
Size: 80 588 30 277 142 458
Fragments arranged by size:
588 458 277 142 80 30
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 627 1042 1047 1575
Size: 627 415 5 528
Fragments arranged by size:
627 528 415 5
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 351 554 730 1071 1243 1575
Size: 262 89 203 176 341 172 332
Fragments arranged by size:
341 332 262 203 176 172 89
Bpu10I CC'TnA_GC
Cuts at: 0 1432 1559 1575
Size: 1432 127 16
Fragments arranged by size:
1432 127 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 1575
Size: 64 1511
BsaI GGTCTCn'nnnn_
Cuts at: 0 169 1575
Size: 169 1406
BsaHI Gr'CG_yC
Cuts at: 0 291 1300 1575
Size: 291 1009 275
Fragments arranged by size:
1009 291 275
BsaWI w'CCGG_w
Cuts at: 0 758 1575
Size: 758 817
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1057 1087 1575
Size: 1057 30 488
Fragments arranged by size:
1057 488 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 244 247 608 616 656 892 1240 1575
Size: 244 3 361 8 40 236 348 335
Fragments arranged by size:
361 348 335 244 236 40 8 3
BseYI C'CCAG_C
Cuts at: 0 117 664 781 1216 1575
Size: 117 547 117 435 359
Fragments arranged by size:
547 435 359 117 117
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 934 1575
Size: 226 708 641
Fragments arranged by size:
708 641 226
BsiEI CG_ry'CG
Cuts at: 0 1419 1575
Size: 1419 156
BsiHKAI G_wGCw'C
Cuts at: 0 568 959 1028 1063 1575
Size: 568 391 69 35 512
Fragments arranged by size:
568 512 391 69 35
BsmBI CGTCTCn'nnnn_
Cuts at: 0 369 1413 1575
Size: 369 1044 162
Fragments arranged by size:
1044 369 162
Bsp1286I G_dGCh'C
Cuts at: 0 93 335 465 487 568 839 959 1028
Size: 93 242 130 22 81 271 120 69
Cuts at: 1028 1063 1087 1575
Size: 35 24 488
Fragments arranged by size:
488 271 242 130 120 93 81 69
35 24 22
BspEI T'CCGG_A
Cuts at: 0 758 1575
Size: 758 817
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 670 1325 1575
Size: 670 655 250
Fragments arranged by size:
670 655 250
BsrBI CCG'CTC
Cuts at: 0 378 1575
Size: 378 1197
BsrDI GCAATG_nn'
Cuts at: 0 432 1327 1575
Size: 432 895 248
Fragments arranged by size:
895 432 248
BsrFI r'CCGG_y
Cuts at: 0 1568 1575
Size: 1568 7
BssHII G'CGCG_C
Cuts at: 0 150 1575
Size: 150 1425
BssSI C'ACGA_G
Cuts at: 0 952 1575
Size: 952 623
BstAPI GCAn_nnn'nTGC
Cuts at: 0 460 1575
Size: 460 1115
BstYI r'GATC_y
Cuts at: 0 386 548 752 881 1575
Size: 386 162 204 129 694
Fragments arranged by size:
694 386 204 162 129
Bsu36I CC'TnA_GG
Cuts at: 0 650 1575
Size: 650 925
BtgI C'CryG_G
Cuts at: 0 98 1338 1575
Size: 98 1240 237
Fragments arranged by size:
1240 237 98
BtsI GCAGTG_nn'
Cuts at: 0 103 465 514 1575
Size: 103 362 49 1061
Fragments arranged by size:
1061 362 103 49
EaeI y'GGCC_r
Cuts at: 0 266 553 906 1575
Size: 266 287 353 669
Fragments arranged by size:
669 353 287 266
EarI CTCTTCn'nnn_
Cuts at: 0 340 385 593 869 1033 1575
Size: 340 45 208 276 164 542
Fragments arranged by size:
542 340 276 208 164 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 56 1575
Size: 56 1519
EcoICRI GAG'CTC
Cuts at: 0 957 1026 1575
Size: 957 69 549
Fragments arranged by size:
957 549 69
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 321 351 554 730 1071 1121 1144
Size: 262 59 30 203 176 341 50 23
Cuts at: 1144 1243 1575
Size: 99 332
Fragments arranged by size:
341 332 262 203 176 99 59 50
30 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 298 533 1181 1225 1502 1575
Size: 298 235 648 44 277 73
Fragments arranged by size:
648 298 277 235 73 44
EcoO109I rG'GnC_Cy
Cuts at: 0 141 526 540 1114 1186 1507 1575
Size: 141 385 14 574 72 321 68
Fragments arranged by size:
574 385 321 141 72 68 14
HaeII r_GCGC'y
Cuts at: 0 13 56 294 1238 1575
Size: 13 43 238 944 337
Fragments arranged by size:
944 337 238 43 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 493 525 778 810 1575
Size: 493 32 253 32 765
Fragments arranged by size:
765 493 253 32 32
HincII GTy'rAC
Cuts at: 0 343 1575
Size: 343 1232
KasI G'GCGC_C
Cuts at: 0 290 1575
Size: 290 1285
KpnI G_GTAC'C
Cuts at: 0 407 1575
Size: 407 1168
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 557 1125 1575
Size: 557 568 450
Fragments arranged by size:
568 557 450
MscI TGG'CCA
Cuts at: 0 268 555 1575
Size: 268 287 1020
Fragments arranged by size:
1020 287 268
MslI CAynn'nnrTG
Cuts at: 0 733 1575
Size: 733 842
MspA1I CmG'CkG
Cuts at: 0 132 213 1575
Size: 132 81 1362
Fragments arranged by size:
1362 132 81
NaeI GCC'GGC
Cuts at: 0 1570 1575
Size: 1570 5
NarI GG'CG_CC
Cuts at: 0 291 1575
Size: 291 1284
NcoI C'CATG_G
Cuts at: 0 98 1338 1575
Size: 98 1240 237
Fragments arranged by size:
1240 237 98
NgoMIV G'CCGG_C
Cuts at: 0 1568 1575
Size: 1568 7
PasI CC'CwG_GG
Cuts at: 0 442 1575
Size: 442 1133
PflMI CCAn_nnn'nTGG
Cuts at: 0 520 1339 1575
Size: 520 819 236
Fragments arranged by size:
819 520 236
PpuMI rG'GwC_Cy
Cuts at: 0 141 526 540 1507 1575
Size: 141 385 14 967 68
Fragments arranged by size:
967 385 141 68 14
PshAI GACnn'nnGTC
Cuts at: 0 1493 1575
Size: 1493 82
PstI C_TGCA'G
Cuts at: 0 71 131 149 299 636 1367 1575
Size: 71 60 18 150 337 731 208
Fragments arranged by size:
731 337 208 150 71 60 18
PvuII CAG'CTG
Cuts at: 0 132 213 1575
Size: 132 81 1362
Fragments arranged by size:
1362 132 81
SacI G_AGCT'C
Cuts at: 0 959 1028 1575
Size: 959 69 547
Fragments arranged by size:
959 547 69
SanDI GG'GwC_CC
Cuts at: 0 540 1575
Size: 540 1035
SapI GCTCTTCn'nnn_
Cuts at: 0 340 385 1033 1575
Size: 340 45 648 542
Fragments arranged by size:
648 542 340 45
SbfI CC_TGCA'GG
Cuts at: 0 636 1575
Size: 636 939
SfcI C'TryA_G
Cuts at: 0 67 127 145 295 632 1363 1575
Size: 67 60 18 150 337 731 212
Fragments arranged by size:
731 337 212 150 67 60 18
SfoI GGC'GCC
Cuts at: 0 292 1575
Size: 292 1283
SmlI C'TyrA_G
Cuts at: 0 79 1575
Size: 79 1496
StyI C'CwwG_G
Cuts at: 0 98 769 1291 1338 1575
Size: 98 671 522 47 237
Fragments arranged by size:
671 522 237 98 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 719 1575
Size: 719 856
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 678 710 1575
Size: 678 32 865
Fragments arranged by size:
865 678 32
Enzymes that do cut:
AarI Acc65I AcuI AlwNI AvaI BanI BanII BbeI
BbvCI BcgI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstYI Bsu36I
BtgI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I HincII KasI KpnI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV PasI PflMI
PpuMI PshAI PstI PvuII SacI SanDI SapI SbfI
SfcI SfoI SmlI StyI TaqII TstI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BciVI BclI BfrBI
BglI BmtI BsaAI BsaBI BsiWI BsmI BspHI BsrGI
BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI MfeI MluI NdeI NheI NotI
NruI NsiI NspI PacI PciI PfoI PmeI PmlI
PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI