Restriction map for
pF1KB7795
(Linear) (Six-base) MAPSORT of: KIBB7795.seq Check: 8214 from: 1 to: 1383
KIBB7795.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
Acc65I G'GTAC_C
Cuts at: 0 259 1383
Size: 259 1124
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 274 347 410 1383
Size: 131 143 73 63 973
Fragments arranged by size:
973 143 131 73 63
AfeI AGC'GCT
Cuts at: 0 844 1383
Size: 844 539
AflIII A'CryG_T
Cuts at: 0 1219 1383
Size: 1219 164
AgeI A'CCGG_T
Cuts at: 0 546 1383
Size: 546 837
AlwNI CAG_nnn'CTG
Cuts at: 0 136 529 1383
Size: 136 393 854
Fragments arranged by size:
854 393 136
ApaI G_GGCC'C
Cuts at: 0 1054 1383
Size: 1054 329
AvaI C'yCGr_G
Cuts at: 0 156 745 826 1137 1383
Size: 156 589 81 311 246
Fragments arranged by size:
589 311 246 156 81
BanI G'GyrC_C
Cuts at: 0 259 740 917 1094 1383
Size: 259 481 177 177 289
Fragments arranged by size:
481 289 259 177 177
BanII G_rGCy'C
Cuts at: 0 69 269 559 574 816 956 1054 1383
Size: 69 200 290 15 242 140 98 329
Fragments arranged by size:
329 290 242 200 140 98 69 15
BbsI GAAGACnn'nnnn_
Cuts at: 0 137 1383
Size: 137 1246
BbvCI CC'TCA_GC
Cuts at: 0 456 643 1383
Size: 456 187 740
Fragments arranged by size:
740 456 187
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 780 814 1134 1168 1383
Size: 780 34 320 34 215
Fragments arranged by size:
780 320 215 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 223 683 1383
Size: 223 460 700
Fragments arranged by size:
700 460 223
BlpI GC'TnA_GC
Cuts at: 0 1081 1383
Size: 1081 302
Bme1580I G_kGCm'C
Cuts at: 0 910 1054 1383
Size: 910 144 329
Fragments arranged by size:
910 329 144
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 864 1383
Size: 864 519
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 487 1383
Size: 52 435 896
Fragments arranged by size:
896 435 52
Bpu10I CC'TnA_GC
Cuts at: 0 456 643 1383
Size: 456 187 740
Fragments arranged by size:
740 456 187
BsaAI yAC'GTr
Cuts at: 0 1220 1383
Size: 1220 163
BsaWI w'CCGG_w
Cuts at: 0 546 1383
Size: 546 837
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 370 395 400 425 1383
Size: 370 25 5 25 958
Fragments arranged by size:
958 370 25 25 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 228 1383
Size: 228 1155
BseYI C'CCAG_C
Cuts at: 0 406 621 706 1383
Size: 406 215 85 677
Fragments arranged by size:
677 406 215 85
BsiEI CG_ry'CG
Cuts at: 0 1242 1383
Size: 1242 141
BsiHKAI G_wGCw'C
Cuts at: 0 559 752 1160 1383
Size: 559 193 408 223
Fragments arranged by size:
559 408 223 193
BsmI GAATG_Cn'
Cuts at: 0 1126 1383
Size: 1126 257
BsmBI CGTCTCn'nnnn_
Cuts at: 0 431 1383
Size: 431 952
Bsp1286I G_dGCh'C
Cuts at: 0 69 269 559 574 752 816 910 956
Size: 69 200 290 15 178 64 94 46
Cuts at: 956 1054 1160 1383
Size: 98 106 223
Fragments arranged by size:
290 223 200 178 106 98 94 69
64 46 15
BsrBI CCG'CTC
Cuts at: 0 1311 1383
Size: 1311 72
BsrFI r'CCGG_y
Cuts at: 0 546 1237 1339 1383
Size: 546 691 102 44
Fragments arranged by size:
691 546 102 44
BsrGI T'GTAC_A
Cuts at: 0 176 1383
Size: 176 1207
BssHII G'CGCG_C
Cuts at: 0 1085 1335 1383
Size: 1085 250 48
Fragments arranged by size:
1085 250 48
BssSI C'ACGA_G
Cuts at: 0 976 1159 1383
Size: 976 183 224
Fragments arranged by size:
976 224 183
BstAPI GCAn_nnn'nTGC
Cuts at: 0 188 1121 1298 1383
Size: 188 933 177 85
Fragments arranged by size:
933 188 177 85
BstEII G'GTnAC_C
Cuts at: 0 894 1383
Size: 894 489
BstXI CCAn_nnnn'nTGG
Cuts at: 0 780 1383
Size: 780 603
DrdI GACnn_nn'nnGTC
Cuts at: 0 1108 1383
Size: 1108 275
EaeI y'GGCC_r
Cuts at: 0 114 1239 1383
Size: 114 1125 144
Fragments arranged by size:
1125 144 114
EagI C'GGCC_G
Cuts at: 0 1239 1383
Size: 1239 144
EarI CTCTTCn'nnn_
Cuts at: 0 246 1381 1383
Size: 246 1135 2
Fragments arranged by size:
1135 246 2
EcoICRI GAG'CTC
Cuts at: 0 557 1383
Size: 557 826
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 131 274 347 410 487 1383
Size: 52 79 143 73 63 77 896
Fragments arranged by size:
896 143 79 77 73 63 52
EcoNI CCTnn'n_nnAGG
Cuts at: 0 439 1383
Size: 439 944
EcoO109I rG'GnC_Cy
Cuts at: 0 6 1050 1051 1322 1383
Size: 6 1044 1 271 61
Fragments arranged by size:
1044 271 61 6 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1341 1383
Size: 1341 42
HaeII r_GCGC'y
Cuts at: 0 81 846 1081 1383
Size: 81 765 235 302
Fragments arranged by size:
765 302 235 81
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 370 395 402 427 1383
Size: 370 25 7 25 956
Fragments arranged by size:
956 370 25 25 7
KpnI G_GTAC'C
Cuts at: 0 263 1383
Size: 263 1120
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 662 906 1383
Size: 662 244 477
Fragments arranged by size:
662 477 244
MscI TGG'CCA
Cuts at: 0 116 1383
Size: 116 1267
MspA1I CmG'CkG
Cuts at: 0 248 1020 1244 1383
Size: 248 772 224 139
Fragments arranged by size:
772 248 224 139
NaeI GCC'GGC
Cuts at: 0 1341 1383
Size: 1341 42
NgoMIV G'CCGG_C
Cuts at: 0 1339 1383
Size: 1339 44
NspI r_CATG'y
Cuts at: 0 54 1292 1383
Size: 54 1238 91
Fragments arranged by size:
1238 91 54
PasI CC'CwG_GG
Cuts at: 0 780 1257 1383
Size: 780 477 126
Fragments arranged by size:
780 477 126
PflMI CCAn_nnn'nTGG
Cuts at: 0 112 529 859 1383
Size: 112 417 330 524
Fragments arranged by size:
524 417 330 112
PfoI T'CCnGG_A
Cuts at: 0 1058 1328 1383
Size: 1058 270 55
Fragments arranged by size:
1058 270 55
PmlI CAC'GTG
Cuts at: 0 1220 1383
Size: 1220 163
PpuMI rG'GwC_Cy
Cuts at: 0 6 1322 1383
Size: 6 1316 61
Fragments arranged by size:
1316 61 6
PspOMI G'GGCC_C
Cuts at: 0 1050 1383
Size: 1050 333
PstI C_TGCA'G
Cuts at: 0 216 635 1019 1117 1383
Size: 216 419 384 98 266
Fragments arranged by size:
419 384 266 216 98
PvuII CAG'CTG
Cuts at: 0 1020 1383
Size: 1020 363
SacI G_AGCT'C
Cuts at: 0 559 1383
Size: 559 824
SapI GCTCTTCn'nnn_
Cuts at: 0 1381 1383
Size: 1381 2
SfcI C'TryA_G
Cuts at: 0 212 631 1015 1113 1383
Size: 212 419 384 98 270
Fragments arranged by size:
419 384 270 212 98
SgrAI Cr'CCGG_yG
Cuts at: 0 546 1383
Size: 546 837
StuI AGG'CCT
Cuts at: 0 677 1383
Size: 677 706
StyI C'CwwG_G
Cuts at: 0 55 902 1316 1383
Size: 55 847 414 67
Fragments arranged by size:
847 414 67 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 188 679 935 1383
Size: 188 491 256 448
Fragments arranged by size:
491 448 256 188
TatI w'GTAC_w
Cuts at: 0 176 1383
Size: 176 1207
Tth111I GACn'n_nGTC
Cuts at: 0 132 1383
Size: 132 1251
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 252 468 1383
Size: 252 216 915
Fragments arranged by size:
915 252 216
XmnI GAAnn'nnTTC
Cuts at: 0 1186 1375 1383
Size: 1186 189 8
Fragments arranged by size:
1186 189 8
Enzymes that do cut:
Acc65I AcuI AfeI AflIII AgeI AlwNI ApaI AvaI
BanI BanII BbsI BbvCI BcgI BglI BlpI Bme1580I
BplI BpmI Bpu10I BsaAI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstEII BstXI DrdI EaeI EagI
EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
KpnI MmeI MscI MspA1I NaeI NgoMIV NspI PasI
PflMI PfoI PmlI PpuMI PspOMI PstI PvuII SacI
SapI SfcI SgrAI StuI StyI TaqII TatI Tth111I
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AhdI AleI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BciVI BclI BfrBI BglII BmgBI BmrI
BmtI BpuEI BsaI BsaBI BsaHI BsgI BsiWI BspEI
BspHI BspMI BsrDI BstBI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII EciI EcoRI
EcoRV FseI FspI FspAI HincII HindIII HpaI KasI
MfeI MluI MslI NarI NcoI NdeI NheI NotI
NruI NsiI PacI PciI PmeI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TstI XbaI XhoI
XmaI ZraI