Restriction map for
pF1KB7787
(Linear) (Six-base) MAPSORT of: KIBB7787.seq Check: 8909 from: 1 to: 1374
KIBB7787.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 0 1301 1374
Size: 1301 73
Acc65I G'GTAC_C
Cuts at: 0 161 1374
Size: 161 1213
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 344 379 492 1080 1374
Size: 344 35 113 588 294
Fragments arranged by size:
588 344 294 113 35
AflIII A'CryG_T
Cuts at: 0 394 1374
Size: 394 980
AhdI GACnn_n'nnGTC
Cuts at: 0 297 1374
Size: 297 1077
ApaI G_GGCC'C
Cuts at: 0 111 1374
Size: 111 1263
ApaLI G'TGCA_C
Cuts at: 0 61 1374
Size: 61 1313
ApoI r'AATT_y
Cuts at: 0 135 317 1046 1130 1351 1374
Size: 135 182 729 84 221 23
Fragments arranged by size:
729 221 182 135 84 23
AseI AT'TA_AT
Cuts at: 0 307 1374
Size: 307 1067
BanI G'GyrC_C
Cuts at: 0 161 617 1037 1374
Size: 161 456 420 337
Fragments arranged by size:
456 420 337 161
BanII G_rGCy'C
Cuts at: 0 111 1374
Size: 111 1263
BbsI GAAGACnn'nnnn_
Cuts at: 0 1139 1374
Size: 1139 235
BclI T'GATC_A
Cuts at: 0 695 1374
Size: 695 679
BglI GCCn_nnn'nGGC
Cuts at: 0 106 1374
Size: 106 1268
Bme1580I G_kGCm'C
Cuts at: 0 65 111 1374
Size: 65 46 1263
Fragments arranged by size:
1263 65 46
BmrI ACTGGGnnnn_n'
Cuts at: 0 160 560 812 1374
Size: 160 400 252 562
Fragments arranged by size:
562 400 252 160
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 386 1374
Size: 386 988
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 656 908 1244 1328 1374
Size: 656 252 336 84 46
Fragments arranged by size:
656 336 252 84 46
Bpu10I CC'TnA_GC
Cuts at: 0 1103 1374
Size: 1103 271
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1312 1374
Size: 1312 62
BsaI GGTCTCn'nnnn_
Cuts at: 0 274 636 1374
Size: 274 362 738
Fragments arranged by size:
738 362 274
BsaAI yAC'GTr
Cuts at: 0 397 1374
Size: 397 977
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 505 673 1072 1374
Size: 505 168 399 302
Fragments arranged by size:
505 399 302 168
BseYI C'CCAG_C
Cuts at: 0 243 1277 1374
Size: 243 1034 97
Fragments arranged by size:
1034 243 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 1374
Size: 164 1210
BsiHKAI G_wGCw'C
Cuts at: 0 65 1104 1374
Size: 65 1039 270
Fragments arranged by size:
1039 270 65
Bsp1286I G_dGCh'C
Cuts at: 0 65 111 1104 1374
Size: 65 46 993 270
Fragments arranged by size:
993 270 65 46
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 186 1374
Size: 186 1188
BsrGI T'GTAC_A
Cuts at: 0 739 1374
Size: 739 635
BstEII G'GTnAC_C
Cuts at: 0 39 1285 1374
Size: 39 1246 89
Fragments arranged by size:
1246 89 39
EarI CTCTTCn'nnn_
Cuts at: 0 355 1374
Size: 355 1019
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 344 379 492 656 908 1080 1244 1328
Size: 344 35 113 164 252 172 164 84
Cuts at: 1328 1374
Size: 46
Fragments arranged by size:
344 252 172 164 164 113 84 46
35
EcoNI CCTnn'n_nnAGG
Cuts at: 0 468 1374
Size: 468 906
EcoO109I rG'GnC_Cy
Cuts at: 0 107 108 219 1374
Size: 107 1 111 1155
Fragments arranged by size:
1155 111 107 1
EcoRI G'AATT_C
Cuts at: 0 1046 1130 1374
Size: 1046 84 244
Fragments arranged by size:
1046 244 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 494 1374
Size: 494 880
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 283 315 633 665 687 719 927 959
Size: 283 32 318 32 22 32 208 32
Cuts at: 959 1374
Size: 415
Fragments arranged by size:
415 318 283 208 32 32 32 32
22
HindIII A'AGCT_T
Cuts at: 0 839 924 1374
Size: 839 85 450
Fragments arranged by size:
839 450 85
KpnI G_GTAC'C
Cuts at: 0 165 1374
Size: 165 1209
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 1374
Size: 110 1264
MspA1I CmG'CkG
Cuts at: 0 357 1374
Size: 357 1017
NdeI CA'TA_TG
Cuts at: 0 484 1374
Size: 484 890
NspI r_CATG'y
Cuts at: 0 1300 1374
Size: 1300 74
PflMI CCAn_nnn'nTGG
Cuts at: 0 36 1374
Size: 36 1338
PshAI GACnn'nnGTC
Cuts at: 0 1331 1374
Size: 1331 43
PspOMI G'GGCC_C
Cuts at: 0 107 1374
Size: 107 1267
PvuII CAG'CTG
Cuts at: 0 357 1374
Size: 357 1017
SmlI C'TyrA_G
Cuts at: 0 1291 1374
Size: 1291 83
StuI AGG'CCT
Cuts at: 0 1261 1374
Size: 1261 113
StyI C'CwwG_G
Cuts at: 0 441 1374
Size: 441 933
TatI w'GTAC_w
Cuts at: 0 739 1374
Size: 739 635
XmnI GAAnn'nnTTC
Cuts at: 0 478 505 757 936 1374
Size: 478 27 252 179 438
Fragments arranged by size:
478 438 252 179 27
Enzymes that do cut:
AccI Acc65I AcuI AflIII AhdI ApaI ApaLI ApoI
AseI BanI BanII BbsI BclI BglI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspMI BsrGI BstEII EarI Eco57MI
EcoNI EcoO109I EcoRI FalI Hin4I HindIII KpnI MmeI
MspA1I NdeI NspI PflMI PshAI PspOMI PvuII SmlI
StuI StyI TatI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AleI AlfI
AloI AlwNI AscI AsiSI AvaI AvrII BaeI BamHI
BbeI BbvCI BcgI BciVI BfrBI BglII BlpI BmgBI
BmtI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EciI EcoICRI EcoRV FseI FspI FspAI HaeII HincII
HpaI KasI MfeI MluI MscI MslI NaeI NarI
NcoI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PsiI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TaqII TstI Tth111I XbaI XcmI XhoI XmaI ZraI