Restriction map for
pF1KB7784
(Linear) (Six-base) MAPSORT of: KIBB7784.seq Check: 7392 from: 1 to: 1530
KIBB7784.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 1104 1530
Size: 193 911 426
Fragments arranged by size:
911 426 193
AfeI AGC'GCT
Cuts at: 0 141 1530
Size: 141 1389
AflIII A'CryG_T
Cuts at: 0 1432 1530
Size: 1432 98
AleI CACnn'nnGTG
Cuts at: 0 1415 1530
Size: 1415 115
AlwNI CAG_nnn'CTG
Cuts at: 0 231 291 360 1530
Size: 231 60 69 1170
Fragments arranged by size:
1170 231 69 60
ApoI r'AATT_y
Cuts at: 0 373 1154 1322 1530
Size: 373 781 168 208
Fragments arranged by size:
781 373 208 168
BanII G_rGCy'C
Cuts at: 0 497 1530
Size: 497 1033
BbsI GAAGACnn'nnnn_
Cuts at: 0 775 1530
Size: 775 755
BbvCI CC'TCA_GC
Cuts at: 0 232 1530
Size: 232 1298
BclI T'GATC_A
Cuts at: 0 612 852 1530
Size: 612 240 678
Fragments arranged by size:
678 612 240
BglII A'GATC_T
Cuts at: 0 260 1218 1530
Size: 260 958 312
Fragments arranged by size:
958 312 260
BmrI ACTGGGnnnn_n'
Cuts at: 0 330 1530
Size: 330 1200
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 264 1530
Size: 264 1266
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 177 1184 1530
Size: 72 105 1007 346
Fragments arranged by size:
1007 346 105 72
Bpu10I CC'TnA_GC
Cuts at: 0 232 292 1530
Size: 232 60 1238
Fragments arranged by size:
1238 232 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 18 211 784 1530
Size: 18 193 573 746
Fragments arranged by size:
746 573 193 18
BsaI GGTCTCn'nnnn_
Cuts at: 0 304 1530
Size: 304 1226
BsaWI w'CCGG_w
Cuts at: 0 181 1530
Size: 181 1349
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 150 180 1530
Size: 150 30 1350
Fragments arranged by size:
1350 150 30
BseYI C'CCAG_C
Cuts at: 0 426 558 584 1530
Size: 426 132 26 946
Fragments arranged by size:
946 426 132 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 1530
Size: 346 1184
BsiEI CG_ry'CG
Cuts at: 0 1468 1530
Size: 1468 62
BsiHKAI G_wGCw'C
Cuts at: 0 446 1530
Size: 446 1084
BsmI GAATG_Cn'
Cuts at: 0 296 1530
Size: 296 1234
Bsp1286I G_dGCh'C
Cuts at: 0 446 497 1530
Size: 446 51 1033
Fragments arranged by size:
1033 446 51
BspHI T'CATG_A
Cuts at: 0 171 1235 1530
Size: 171 1064 295
Fragments arranged by size:
1064 295 171
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1029 1530
Size: 1029 501
BsrGI T'GTAC_A
Cuts at: 0 975 1530
Size: 975 555
BstYI r'GATC_y
Cuts at: 0 242 260 1056 1218 1530
Size: 242 18 796 162 312
Fragments arranged by size:
796 312 242 162 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 19 1530
Size: 19 1511
BtsI GCAGTG_nn'
Cuts at: 0 446 467 1530
Size: 446 21 1063
Fragments arranged by size:
1063 446 21
DraI TTT'AAA
Cuts at: 0 1347 1530
Size: 1347 183
DraIII CAC_nnn'GTG
Cuts at: 0 973 1530
Size: 973 557
DrdI GACnn_nn'nnGTC
Cuts at: 0 69 1530
Size: 69 1461
EarI CTCTTCn'nnn_
Cuts at: 0 734 1530
Size: 734 796
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 177 193 1104 1184 1530
Size: 72 105 16 911 80 346
Fragments arranged by size:
911 346 105 80 72 16
EcoO109I rG'GnC_Cy
Cuts at: 0 1072 1530
Size: 1072 458
EcoRI G'AATT_C
Cuts at: 0 373 1154 1322 1530
Size: 373 781 168 208
Fragments arranged by size:
781 373 208 168
EcoRV GAT'ATC
Cuts at: 0 798 1530
Size: 798 732
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1470 1530
Size: 1470 60
HaeII r_GCGC'y
Cuts at: 0 143 1530
Size: 143 1387
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 16 48 1203 1235 1530
Size: 16 32 1155 32 295
Fragments arranged by size:
1155 295 32 32 16
HincII GTy'rAC
Cuts at: 0 704 1186 1530
Size: 704 482 344
Fragments arranged by size:
704 482 344
HindIII A'AGCT_T
Cuts at: 0 1116 1284 1530
Size: 1116 168 246
Fragments arranged by size:
1116 246 168
MslI CAynn'nnrTG
Cuts at: 0 894 1263 1415 1530
Size: 894 369 152 115
Fragments arranged by size:
894 369 152 115
MspA1I CmG'CkG
Cuts at: 0 558 1530
Size: 558 972
NspI r_CATG'y
Cuts at: 0 1436 1514 1530
Size: 1436 78 16
Fragments arranged by size:
1436 78 16
PciI A'CATG_T
Cuts at: 0 1432 1530
Size: 1432 98
PflMI CCAn_nnn'nTGG
Cuts at: 0 426 504 1530
Size: 426 78 1026
Fragments arranged by size:
1026 426 78
PvuI CG_AT'CG
Cuts at: 0 1468 1530
Size: 1468 62
PvuII CAG'CTG
Cuts at: 0 558 1530
Size: 558 972
RsrII CG'GwC_CG
Cuts at: 0 184 1530
Size: 184 1346
SmlI C'TyrA_G
Cuts at: 0 33 190 763 1530
Size: 33 157 573 767
Fragments arranged by size:
767 573 157 33
SpeI A'CTAG_T
Cuts at: 0 64 1530
Size: 64 1466
SspI AAT'ATT
Cuts at: 0 526 1530
Size: 526 1004
StyI C'CwwG_G
Cuts at: 0 305 1000 1530
Size: 305 695 530
Fragments arranged by size:
695 530 305
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 312 496 1530
Size: 312 184 1034
Fragments arranged by size:
1034 312 184
TatI w'GTAC_w
Cuts at: 0 975 1530
Size: 975 555
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 446 478 1530
Size: 446 32 1052
Fragments arranged by size:
1052 446 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 476 1530
Size: 476 1054
XmnI GAAnn'nnTTC
Cuts at: 0 1117 1530
Size: 1117 413
Enzymes that do cut:
AcuI AfeI AflIII AleI AlwNI ApoI BanII BbsI
BbvCI BclI BglII BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrGI BstYI BtgZI BtsI DraI
DraIII DrdI EarI Eco57MI EcoO109I EcoRI EcoRV FalI
HaeII Hin4I HincII HindIII MslI MspA1I NspI PciI
PflMI PvuI PvuII RsrII SmlI SpeI SspI StyI
TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BcgI BciVI BfrBI
BglI BlpI Bme1580I BmgBI BmtI BsaAI BsaBI BsaHI
BseRI BsiWI BsmBI BspEI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI EaeI EagI EciI EcoICRI EcoNI FseI
FspI FspAI HpaI KasI KpnI MfeI MluI MmeI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI
StuI SwaI Tth111I XbaI XhoI XmaI ZraI