Restriction map for
pF1KB7779
(Linear) (Six-base) MAPSORT of: KIBB7779.seq Check: 353 from: 1 to: 1302
KIBB7779.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 225 410 708 1302
Size: 225 185 298 594
Fragments arranged by size:
594 298 225 185
AfeI AGC'GCT
Cuts at: 0 236 1302
Size: 236 1066
AflIII A'CryG_T
Cuts at: 0 537 571 1046 1302
Size: 537 34 475 256
Fragments arranged by size:
537 475 256 34
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 94 1302
Size: 94 1208
AlwNI CAG_nnn'CTG
Cuts at: 0 30 367 397 415 964 1068 1302
Size: 30 337 30 18 549 104 234
Fragments arranged by size:
549 337 234 104 30 30 18
ApaLI G'TGCA_C
Cuts at: 0 1282 1302
Size: 1282 20
ApoI r'AATT_y
Cuts at: 0 526 625 1002 1035 1182 1302
Size: 526 99 377 33 147 120
Fragments arranged by size:
526 377 147 120 99 33
BanII G_rGCy'C
Cuts at: 0 294 1302
Size: 294 1008
BbvCI CC'TCA_GC
Cuts at: 0 772 1302
Size: 772 530
BciVI GTATCCnnnnn_n'
Cuts at: 0 855 1119 1284 1302
Size: 855 264 165 18
Fragments arranged by size:
855 264 165 18
Bme1580I G_kGCm'C
Cuts at: 0 1286 1302
Size: 1286 16
BmgBI CAC'GTC
Cuts at: 0 574 1302
Size: 574 728
BmrI ACTGGGnnnn_n'
Cuts at: 0 782 1302
Size: 782 520
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 437 520 1302
Size: 437 83 782
Fragments arranged by size:
782 437 83
Bpu10I CC'TnA_GC
Cuts at: 0 772 1302
Size: 772 530
BsaI GGTCTCn'nnnn_
Cuts at: 0 280 1302
Size: 280 1022
BsaWI w'CCGG_w
Cuts at: 0 1006 1302
Size: 1006 296
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1094 1124 1302
Size: 1094 30 178
Fragments arranged by size:
1094 178 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 302 313 820 1302
Size: 302 11 507 482
Fragments arranged by size:
507 482 302 11
BseYI C'CCAG_C
Cuts at: 0 317 1302
Size: 317 985
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 929 988 1302
Size: 82 847 59 314
Fragments arranged by size:
847 314 82 59
BsiEI CG_ry'CG
Cuts at: 0 935 1302
Size: 935 367
BsiHKAI G_wGCw'C
Cuts at: 0 1286 1302
Size: 1286 16
Bsp1286I G_dGCh'C
Cuts at: 0 294 1286 1302
Size: 294 992 16
Fragments arranged by size:
992 294 16
BspHI T'CATG_A
Cuts at: 0 158 1052 1302
Size: 158 894 250
Fragments arranged by size:
894 250 158
BsrGI T'GTAC_A
Cuts at: 0 1190 1302
Size: 1190 112
BstAPI GCAn_nnn'nTGC
Cuts at: 0 397 1302
Size: 397 905
BtgI C'CryG_G
Cuts at: 0 404 1302
Size: 404 898
DraI TTT'AAA
Cuts at: 0 1208 1302
Size: 1208 94
DraIII CAC_nnn'GTG
Cuts at: 0 60 1302
Size: 60 1242
EaeI y'GGCC_r
Cuts at: 0 32 51 96 812 932 1302
Size: 32 19 45 716 120 370
Fragments arranged by size:
716 370 120 45 32 19
EagI C'GGCC_G
Cuts at: 0 932 1302
Size: 932 370
EarI CTCTTCn'nnn_
Cuts at: 0 244 1302
Size: 244 1058
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 124 1302
Size: 124 1178
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 225 410 437 520 708 1302
Size: 225 185 27 83 188 594
Fragments arranged by size:
594 225 188 185 83 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 371 1265 1302
Size: 371 894 37
Fragments arranged by size:
894 371 37
EcoO109I rG'GnC_Cy
Cuts at: 0 303 364 1091 1302
Size: 303 61 727 211
Fragments arranged by size:
727 303 211 61
HaeII r_GCGC'y
Cuts at: 0 238 1302
Size: 238 1064
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 27 1302
Size: 27 1275
MfeI C'AATT_G
Cuts at: 0 849 1302
Size: 849 453
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1080 1302
Size: 1080 222
MscI TGG'CCA
Cuts at: 0 53 814 1302
Size: 53 761 488
Fragments arranged by size:
761 488 53
MslI CAynn'nnrTG
Cuts at: 0 640 832 1051 1057 1302
Size: 640 192 219 6 245
Fragments arranged by size:
640 245 219 192 6
MspA1I CmG'CkG
Cuts at: 0 397 406 943 964 1302
Size: 397 9 537 21 338
Fragments arranged by size:
537 397 338 21 9
NspI r_CATG'y
Cuts at: 0 208 521 541 1050 1062 1302
Size: 208 313 20 509 12 240
Fragments arranged by size:
509 313 240 208 20 12
PciI A'CATG_T
Cuts at: 0 537 1046 1302
Size: 537 509 256
Fragments arranged by size:
537 509 256
PflMI CCAn_nnn'nTGG
Cuts at: 0 180 1039 1302
Size: 180 859 263
Fragments arranged by size:
859 263 180
PfoI T'CCnGG_A
Cuts at: 0 13 1302
Size: 13 1289
PpuMI rG'GwC_Cy
Cuts at: 0 303 364 1091 1302
Size: 303 61 727 211
Fragments arranged by size:
727 303 211 61
PstI C_TGCA'G
Cuts at: 0 26 402 1302
Size: 26 376 900
Fragments arranged by size:
900 376 26
PvuII CAG'CTG
Cuts at: 0 397 943 964 1302
Size: 397 546 21 338
Fragments arranged by size:
546 397 338 21
SacII CC_GC'GG
Cuts at: 0 407 1302
Size: 407 895
SanDI GG'GwC_CC
Cuts at: 0 1091 1302
Size: 1091 211
SapI GCTCTTCn'nnn_
Cuts at: 0 244 1302
Size: 244 1058
SfcI C'TryA_G
Cuts at: 0 22 398 1161 1262 1302
Size: 22 376 763 101 40
Fragments arranged by size:
763 376 101 40 22
SspI AAT'ATT
Cuts at: 0 757 1302
Size: 757 545
StyI C'CwwG_G
Cuts at: 0 1086 1173 1302
Size: 1086 87 129
Fragments arranged by size:
1086 129 87
SwaI ATTT'AAAT
Cuts at: 0 1208 1302
Size: 1208 94
TatI w'GTAC_w
Cuts at: 0 1190 1302
Size: 1190 112
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1094 1126 1302
Size: 1094 32 176
Fragments arranged by size:
1094 176 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 807 1302
Size: 807 495
Enzymes that do cut:
AcuI AfeI AflIII AlfI AlwNI ApaLI ApoI BanII
BbvCI BciVI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspHI BsrGI BstAPI BtgI DraI DraIII EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I MfeI
MmeI MscI MslI MspA1I NspI PciI PflMI PfoI
PpuMI PstI PvuII SacII SanDI SapI SfcI SspI
StyI SwaI TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AleI AloI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbsI BcgI BclI BfrBI
BglI BglII BlpI BmtI BplI BpuEI BsaAI BsaBI
BsaHI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DrdI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SalI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI
TaqII Tth111I XbaI XhoI XmaI XmnI ZraI