(Linear) (Six-base) MAPSORT of: KIBB7766.seq  Check: 8441  from: 1  to: 1509



KIBB7766.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:27  ..

AccI GT'mk_AC

Cuts at:      0     318    1509 
   Size:        318    1191
 
AgeI A'CCGG_T

Cuts at:      0    1385    1509 
   Size:       1385     124
 
ApoI r'AATT_y

Cuts at:      0      34      78     189     327     466     474     578     743 
   Size:         34      44     111     138     139       8     104     165

Cuts at:    743     787     827     911     995    1180    1247    1331    1509 
   Size:         44      40      84      84     185      67      84     178

  Fragments arranged by size: 

                 185     178     165     139     138     111     104      84
                  84      84      67      44      44      40      34       8
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     114     202    1509 
   Size:        114      88    1307

  Fragments arranged by size: 

                1307     114      88
 
BclI T'GATC_A

Cuts at:      0     155    1509 
   Size:        155    1354
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     217    1509 
   Size:        217    1292
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     187     608     773     857    1109    1277    1361 
   Size:         64     123     421     165      84     252     168      84

Cuts at:   1361    1509 
   Size:        148

  Fragments arranged by size: 

                 421     252     168     165     148     123      84      84
                  64
 
BsaWI w'CCGG_w

Cuts at:      0    1385    1509 
   Size:       1385     124
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      61    1509 
   Size:         61    1448
 
BsmI GAATG_Cn'

Cuts at:      0     548    1509 
   Size:        548     961
 
BsrDI GCAATG_nn'

Cuts at:      0      61    1509 
   Size:         61    1448
 
BsrFI r'CCGG_y

Cuts at:      0    1385    1509 
   Size:       1385     124
 
BstBI TT'CG_AA

Cuts at:      0     505    1509 
   Size:        505    1004
 
EaeI y'GGCC_r

Cuts at:      0      26     256    1509 
   Size:         26     230    1253

  Fragments arranged by size: 

                1253     230      26
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     187     608     773     857    1109    1277    1361 
   Size:         64     123     421     165      84     252     168      84

Cuts at:   1361    1509 
   Size:        148

  Fragments arranged by size: 

                 421     252     168     165     148     123      84      84
                  64
 
EcoRI G'AATT_C

Cuts at:      0      34     743     827     995    1247    1331    1509 
   Size:         34     709      84     168     252      84     178

  Fragments arranged by size: 

                 709     252     178     168      84      84      34
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     518    1509 
   Size:        518     991
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     147     179    1509 
   Size:        147      32    1330

  Fragments arranged by size: 

                1330     147      32
 
HincII GTy'rAC

Cuts at:      0      11    1509 
   Size:         11    1498
 
HindIII A'AGCT_T

Cuts at:      0     956    1040    1124    1376    1509 
   Size:        956      84      84     252     133

  Fragments arranged by size: 

                 956     252     133      84      84
 
MscI TGG'CCA

Cuts at:      0      28     258    1509 
   Size:         28     230    1251

  Fragments arranged by size: 

                1251     230      28
 
MslI CAynn'nnrTG

Cuts at:      0     936    1509 
   Size:        936     573
 
PsiI TTA'TAA

Cuts at:      0     389    1509 
   Size:        389    1120
 
SexAI A'CCwGG_T

Cuts at:      0     116    1509 
   Size:        116    1393
 
SfcI C'TryA_G

Cuts at:      0     108    1172    1509 
   Size:        108    1064     337

  Fragments arranged by size: 

                1064     337     108
 
SspI AAT'ATT

Cuts at:      0     431    1509 
   Size:        431    1078
 
StyI C'CwwG_G

Cuts at:      0     184    1509 
   Size:        184    1325
 
TatI w'GTAC_w

Cuts at:      0     646    1509 
   Size:        646     863
 
XmnI GAAnn'nnTTC

Cuts at:      0     514    1509 
   Size:        514     995
 

 Enzymes that do cut:

     AccI     AgeI     ApoI     BbsI     BclI     BmrI     BpmI    BsaWI
    BseRI     BsmI    BsrDI    BsrFI    BstBI     EaeI  Eco57MI    EcoRI
     FalI    Hin4I   HincII  HindIII     MscI     MslI     PsiI    SexAI
     SfcI     SspI     StyI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrGI   BssHII
    BssSI   BstAPI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FseI     FspI
    FspAI    HaeII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI    TaqII
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI