Restriction map for
pF1KB7763
(Linear) (Six-base) MAPSORT of: KIBB7763.seq Check: 4025 from: 1 to: 1344
KIBB7763.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 169 1344
Size: 169 1175
AccI GT'mk_AC
Cuts at: 0 209 1344
Size: 209 1135
AclI AA'CG_TT
Cuts at: 0 70 1344
Size: 70 1274
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 1344
Size: 328 1016
AflII C'TTAA_G
Cuts at: 0 1159 1344
Size: 1159 185
AleI CACnn'nnGTG
Cuts at: 0 667 823 1344
Size: 667 156 521
Fragments arranged by size:
667 521 156
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 248 1344
Size: 248 1096
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 905 937 1344
Size: 905 32 407
Fragments arranged by size:
905 407 32
ApoI r'AATT_y
Cuts at: 0 403 654 762 1344
Size: 403 251 108 582
Fragments arranged by size:
582 403 251 108
BanI G'GyrC_C
Cuts at: 0 85 1344
Size: 85 1259
BanII G_rGCy'C
Cuts at: 0 1104 1344
Size: 1104 240
BbeI G_GCGC'C
Cuts at: 0 89 1344
Size: 89 1255
BbsI GAAGACnn'nnnn_
Cuts at: 0 1027 1344
Size: 1027 317
BbvCI CC'TCA_GC
Cuts at: 0 695 1226 1344
Size: 695 531 118
Fragments arranged by size:
695 531 118
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 229 263 1344
Size: 229 34 1081
Fragments arranged by size:
1081 229 34
BmgBI CAC'GTC
Cuts at: 0 560 1344
Size: 560 784
BmrI ACTGGGnnnn_n'
Cuts at: 0 1295 1344
Size: 1295 49
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 454 893 1066 1186 1344
Size: 451 3 439 173 120 158
Fragments arranged by size:
451 439 173 158 120 3
Bpu10I CC'TnA_GC
Cuts at: 0 695 1226 1344
Size: 695 531 118
Fragments arranged by size:
695 531 118
BsaHI Gr'CG_yC
Cuts at: 0 86 1344
Size: 86 1258
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 371 401 1106 1136 1344
Size: 371 30 705 30 208
Fragments arranged by size:
705 371 208 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 967 1344
Size: 967 377
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 1344
Size: 302 1042
BsmI GAATG_Cn'
Cuts at: 0 297 1344
Size: 297 1047
Bsp1286I G_dGCh'C
Cuts at: 0 1104 1344
Size: 1104 240
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 169 942 1344
Size: 169 773 402
Fragments arranged by size:
773 402 169
BsrDI GCAATG_nn'
Cuts at: 0 482 1059 1344
Size: 482 577 285
Fragments arranged by size:
577 482 285
BstAPI GCAn_nnn'nTGC
Cuts at: 0 691 1344
Size: 691 653
BstEII G'GTnAC_C
Cuts at: 0 622 1344
Size: 622 722
Bsu36I CC'TnA_GG
Cuts at: 0 618 1344
Size: 618 726
BtgI C'CryG_G
Cuts at: 0 1208 1325 1344
Size: 1208 117 19
Fragments arranged by size:
1208 117 19
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1013 1048 1344
Size: 1013 35 296
Fragments arranged by size:
1013 296 35
DraIII CAC_nnn'GTG
Cuts at: 0 258 1344
Size: 258 1086
EaeI y'GGCC_r
Cuts at: 0 882 1344
Size: 882 462
EarI CTCTTCn'nnn_
Cuts at: 0 974 1092 1211 1259 1344
Size: 974 118 119 48 85
Fragments arranged by size:
974 119 118 85 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 451 454 893 1066 1186 1344
Size: 328 123 3 439 173 120 158
Fragments arranged by size:
439 328 173 158 123 120 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 872 1344
Size: 872 472
EcoO109I rG'GnC_Cy
Cuts at: 0 215 1344
Size: 215 1129
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 187 1344
Size: 187 1157
FspI TGC'GCA
Cuts at: 0 251 1344
Size: 251 1093
FspAI rTGC'GCAy
Cuts at: 0 251 1344
Size: 251 1093
HaeII r_GCGC'y
Cuts at: 0 89 342 1344
Size: 89 253 1002
Fragments arranged by size:
1002 253 89
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 371 403 1106 1138 1344
Size: 371 32 703 32 206
Fragments arranged by size:
703 371 206 32 32
HincII GTy'rAC
Cuts at: 0 576 771 1344
Size: 576 195 573
Fragments arranged by size:
576 573 195
HpaI GTT'AAC
Cuts at: 0 576 1344
Size: 576 768
KasI G'GCGC_C
Cuts at: 0 85 1344
Size: 85 1259
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 740 1241 1344
Size: 740 501 103
Fragments arranged by size:
740 501 103
MscI TGG'CCA
Cuts at: 0 884 1344
Size: 884 460
MslI CAynn'nnrTG
Cuts at: 0 350 667 823 1344
Size: 350 317 156 521
Fragments arranged by size:
521 350 317 156
NarI GG'CG_CC
Cuts at: 0 86 1344
Size: 86 1258
NcoI C'CATG_G
Cuts at: 0 1208 1325 1344
Size: 1208 117 19
Fragments arranged by size:
1208 117 19
NspI r_CATG'y
Cuts at: 0 250 306 1344
Size: 250 56 1038
Fragments arranged by size:
1038 250 56
PflMI CCAn_nnn'nTGG
Cuts at: 0 104 1344
Size: 104 1240
PfoI T'CCnGG_A
Cuts at: 0 467 1082 1344
Size: 467 615 262
Fragments arranged by size:
615 467 262
PpuMI rG'GwC_Cy
Cuts at: 0 215 1344
Size: 215 1129
PstI C_TGCA'G
Cuts at: 0 237 1344
Size: 237 1107
SapI GCTCTTCn'nnn_
Cuts at: 0 1092 1344
Size: 1092 252
ScaI AGT'ACT
Cuts at: 0 167 1344
Size: 167 1177
SfcI C'TryA_G
Cuts at: 0 233 1069 1238 1344
Size: 233 836 169 106
Fragments arranged by size:
836 233 169 106
SfoI GGC'GCC
Cuts at: 0 87 1344
Size: 87 1257
SmlI C'TyrA_G
Cuts at: 0 1159 1344
Size: 1159 185
StyI C'CwwG_G
Cuts at: 0 296 1006 1208 1325 1344
Size: 296 710 202 117 19
Fragments arranged by size:
710 296 202 117 19
TatI w'GTAC_w
Cuts at: 0 165 642 1344
Size: 165 477 702
Fragments arranged by size:
702 477 165
Tth111I GACn'n_nGTC
Cuts at: 0 1287 1344
Size: 1287 57
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 669 1344
Size: 669 675
Enzymes that do cut:
AarI AccI AclI AcuI AflII AleI AlfI AloI
ApoI BanI BanII BbeI BbsI BbvCI BcgI BmgBI
BmrI BpmI Bpu10I BsaHI BsaXI BseRI BsgI BsmI
Bsp1286I BspMI BsrDI BstAPI BstEII Bsu36I BtgI CspCI
DraIII EaeI EarI Eco57MI EcoNI EcoO109I FalI FspI
FspAI HaeII Hin4I HincII HpaI KasI MmeI MscI
MslI NarI NcoI NspI PflMI PfoI PpuMI PstI
SapI ScaI SfcI SfoI SmlI StyI TatI Tth111I
XcmI
Enzymes that do not cut:
AatII Acc65I AfeI AflIII AgeI AhdI AlwNI ApaI
ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaWI BseYI BsiEI
BsiHKAI BsiWI BsmBI BspEI BspHI BsrBI BsrFI BsrGI
BssHII BssSI BstBI BstXI BstYI BstZ17I BtgZI BtsI
ClaI DraI DrdI EagI EciI EcoICRI EcoRI EcoRV
FseI HindIII KpnI MfeI MluI MspA1I NaeI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SbfI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TstI XbaI XhoI
XmaI XmnI ZraI