Restriction map for
pF1KB7755
(Linear) (Six-base) MAPSORT of: KIBB7755.seq Check: 3684 from: 1 to: 1338
KIBB7755.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 210 386 1338
Size: 210 176 952
Fragments arranged by size:
952 210 176
AatII G_ACGT'C
Cuts at: 0 845 1338
Size: 845 493
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 632 751 765 1338
Size: 632 119 14 573
Fragments arranged by size:
632 573 119 14
AfeI AGC'GCT
Cuts at: 0 187 1338
Size: 187 1151
AhdI GACnn_n'nnGTC
Cuts at: 0 213 1338
Size: 213 1125
AleI CACnn'nnGTG
Cuts at: 0 956 1338
Size: 956 382
AlwNI CAG_nnn'CTG
Cuts at: 0 864 1301 1338
Size: 864 437 37
Fragments arranged by size:
864 437 37
ApaI G_GGCC'C
Cuts at: 0 951 1338
Size: 951 387
AscI GG'CGCG_CC
Cuts at: 0 346 1338
Size: 346 992
AvaI C'yCGr_G
Cuts at: 0 155 561 889 1055 1338
Size: 155 406 328 166 283
Fragments arranged by size:
406 328 283 166 155
BanI G'GyrC_C
Cuts at: 0 49 151 249 595 632 715 764 823
Size: 49 102 98 346 37 83 49 59
Cuts at: 823 913 1177 1338
Size: 90 264 161
Fragments arranged by size:
346 264 161 102 98 90 83 59
49 49 37
BanII G_rGCy'C
Cuts at: 0 395 806 951 1033 1338
Size: 395 411 145 82 305
Fragments arranged by size:
411 395 305 145 82
BbeI G_GCGC'C
Cuts at: 0 155 719 768 917 1338
Size: 155 564 49 149 421
Fragments arranged by size:
564 421 155 149 49
BglI GCCn_nnn'nGGC
Cuts at: 0 47 967 1043 1105 1236 1338
Size: 47 920 76 62 131 102
Fragments arranged by size:
920 131 102 76 62 47
BlpI GC'TnA_GC
Cuts at: 0 394 427 805 1338
Size: 394 33 378 533
Fragments arranged by size:
533 394 378 33
Bme1580I G_kGCm'C
Cuts at: 0 254 635 826 951 963 1338
Size: 254 381 191 125 12 375
Fragments arranged by size:
381 375 254 191 125 12
BmrI ACTGGGnnnn_n'
Cuts at: 0 1311 1338
Size: 1311 27
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 39 1338
Size: 39 1299
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 261 757 1149 1338
Size: 261 496 392 189
Fragments arranged by size:
496 392 261 189
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 1338
Size: 211 1127
BsaAI yAC'GTr
Cuts at: 0 72 1338
Size: 72 1266
BsaHI Gr'CG_yC
Cuts at: 0 152 209 716 744 765 833 842 914
Size: 152 57 507 28 21 68 9 72
Cuts at: 914 934 1338
Size: 20 404
Fragments arranged by size:
507 404 152 72 68 57 28 21
20 9
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 538 568 1338
Size: 538 30 770
Fragments arranged by size:
770 538 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 472 1338
Size: 472 866
BseYI C'CCAG_C
Cuts at: 0 26 405 573 1338
Size: 26 379 168 765
Fragments arranged by size:
765 379 168 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 499 1338
Size: 364 135 839
Fragments arranged by size:
839 364 135
BsiEI CG_ry'CG
Cuts at: 0 751 1006 1109 1338
Size: 751 255 103 229
Fragments arranged by size:
751 255 229 103
BsiHKAI G_wGCw'C
Cuts at: 0 227 395 806 1338
Size: 227 168 411 532
Fragments arranged by size:
532 411 227 168
BsmI GAATG_Cn'
Cuts at: 0 322 1338
Size: 322 1016
BsmBI CGTCTCn'nnnn_
Cuts at: 0 107 982 1338
Size: 107 875 356
Fragments arranged by size:
875 356 107
Bsp1286I G_dGCh'C
Cuts at: 0 227 254 395 635 806 826 951 963
Size: 227 27 141 240 171 20 125 12
Cuts at: 963 1033 1338
Size: 70 305
Fragments arranged by size:
305 240 227 171 141 125 70 27
20 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 210 386 872 1163 1338
Size: 210 176 486 291 175
Fragments arranged by size:
486 291 210 176 175
BsrBI CCG'CTC
Cuts at: 0 23 1338
Size: 23 1315
BsrFI r'CCGG_y
Cuts at: 0 109 258 541 746 750 1117 1129 1235
Size: 109 149 283 205 4 367 12 106
Cuts at: 1235 1311 1338
Size: 76 27
Fragments arranged by size:
367 283 205 149 109 106 76 27
12 4
BsrGI T'GTAC_A
Cuts at: 0 602 1316 1338
Size: 602 714 22
Fragments arranged by size:
714 602 22
BssHII G'CGCG_C
Cuts at: 0 281 346 1338
Size: 281 65 992
Fragments arranged by size:
992 281 65
BssSI C'ACGA_G
Cuts at: 0 847 1338
Size: 847 491
BtgI C'CryG_G
Cuts at: 0 517 639 663 827 1000 1338
Size: 517 122 24 164 173 338
Fragments arranged by size:
517 338 173 164 122 24
EaeI y'GGCC_r
Cuts at: 0 359 543 748 1003 1035 1086 1106 1338
Size: 359 184 205 255 32 51 20 232
Fragments arranged by size:
359 255 232 205 184 51 32 20
EagI C'GGCC_G
Cuts at: 0 748 1003 1106 1338
Size: 748 255 103 232
Fragments arranged by size:
748 255 232 103
EarI CTCTTCn'nnn_
Cuts at: 0 523 1338
Size: 523 815
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 184 193 1338
Size: 184 9 1145
Fragments arranged by size:
1145 184 9
EcoICRI GAG'CTC
Cuts at: 0 393 804 1338
Size: 393 411 534
Fragments arranged by size:
534 411 393
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 261 632 751 757 765 1149 1338
Size: 261 371 119 6 8 384 189
Fragments arranged by size:
384 371 261 189 119 8 6
EcoO109I rG'GnC_Cy
Cuts at: 0 120 577 994 1338
Size: 120 457 417 344
Fragments arranged by size:
457 417 344 120
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 465 637 1338
Size: 465 172 701
Fragments arranged by size:
701 465 172
HaeII r_GCGC'y
Cuts at: 0 155 189 423 471 536 719 768 917
Size: 155 34 234 48 65 183 49 149
Cuts at: 917 1100 1338
Size: 183 238
Fragments arranged by size:
238 234 183 183 155 149 65 49
48 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 70 102 199 231 544 576 1338
Size: 70 32 97 32 313 32 762
Fragments arranged by size:
762 313 97 70 32 32 32
KasI G'GCGC_C
Cuts at: 0 151 715 764 913 1338
Size: 151 564 49 149 425
Fragments arranged by size:
564 425 151 149 49
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 581 1338
Size: 581 757
MslI CAynn'nnrTG
Cuts at: 0 259 638 956 1338
Size: 259 379 318 382
Fragments arranged by size:
382 379 318 259
MspA1I CmG'CkG
Cuts at: 0 761 1085 1338
Size: 761 324 253
Fragments arranged by size:
761 324 253
NaeI GCC'GGC
Cuts at: 0 111 543 748 1119 1131 1237 1338
Size: 111 432 205 371 12 106 101
Fragments arranged by size:
432 371 205 111 106 101 12
NarI GG'CG_CC
Cuts at: 0 152 716 765 914 1338
Size: 152 564 49 149 424
Fragments arranged by size:
564 424 152 149 49
NcoI C'CATG_G
Cuts at: 0 639 827 1338
Size: 639 188 511
Fragments arranged by size:
639 511 188
NgoMIV G'CCGG_C
Cuts at: 0 109 541 746 1117 1129 1235 1338
Size: 109 432 205 371 12 106 103
Fragments arranged by size:
432 371 205 109 106 103 12
NspI r_CATG'y
Cuts at: 0 304 1338
Size: 304 1034
PasI CC'CwG_GG
Cuts at: 0 870 1338
Size: 870 468
PflMI CCAn_nnn'nTGG
Cuts at: 0 1301 1338
Size: 1301 37
PmlI CAC'GTG
Cuts at: 0 72 1338
Size: 72 1266
PpuMI rG'GwC_Cy
Cuts at: 0 577 994 1338
Size: 577 417 344
Fragments arranged by size:
577 417 344
PshAI GACnn'nnGTC
Cuts at: 0 242 1338
Size: 242 1096
PspOMI G'GGCC_C
Cuts at: 0 947 1338
Size: 947 391
PstI C_TGCA'G
Cuts at: 0 40 707 1084 1160 1338
Size: 40 667 377 76 178
Fragments arranged by size:
667 377 178 76 40
SacI G_AGCT'C
Cuts at: 0 395 806 1338
Size: 395 411 532
Fragments arranged by size:
532 411 395
SanDI GG'GwC_CC
Cuts at: 0 577 994 1338
Size: 577 417 344
Fragments arranged by size:
577 417 344
SapI GCTCTTCn'nnn_
Cuts at: 0 523 1338
Size: 523 815
SbfI CC_TGCA'GG
Cuts at: 0 1160 1338
Size: 1160 178
SfcI C'TryA_G
Cuts at: 0 36 703 1080 1156 1338
Size: 36 667 377 76 182
Fragments arranged by size:
667 377 182 76 36
SfoI GGC'GCC
Cuts at: 0 153 717 766 915 1338
Size: 153 564 49 149 423
Fragments arranged by size:
564 423 153 149 49
SmaI CCC'GGG
Cuts at: 0 157 1338
Size: 157 1181
SmlI C'TyrA_G
Cuts at: 0 226 1338
Size: 226 1112
StuI AGG'CCT
Cuts at: 0 904 1162 1338
Size: 904 258 176
Fragments arranged by size:
904 258 176
StyI C'CwwG_G
Cuts at: 0 639 827 1338
Size: 639 188 511
Fragments arranged by size:
639 511 188
TatI w'GTAC_w
Cuts at: 0 602 1316 1338
Size: 602 714 22
Fragments arranged by size:
714 602 22
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 538 570 1338
Size: 538 32 768
Fragments arranged by size:
768 538 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 69 1338
Size: 69 1269
XmaI C'CCGG_G
Cuts at: 0 155 1338
Size: 155 1183
XmnI GAAnn'nnTTC
Cuts at: 0 322 1338
Size: 322 1016
ZraI GAC'GTC
Cuts at: 0 843 1338
Size: 843 495
Enzymes that do cut:
AarI AatII AcuI AfeI AhdI AleI AlwNI ApaI
AscI AvaI BanI BanII BbeI BglI BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaAI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrBI BsrFI BsrGI BssHII BssSI BtgI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I
KasI MmeI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PflMI PmlI PpuMI PshAI PspOMI PstI
SacI SanDI SapI SbfI SfcI SfoI SmaI SmlI
StuI StyI TatI TstI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AflIII AgeI AlfI AloI
ApaLI ApoI AseI AsiSI AvrII BaeI BamHI BbsI
BbvCI BcgI BciVI BclI BfrBI BglII BmgBI BmtI
Bpu10I BsaI BsaBI BsaWI BsiWI BspEI BspHI BsrDI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EcoNI EcoRI
EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI MscI NdeI NheI NotI NruI NsiI
PacI PciI PfoI PmeI PpiI PsiI PspXI PsrI
PvuI PvuII RsrII SacII SalI ScaI SexAI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
TaqII Tth111I XbaI XhoI