Restriction map for
pF1KB7749
(Linear) (Six-base) MAPSORT of: KIBB7749.seq Check: 3025 from: 1 to: 1329
KIBB7749.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
Acc65I G'GTAC_C
Cuts at: 0 907 1329
Size: 907 422
AfeI AGC'GCT
Cuts at: 0 982 1329
Size: 982 347
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 778 820 1329
Size: 778 42 509
Fragments arranged by size:
778 509 42
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 610 642 1329
Size: 610 32 687
Fragments arranged by size:
687 610 32
ApoI r'AATT_y
Cuts at: 0 1298 1329
Size: 1298 31
AvaI C'yCGr_G
Cuts at: 0 1176 1329
Size: 1176 153
BanI G'GyrC_C
Cuts at: 0 118 907 1012 1180 1198 1329
Size: 118 789 105 168 18 131
Fragments arranged by size:
789 168 131 118 105 18
BanII G_rGCy'C
Cuts at: 0 430 553 872 1121 1329
Size: 430 123 319 249 208
Fragments arranged by size:
430 319 249 208 123
BbeI G_GCGC'C
Cuts at: 0 122 1184 1329
Size: 122 1062 145
Fragments arranged by size:
1062 145 122
BbsI GAAGACnn'nnnn_
Cuts at: 0 1086 1329
Size: 1086 243
BbvCI CC'TCA_GC
Cuts at: 0 314 326 1329
Size: 314 12 1003
Fragments arranged by size:
1003 314 12
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 128 162 1329
Size: 128 34 1167
Fragments arranged by size:
1167 128 34
BfrBI ATG'CAT
Cuts at: 0 840 1329
Size: 840 489
BglI GCCn_nnn'nGGC
Cuts at: 0 418 1049 1329
Size: 418 631 280
Fragments arranged by size:
631 418 280
BglII A'GATC_T
Cuts at: 0 707 1329
Size: 707 622
Bme1580I G_kGCm'C
Cuts at: 0 809 1015 1329
Size: 809 206 314
Fragments arranged by size:
809 314 206
BmrI ACTGGGnnnn_n'
Cuts at: 0 867 1329
Size: 867 462
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 150 501 637 1329
Size: 139 11 351 136 692
Fragments arranged by size:
692 351 139 136 11
Bpu10I CC'TnA_GC
Cuts at: 0 223 314 326 522 1329
Size: 223 91 12 196 807
Fragments arranged by size:
807 223 196 91 12
BsaBI GATnn'nnATC
Cuts at: 0 706 1329
Size: 706 623
BsaHI Gr'CG_yC
Cuts at: 0 103 119 1181 1329
Size: 103 16 1062 148
Fragments arranged by size:
1062 148 103 16
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 610 640 1329
Size: 610 30 689
Fragments arranged by size:
689 610 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 371 1329
Size: 371 958
BseYI C'CCAG_C
Cuts at: 0 949 1209 1329
Size: 949 260 120
Fragments arranged by size:
949 260 120
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 320 1329
Size: 253 67 1009
Fragments arranged by size:
1009 253 67
BsiEI CG_ry'CG
Cuts at: 0 655 1329
Size: 655 674
BsmI GAATG_Cn'
Cuts at: 0 393 840 1329
Size: 393 447 489
Fragments arranged by size:
489 447 393
Bsp1286I G_dGCh'C
Cuts at: 0 430 553 809 872 1015 1121 1329
Size: 430 123 256 63 143 106 208
Fragments arranged by size:
430 256 208 143 123 106 63
BsrFI r'CCGG_y
Cuts at: 0 650 817 1273 1329
Size: 650 167 456 56
Fragments arranged by size:
650 456 167 56
BssHII G'CGCG_C
Cuts at: 0 576 578 580 599 1329
Size: 576 2 2 19 730
Fragments arranged by size:
730 576 19 2 2
BssSI C'ACGA_G
Cuts at: 0 594 1329
Size: 594 735
BstYI r'GATC_y
Cuts at: 0 707 1329
Size: 707 622
BtgI C'CryG_G
Cuts at: 0 1184 1227 1329
Size: 1184 43 102
Fragments arranged by size:
1184 102 43
EaeI y'GGCC_r
Cuts at: 0 419 452 652 671 1218 1275 1329
Size: 419 33 200 19 547 57 54
Fragments arranged by size:
547 419 200 57 54 33 19
EagI C'GGCC_G
Cuts at: 0 652 1329
Size: 652 677
EarI CTCTTCn'nnn_
Cuts at: 0 372 1329
Size: 372 957
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 96 1329
Size: 96 1233
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 150 501 637 1329
Size: 139 11 351 136 692
Fragments arranged by size:
692 351 139 136 11
EcoO109I rG'GnC_Cy
Cuts at: 0 1067 1237 1329
Size: 1067 170 92
Fragments arranged by size:
1067 170 92
EcoRI G'AATT_C
Cuts at: 0 1298 1329
Size: 1298 31
FspI TGC'GCA
Cuts at: 0 217 957 1329
Size: 217 740 372
Fragments arranged by size:
740 372 217
HaeII r_GCGC'y
Cuts at: 0 122 173 230 699 984 1184 1329
Size: 122 51 57 469 285 200 145
Fragments arranged by size:
469 285 200 145 122 57 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 660 692 859 891 1329
Size: 660 32 167 32 438
Fragments arranged by size:
660 438 167 32 32
HincII GTy'rAC
Cuts at: 0 852 1329
Size: 852 477
KasI G'GCGC_C
Cuts at: 0 118 1180 1329
Size: 118 1062 149
Fragments arranged by size:
1062 149 118
KpnI G_GTAC'C
Cuts at: 0 911 1329
Size: 911 418
MscI TGG'CCA
Cuts at: 0 454 673 1329
Size: 454 219 656
Fragments arranged by size:
656 454 219
MslI CAynn'nnrTG
Cuts at: 0 1037 1329
Size: 1037 292
MspA1I CmG'CkG
Cuts at: 0 606 953 1209 1217 1329
Size: 606 347 256 8 112
Fragments arranged by size:
606 347 256 112 8
NaeI GCC'GGC
Cuts at: 0 652 1329
Size: 652 677
NarI GG'CG_CC
Cuts at: 0 119 1181 1329
Size: 119 1062 148
Fragments arranged by size:
1062 148 119
NcoI C'CATG_G
Cuts at: 0 1184 1329
Size: 1184 145
NgoMIV G'CCGG_C
Cuts at: 0 650 1329
Size: 650 679
NsiI A_TGCA'T
Cuts at: 0 842 1329
Size: 842 487
NspI r_CATG'y
Cuts at: 0 765 1329
Size: 765 564
PasI CC'CwG_GG
Cuts at: 0 903 1329
Size: 903 426
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 610 642 1329
Size: 610 32 687
Fragments arranged by size:
687 610 32
PpuMI rG'GwC_Cy
Cuts at: 0 1067 1329
Size: 1067 262
PsiI TTA'TAA
Cuts at: 0 77 1329
Size: 77 1252
PstI C_TGCA'G
Cuts at: 0 1049 1329
Size: 1049 280
PvuII CAG'CTG
Cuts at: 0 606 953 1329
Size: 606 347 376
Fragments arranged by size:
606 376 347
SanDI GG'GwC_CC
Cuts at: 0 1067 1329
Size: 1067 262
SbfI CC_TGCA'GG
Cuts at: 0 1049 1329
Size: 1049 280
SfcI C'TryA_G
Cuts at: 0 1045 1329
Size: 1045 284
SfoI GGC'GCC
Cuts at: 0 120 1182 1329
Size: 120 1062 147
Fragments arranged by size:
1062 147 120
SphI G_CATG'C
Cuts at: 0 765 1329
Size: 765 564
StyI C'CwwG_G
Cuts at: 0 185 1184 1329
Size: 185 999 145
Fragments arranged by size:
999 185 145
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 459 1326 1329
Size: 459 867 3
Fragments arranged by size:
867 459 3
TatI w'GTAC_w
Cuts at: 0 734 1329
Size: 734 595
XmnI GAAnn'nnTTC
Cuts at: 0 625 1329
Size: 625 704
Enzymes that do cut:
Acc65I AfeI AlfI AloI ApoI AvaI BanI BanII
BbeI BbsI BbvCI BcgI BfrBI BglI BglII Bme1580I
BmrI BpmI Bpu10I BsaBI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsmI Bsp1286I BsrFI BssHII BssSI BstYI
BtgI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII KasI KpnI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI
PpiI PpuMI PsiI PstI PvuII SanDI SbfI SfcI
SfoI SphI StyI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AflII AflIII AgeI
AhdI AleI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BciVI BclI BlpI BmgBI BmtI
BplI BpuEI BsaI BsaAI BsaWI BsiHKAI BsiWI BsmBI
BspEI BspHI BspMI BsrBI BsrDI BsrGI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EcoICRI EcoNI EcoRV FalI FseI
FspAI HindIII HpaI MfeI MluI MmeI NdeI NheI
NotI NruI PacI PciI PflMI PfoI PmeI PmlI
PshAI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SapI ScaI SexAI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SrfI SspI StuI SwaI TstI
Tth111I XbaI XcmI XhoI XmaI ZraI