Restriction map for
pF1KB7741
(Linear) (Six-base) MAPSORT of: KIBB7741.seq Check: 856 from: 1 to: 1308
KIBB7741.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 1308
Size: 93 1215
ApaLI G'TGCA_C
Cuts at: 0 467 1308
Size: 467 841
ApoI r'AATT_y
Cuts at: 0 722 1308
Size: 722 586
BanII G_rGCy'C
Cuts at: 0 369 993 1300 1308
Size: 369 624 307 8
Fragments arranged by size:
624 369 307 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 321 1068 1152 1308
Size: 321 747 84 156
Fragments arranged by size:
747 321 156 84
BfrBI ATG'CAT
Cuts at: 0 654 1308
Size: 654 654
BglII A'GATC_T
Cuts at: 0 820 1308
Size: 820 488
Bme1580I G_kGCm'C
Cuts at: 0 471 1308
Size: 471 837
BmgBI CAC'GTC
Cuts at: 0 968 1308
Size: 968 340
BmrI ACTGGGnnnn_n'
Cuts at: 0 452 704 1043 1308
Size: 452 252 339 265
Fragments arranged by size:
452 339 265 252
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 940 1308
Size: 940 368
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 680 764 853 1019 1187 1308
Size: 311 369 84 89 166 168 121
Fragments arranged by size:
369 311 168 166 121 89 84
Bpu10I CC'TnA_GC
Cuts at: 0 1294 1308
Size: 1294 14
BsaI GGTCTCn'nnnn_
Cuts at: 0 492 1308
Size: 492 816
BsaHI Gr'CG_yC
Cuts at: 0 415 1308
Size: 415 893
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 61 1308
Size: 61 1247
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1152 1308
Size: 1152 156
BsiEI CG_ry'CG
Cuts at: 0 128 1308
Size: 128 1180
BsiHKAI G_wGCw'C
Cuts at: 0 369 471 993 1308
Size: 369 102 522 315
Fragments arranged by size:
522 369 315 102
BsmI GAATG_Cn'
Cuts at: 0 514 1308
Size: 514 794
BsmBI CGTCTCn'nnnn_
Cuts at: 0 406 1308
Size: 406 902
Bsp1286I G_dGCh'C
Cuts at: 0 369 471 993 1300 1308
Size: 369 102 522 307 8
Fragments arranged by size:
522 369 307 102 8
BspHI T'CATG_A
Cuts at: 0 1233 1308
Size: 1233 75
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 784 1308
Size: 784 524
BsrGI T'GTAC_A
Cuts at: 0 1102 1308
Size: 1102 206
BstAPI GCAn_nnn'nTGC
Cuts at: 0 518 1308
Size: 518 790
BstBI TT'CG_AA
Cuts at: 0 636 1059 1308
Size: 636 423 249
Fragments arranged by size:
636 423 249
BstEII G'GTnAC_C
Cuts at: 0 796 1308
Size: 796 512
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1270 1308
Size: 1270 38
BstYI r'GATC_y
Cuts at: 0 820 1308
Size: 820 488
ClaI AT'CG_AT
Cuts at: 0 701 1308
Size: 701 607
EaeI y'GGCC_r
Cuts at: 0 1250 1308
Size: 1250 58
EcoICRI GAG'CTC
Cuts at: 0 367 991 1308
Size: 367 624 317
Fragments arranged by size:
624 367 317
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 311 680 764 853 1019 1187 1308
Size: 93 218 369 84 89 166 168 121
Fragments arranged by size:
369 218 168 166 121 93 89 84
EcoO109I rG'GnC_Cy
Cuts at: 0 584 1308
Size: 584 724
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 108 1308
Size: 108 1200
MslI CAynn'nnrTG
Cuts at: 0 306 896 1098 1308
Size: 306 590 202 210
Fragments arranged by size:
590 306 210 202
NdeI CA'TA_TG
Cuts at: 0 1095 1308
Size: 1095 213
NsiI A_TGCA'T
Cuts at: 0 656 1308
Size: 656 652
PflMI CCAn_nnn'nTGG
Cuts at: 0 174 1308
Size: 174 1134
PfoI T'CCnGG_A
Cuts at: 0 347 1308
Size: 347 961
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 106 138 1308
Size: 106 32 1170
Fragments arranged by size:
1170 106 32
PpuMI rG'GwC_Cy
Cuts at: 0 584 1308
Size: 584 724
SacI G_AGCT'C
Cuts at: 0 369 993 1308
Size: 369 624 315
Fragments arranged by size:
624 369 315
SfcI C'TryA_G
Cuts at: 0 84 1308
Size: 84 1224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 55 114 1115 1308
Size: 55 59 1001 193
Fragments arranged by size:
1001 193 59 55
TatI w'GTAC_w
Cuts at: 0 1102 1308
Size: 1102 206
XmnI GAAnn'nnTTC
Cuts at: 0 328 1308
Size: 328 980
Enzymes that do cut:
AcuI ApaLI ApoI BanII BbsI BfrBI BglII Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BsaI BsaHI BseRI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrGI BstAPI BstBI BstEII BstXI BstYI ClaI EaeI
EcoICRI Eco57MI EcoO109I MmeI MslI NdeI NsiI PflMI
PfoI PpiI PpuMI SacI SfcI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BbvCI BcgI BciVI BclI BglI BlpI BmtI BpuEI
BsaAI BsaBI BsaWI BsaXI BseYI BsiWI BspEI BsrBI
BsrDI BsrFI BssHII BssSI BstZ17I Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EagI EarI EciI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MscI MspA1I NaeI NarI NcoI NgoMIV NheI NotI
NruI NspI PacI PasI PciI PmeI PmlI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TstI Tth111I XbaI
XcmI XhoI XmaI ZraI