Restriction map for
pF1KB7732
(Linear) (Six-base) MAPSORT of: KIBB7732.seq Check: 6653 from: 1 to: 1446
KIBB7732.orf
With 182 enzymes: SgfI *
February 15, 2010 17:50 ..
AccI GT'mk_AC
Cuts at: 0 1313 1446
Size: 1313 133
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 453 705 808 1041 1446
Size: 105 348 252 103 233 405
Fragments arranged by size:
405 348 252 233 105 103
AfeI AGC'GCT
Cuts at: 0 1390 1446
Size: 1390 56
AflIII A'CryG_T
Cuts at: 0 620 1446
Size: 620 826
AgeI A'CCGG_T
Cuts at: 0 1316 1446
Size: 1316 130
ApoI r'AATT_y
Cuts at: 0 228 634 839 1446
Size: 228 406 205 607
Fragments arranged by size:
607 406 228 205
BanII G_rGCy'C
Cuts at: 0 364 1179 1446
Size: 364 815 267
Fragments arranged by size:
815 364 267
BbsI GAAGACnn'nnnn_
Cuts at: 0 39 265 1382 1446
Size: 39 226 1117 64
Fragments arranged by size:
1117 226 64 39
BbvCI CC'TCA_GC
Cuts at: 0 497 1446
Size: 497 949
BglII A'GATC_T
Cuts at: 0 305 1446
Size: 305 1141
BlpI GC'TnA_GC
Cuts at: 0 510 1446
Size: 510 936
BmgBI CAC'GTC
Cuts at: 0 621 1446
Size: 621 825
BmrI ACTGGGnnnn_n'
Cuts at: 0 962 1446
Size: 962 484
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 622 1446
Size: 622 824
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 701 1121 1446
Size: 133 568 420 325
Fragments arranged by size:
568 420 325 133
Bpu10I CC'TnA_GC
Cuts at: 0 497 1446
Size: 497 949
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 326 1221 1446
Size: 326 895 225
Fragments arranged by size:
895 326 225
BsaI GGTCTCn'nnnn_
Cuts at: 0 1185 1446
Size: 1185 261
BsaBI GATnn'nnATC
Cuts at: 0 729 1446
Size: 729 717
BsaWI w'CCGG_w
Cuts at: 0 1316 1446
Size: 1316 130
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 175 205 1446
Size: 175 30 1241
Fragments arranged by size:
1241 175 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 70 73 1446
Size: 70 3 1373
Fragments arranged by size:
1373 70 3
BseYI C'CCAG_C
Cuts at: 0 63 1446
Size: 63 1383
BsiHKAI G_wGCw'C
Cuts at: 0 364 1446
Size: 364 1082
Bsp1286I G_dGCh'C
Cuts at: 0 364 1179 1446
Size: 364 815 267
Fragments arranged by size:
815 364 267
BspHI T'CATG_A
Cuts at: 0 810 1446
Size: 810 636
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 137 1446
Size: 137 1309
BsrDI GCAATG_nn'
Cuts at: 0 747 1263 1446
Size: 747 516 183
Fragments arranged by size:
747 516 183
BsrFI r'CCGG_y
Cuts at: 0 1316 1446
Size: 1316 130
BstYI r'GATC_y
Cuts at: 0 242 305 1446
Size: 242 63 1141
Fragments arranged by size:
1141 242 63
BstZ17I GTA'TAC
Cuts at: 0 1314 1446
Size: 1314 132
BtsI GCAGTG_nn'
Cuts at: 0 26 47 1446
Size: 26 21 1399
Fragments arranged by size:
1399 26 21
DraI TTT'AAA
Cuts at: 0 1436 1446
Size: 1436 10
EarI CTCTTCn'nnn_
Cuts at: 0 1045 1129 1213 1446
Size: 1045 84 84 233
Fragments arranged by size:
1045 233 84 84
EcoICRI GAG'CTC
Cuts at: 0 362 1446
Size: 362 1084
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 133 453 701 705 808 1041 1121
Size: 105 28 320 248 4 103 233 80
Cuts at: 1121 1446
Size: 325
Fragments arranged by size:
325 320 248 233 105 103 80 28
4
EcoO109I rG'GnC_Cy
Cuts at: 0 800 1446
Size: 800 646
EcoRI G'AATT_C
Cuts at: 0 228 634 839 1446
Size: 228 406 205 607
Fragments arranged by size:
607 406 228 205
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1419 1446
Size: 1419 27
HaeII r_GCGC'y
Cuts at: 0 1392 1404 1446
Size: 1392 12 42
Fragments arranged by size:
1392 42 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 227 259 376 408 636 668 876 908
Size: 227 32 117 32 228 32 208 32
Cuts at: 908 1446
Size: 538
Fragments arranged by size:
538 228 227 208 117 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 549 1306 1446
Size: 549 757 140
Fragments arranged by size:
757 549 140
MfeI C'AATT_G
Cuts at: 0 444 1446
Size: 444 1002
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 270 1446
Size: 270 1176
MslI CAynn'nnrTG
Cuts at: 0 530 864 1116 1284 1368 1446
Size: 530 334 252 168 84 78
Fragments arranged by size:
530 334 252 168 84 78
NspI r_CATG'y
Cuts at: 0 794 1446
Size: 794 652
PsiI TTA'TAA
Cuts at: 0 779 1199 1446
Size: 779 420 247
Fragments arranged by size:
779 420 247
SacI G_AGCT'C
Cuts at: 0 364 1446
Size: 364 1082
SapI GCTCTTCn'nnn_
Cuts at: 0 1045 1129 1213 1446
Size: 1045 84 84 233
Fragments arranged by size:
1045 233 84 84
SmlI C'TyrA_G
Cuts at: 0 341 1236 1446
Size: 341 895 210
Fragments arranged by size:
895 341 210
StuI AGG'CCT
Cuts at: 0 339 1234 1446
Size: 339 895 212
Fragments arranged by size:
895 339 212
StyI C'CwwG_G
Cuts at: 0 11 1446
Size: 11 1435
Tth111I GACn'n_nGTC
Cuts at: 0 6 1446
Size: 6 1440
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 287 1446
Size: 287 1159
XmnI GAAnn'nnTTC
Cuts at: 0 691 859 1054 1446
Size: 691 168 195 392
Fragments arranged by size:
691 392 195 168
Enzymes that do cut:
AccI AcuI AfeI AflIII AgeI ApoI BanII BbsI
BbvCI BglII BlpI BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaBI BsaWI BsaXI BseRI BseYI BsiHKAI
Bsp1286I BspHI BspMI BsrDI BsrFI BstYI BstZ17I BtsI
DraI EarI EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII
Hin4I HindIII MfeI MmeI MslI NspI PsiI SacI
SapI SmlI StuI StyI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AhdI AleI AlfI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BcgI BciVI BclI
BfrBI BglI Bme1580I BmtI BsaAI BsaHI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BsrBI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI Bsu36I BtgI BtgZI ClaI
CspCI DraIII DrdI EaeI EagI EciI EcoNI EcoRV
FseI FspI FspAI HincII HpaI KasI KpnI MluI
MscI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacII SalI SanDI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI
TstI XbaI XhoI XmaI ZraI