Restriction map for
pF1KB7731
(Linear) (Six-base) MAPSORT of: KIBB7731.seq Check: 1071 from: 1 to: 1296
KIBB7731.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AfeI AGC'GCT
Cuts at: 0 982 1296
Size: 982 314
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 74 286 982 1296
Size: 74 212 696 314
Fragments arranged by size:
696 314 212 74
AlwNI CAG_nnn'CTG
Cuts at: 0 27 196 962 1296
Size: 27 169 766 334
Fragments arranged by size:
766 334 169 27
ApaI G_GGCC'C
Cuts at: 0 440 1296
Size: 440 856
ApaLI G'TGCA_C
Cuts at: 0 42 1296
Size: 42 1254
ApoI r'AATT_y
Cuts at: 0 634 1296
Size: 634 662
BanI G'GyrC_C
Cuts at: 0 823 948 1098 1296
Size: 823 125 150 198
Fragments arranged by size:
823 198 150 125
BanII G_rGCy'C
Cuts at: 0 217 234 440 565 1007 1296
Size: 217 17 206 125 442 289
Fragments arranged by size:
442 289 217 206 125 17
BbeI G_GCGC'C
Cuts at: 0 827 952 1102 1296
Size: 827 125 150 194
Fragments arranged by size:
827 194 150 125
BbsI GAAGACnn'nnnn_
Cuts at: 0 1110 1296
Size: 1110 186
BbvCI CC'TCA_GC
Cuts at: 0 456 1296
Size: 456 840
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1068 1102 1296
Size: 1068 34 194
Fragments arranged by size:
1068 194 34
BglI GCCn_nnn'nGGC
Cuts at: 0 569 946 1296
Size: 569 377 350
Fragments arranged by size:
569 377 350
BglII A'GATC_T
Cuts at: 0 476 716 1233 1296
Size: 476 240 517 63
Fragments arranged by size:
517 476 240 63
Bme1580I G_kGCm'C
Cuts at: 0 46 283 440 869 1296
Size: 46 237 157 429 427
Fragments arranged by size:
429 427 237 157 46
BmrI ACTGGGnnnn_n'
Cuts at: 0 293 1296
Size: 293 1003
BmtI G_CTAG'C
Cuts at: 0 791 1296
Size: 791 505
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 271 1296
Size: 271 1025
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 422 1296
Size: 49 373 874
Fragments arranged by size:
874 373 49
Bpu10I CC'TnA_GC
Cuts at: 0 456 800 1296
Size: 456 344 496
Fragments arranged by size:
496 456 344
BsaAI yAC'GTr
Cuts at: 0 837 1071 1296
Size: 837 234 225
Fragments arranged by size:
837 234 225
BsaBI GATnn'nnATC
Cuts at: 0 715 1296
Size: 715 581
BsaHI Gr'CG_yC
Cuts at: 0 824 949 1099 1296
Size: 824 125 150 197
Fragments arranged by size:
824 197 150 125
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 395 425 1296
Size: 395 30 871
Fragments arranged by size:
871 395 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 350 444 1296
Size: 350 94 852
Fragments arranged by size:
852 350 94
BseYI C'CCAG_C
Cuts at: 0 234 573 1296
Size: 234 339 723
Fragments arranged by size:
723 339 234
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 760 1296
Size: 760 536
BsiEI CG_ry'CG
Cuts at: 0 277 664 1296
Size: 277 387 632
Fragments arranged by size:
632 387 277
BsiHKAI G_wGCw'C
Cuts at: 0 46 217 1296
Size: 46 171 1079
Fragments arranged by size:
1079 171 46
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1259 1296
Size: 1259 37
Bsp1286I G_dGCh'C
Cuts at: 0 46 217 234 283 440 565 869 1007
Size: 46 171 17 49 157 125 304 138
Cuts at: 1007 1296
Size: 289
Fragments arranged by size:
304 289 171 157 138 125 49 46
17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 695 1058 1296
Size: 695 363 238
Fragments arranged by size:
695 363 238
BsrBI CCG'CTC
Cuts at: 0 816 1296
Size: 816 480
BsrFI r'CCGG_y
Cuts at: 0 659 731 783 1296
Size: 659 72 52 513
Fragments arranged by size:
659 513 72 52
BssHII G'CGCG_C
Cuts at: 0 608 1296
Size: 608 688
BssSI C'ACGA_G
Cuts at: 0 603 1296
Size: 603 693
BstXI CCAn_nnnn'nTGG
Cuts at: 0 174 322 1201 1296
Size: 174 148 879 95
Fragments arranged by size:
879 174 148 95
BstYI r'GATC_y
Cuts at: 0 476 716 1233 1296
Size: 476 240 517 63
Fragments arranged by size:
517 476 240 63
BtgI C'CryG_G
Cuts at: 0 1013 1094 1200 1296
Size: 1013 81 106 96
Fragments arranged by size:
1013 106 96 81
BtsI GCAGTG_nn'
Cuts at: 0 533 1296
Size: 533 763
EaeI y'GGCC_r
Cuts at: 0 274 661 680 831 849 935 1151 1296
Size: 274 387 19 151 18 86 216 145
Fragments arranged by size:
387 274 216 151 145 86 19 18
EagI C'GGCC_G
Cuts at: 0 274 661 1296
Size: 274 387 635
Fragments arranged by size:
635 387 274
EcoICRI GAG'CTC
Cuts at: 0 215 1296
Size: 215 1081
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 422 1296
Size: 49 373 874
Fragments arranged by size:
874 373 49
EcoO109I rG'GnC_Cy
Cuts at: 0 24 1296
Size: 24 1272
EcoRI G'AATT_C
Cuts at: 0 634 1296
Size: 634 662
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 495 1296
Size: 495 801
HaeII r_GCGC'y
Cuts at: 0 827 952 984 994 1046 1102 1296
Size: 827 125 32 10 52 56 194
Fragments arranged by size:
827 194 125 56 52 32 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 874 906 1296
Size: 874 32 390
Fragments arranged by size:
874 390 32
KasI G'GCGC_C
Cuts at: 0 823 948 1098 1296
Size: 823 125 150 198
Fragments arranged by size:
823 198 150 125
MscI TGG'CCA
Cuts at: 0 682 833 1296
Size: 682 151 463
Fragments arranged by size:
682 463 151
MslI CAynn'nnrTG
Cuts at: 0 1199 1296
Size: 1199 97
MspA1I CmG'CkG
Cuts at: 0 266 527 615 829 855 962 1296
Size: 266 261 88 214 26 107 334
Fragments arranged by size:
334 266 261 214 107 88 26
NaeI GCC'GGC
Cuts at: 0 661 785 1296
Size: 661 124 511
Fragments arranged by size:
661 511 124
NarI GG'CG_CC
Cuts at: 0 824 949 1099 1296
Size: 824 125 150 197
Fragments arranged by size:
824 197 150 125
NcoI C'CATG_G
Cuts at: 0 1013 1094 1200 1296
Size: 1013 81 106 96
Fragments arranged by size:
1013 106 96 81
NgoMIV G'CCGG_C
Cuts at: 0 659 783 1296
Size: 659 124 513
Fragments arranged by size:
659 513 124
NheI G'CTAG_C
Cuts at: 0 787 1296
Size: 787 509
NspI r_CATG'y
Cuts at: 0 273 1296
Size: 273 1023
PflMI CCAn_nnn'nTGG
Cuts at: 0 1014 1296
Size: 1014 282
PfoI T'CCnGG_A
Cuts at: 0 399 1296
Size: 399 897
PmlI CAC'GTG
Cuts at: 0 837 1296
Size: 837 459
PspOMI G'GGCC_C
Cuts at: 0 436 1296
Size: 436 860
PstI C_TGCA'G
Cuts at: 0 540 1055 1296
Size: 540 515 241
Fragments arranged by size:
540 515 241
PvuII CAG'CTG
Cuts at: 0 266 527 615 855 962 1296
Size: 266 261 88 240 107 334
Fragments arranged by size:
334 266 261 240 107 88
SacI G_AGCT'C
Cuts at: 0 217 1296
Size: 217 1079
SbfI CC_TGCA'GG
Cuts at: 0 1055 1296
Size: 1055 241
SfcI C'TryA_G
Cuts at: 0 99 126 536 1051 1164 1296
Size: 99 27 410 515 113 132
Fragments arranged by size:
515 410 132 113 99 27
SfoI GGC'GCC
Cuts at: 0 825 950 1100 1296
Size: 825 125 150 196
Fragments arranged by size:
825 196 150 125
SphI G_CATG'C
Cuts at: 0 273 1296
Size: 273 1023
StyI C'CwwG_G
Cuts at: 0 764 1013 1094 1200 1296
Size: 764 249 81 106 96
Fragments arranged by size:
764 249 106 96 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 405 1296
Size: 405 891
TatI w'GTAC_w
Cuts at: 0 743 1296
Size: 743 553
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 171 203 395 427 1296
Size: 171 32 192 32 869
Fragments arranged by size:
869 192 171 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 301 1296
Size: 301 995
Enzymes that do cut:
AfeI AlfI AlwNI ApaI ApaLI ApoI BanI BanII
BbeI BbsI BbvCI BcgI BglI BglII Bme1580I BmrI
BmtI BplI BpmI Bpu10I BsaAI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI
BsrBI BsrFI BssHII BssSI BstXI BstYI BtgI BtsI
EaeI EagI EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII
Hin4I KasI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PflMI PfoI PmlI PspOMI PstI
PvuII SacI SbfI SfcI SfoI SphI StyI TaqII
TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AloI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BciVI BclI BfrBI BlpI BmgBI
BpuEI BsaI BsaWI BsiWI BsmI BspEI BspHI BsrDI
BsrGI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EarI EciI EcoNI EcoRV
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI MmeI NdeI NotI NruI NsiI PacI PasI
PciI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI ScaI SexAI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI
SspI StuI SwaI Tth111I XbaI XhoI XmaI XmnI
ZraI