Restriction map for
pF1KB7729
(Linear) (Six-base) MAPSORT of: KIBB7729.seq Check: 3613 from: 1 to: 1293
KIBB7729.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 198 605 622 1293
Size: 122 76 407 17 671
Fragments arranged by size:
671 407 122 76 17
AflIII A'CryG_T
Cuts at: 0 1165 1234 1293
Size: 1165 69 59
Fragments arranged by size:
1165 69 59
AhdI GACnn_n'nnGTC
Cuts at: 0 147 1293
Size: 147 1146
AleI CACnn'nnGTG
Cuts at: 0 136 1293
Size: 136 1157
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1168 1293
Size: 1168 125
AlwNI CAG_nnn'CTG
Cuts at: 0 52 600 1293
Size: 52 548 693
Fragments arranged by size:
693 548 52
ApoI r'AATT_y
Cuts at: 0 410 471 761 991 1226 1293
Size: 410 61 290 230 235 67
Fragments arranged by size:
410 290 235 230 67 61
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1046 1079 1293
Size: 1046 33 214
Fragments arranged by size:
1046 214 33
BanII G_rGCy'C
Cuts at: 0 1190 1293
Size: 1190 103
BbsI GAAGACnn'nnnn_
Cuts at: 0 226 346 953 962 1293
Size: 226 120 607 9 331
Fragments arranged by size:
607 331 226 120 9
BfrBI ATG'CAT
Cuts at: 0 829 1102 1293
Size: 829 273 191
Fragments arranged by size:
829 273 191
BmrI ACTGGGnnnn_n'
Cuts at: 0 836 1293
Size: 836 457
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 8 1293
Size: 8 1285
BsaAI yAC'GTr
Cuts at: 0 1014 1293
Size: 1014 279
BsaWI w'CCGG_w
Cuts at: 0 570 1293
Size: 570 723
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 260 290 1293
Size: 260 30 1003
Fragments arranged by size:
1003 260 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 828 1198 1293
Size: 828 370 95
Fragments arranged by size:
828 370 95
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 437 1293
Size: 437 856
BsiHKAI G_wGCw'C
Cuts at: 0 380 1190 1293
Size: 380 810 103
Fragments arranged by size:
810 380 103
Bsp1286I G_dGCh'C
Cuts at: 0 380 1190 1293
Size: 380 810 103
Fragments arranged by size:
810 380 103
BspEI T'CCGG_A
Cuts at: 0 570 1293
Size: 570 723
BspHI T'CATG_A
Cuts at: 0 186 1293
Size: 186 1107
BsrDI GCAATG_nn'
Cuts at: 0 734 799 831 1293
Size: 734 65 32 462
Fragments arranged by size:
734 462 65 32
BstXI CCAn_nnnn'nTGG
Cuts at: 0 138 464 1222 1293
Size: 138 326 758 71
Fragments arranged by size:
758 326 138 71
BstYI r'GATC_y
Cuts at: 0 1204 1293
Size: 1204 89
BtgI C'CryG_G
Cuts at: 0 528 1293
Size: 528 765
BtsI GCAGTG_nn'
Cuts at: 0 1035 1293
Size: 1035 258
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 867 902 1293
Size: 867 35 391
Fragments arranged by size:
867 391 35
DraI TTT'AAA
Cuts at: 0 740 1293
Size: 740 553
EaeI y'GGCC_r
Cuts at: 0 368 1293
Size: 368 925
EarI CTCTTCn'nnn_
Cuts at: 0 788 1293
Size: 788 505
EcoICRI GAG'CTC
Cuts at: 0 1188 1293
Size: 1188 105
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 8 122 198 605 622 1293
Size: 8 114 76 407 17 671
Fragments arranged by size:
671 407 114 76 17 8
EcoRI G'AATT_C
Cuts at: 0 471 1226 1293
Size: 471 755 67
Fragments arranged by size:
755 471 67
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 133 165 265 297 1196 1228 1257 1289
Size: 133 32 100 32 899 32 29 32
Cuts at: 1289 1293
Size: 4
Fragments arranged by size:
899 133 100 32 32 32 32 29
4
HindIII A'AGCT_T
Cuts at: 0 93 1293
Size: 93 1200
MfeI C'AATT_G
Cuts at: 0 239 1293
Size: 239 1054
MscI TGG'CCA
Cuts at: 0 370 1293
Size: 370 923
MslI CAynn'nnrTG
Cuts at: 0 136 364 842 1293
Size: 136 228 478 451
Fragments arranged by size:
478 451 228 136
MspA1I CmG'CkG
Cuts at: 0 1201 1293
Size: 1201 92
NcoI C'CATG_G
Cuts at: 0 528 1293
Size: 528 765
NsiI A_TGCA'T
Cuts at: 0 831 1104 1293
Size: 831 273 189
Fragments arranged by size:
831 273 189
NspI r_CATG'y
Cuts at: 0 1169 1238 1293
Size: 1169 69 55
Fragments arranged by size:
1169 69 55
PciI A'CATG_T
Cuts at: 0 1165 1234 1293
Size: 1165 69 59
Fragments arranged by size:
1165 69 59
PflMI CCAn_nnn'nTGG
Cuts at: 0 13 286 1293
Size: 13 273 1007
Fragments arranged by size:
1007 273 13
PmlI CAC'GTG
Cuts at: 0 1014 1293
Size: 1014 279
PvuII CAG'CTG
Cuts at: 0 1201 1293
Size: 1201 92
SacI G_AGCT'C
Cuts at: 0 1190 1293
Size: 1190 103
ScaI AGT'ACT
Cuts at: 0 1257 1293
Size: 1257 36
SexAI A'CCwGG_T
Cuts at: 0 683 1293
Size: 683 610
SfcI C'TryA_G
Cuts at: 0 749 1293
Size: 749 544
StyI C'CwwG_G
Cuts at: 0 528 1293
Size: 528 765
TatI w'GTAC_w
Cuts at: 0 1255 1293
Size: 1255 38
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 260 292 1293
Size: 260 32 1001
Fragments arranged by size:
1001 260 32
XmnI GAAnn'nnTTC
Cuts at: 0 549 1037 1293
Size: 549 488 256
Fragments arranged by size:
549 488 256
Enzymes that do cut:
AcuI AflIII AhdI AleI AlfI AlwNI ApoI BaeI
BanII BbsI BfrBI BmrI BpmI BsaAI BsaWI BsaXI
BseRI BsgI BsiHKAI Bsp1286I BspEI BspHI BsrDI BstXI
BstYI BtgI BtsI CspCI DraI EaeI EarI EcoICRI
Eco57MI EcoRI Hin4I HindIII MfeI MscI MslI MspA1I
NcoI NsiI NspI PciI PflMI PmlI PvuII SacI
ScaI SexAI SfcI StyI TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BamHI BanI BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmtI BplI Bpu10I BpuEI
BsaI BsaBI BsaHI BseYI BsiEI BsiWI BsmI BsmBI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI DraIII DrdI EagI
EciI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI
HaeII HincII HpaI KasI KpnI MluI MmeI NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PasI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacII SalI SanDI SapI
SbfI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII Tth111I
XbaI XcmI XhoI XmaI ZraI