Restriction map for
pF1KB7726
(Linear) (Six-base) MAPSORT of: KIBB7726.seq Check: 3103 from: 1 to: 1440
KIBB7726.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1280 1440
Size: 1280 160
AatII G_ACGT'C
Cuts at: 0 119 1440
Size: 119 1321
AfeI AGC'GCT
Cuts at: 0 29 1440
Size: 29 1411
AleI CACnn'nnGTG
Cuts at: 0 1411 1440
Size: 1411 29
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 439 1440
Size: 439 1001
AlwNI CAG_nnn'CTG
Cuts at: 0 319 1440
Size: 319 1121
ApaI G_GGCC'C
Cuts at: 0 1439 1440
Size: 1439 1
ApaLI G'TGCA_C
Cuts at: 0 1048 1414 1440
Size: 1048 366 26
Fragments arranged by size:
1048 366 26
ApoI r'AATT_y
Cuts at: 0 898 1440
Size: 898 542
AvaI C'yCGr_G
Cuts at: 0 241 1429 1440
Size: 241 1188 11
Fragments arranged by size:
1188 241 11
BamHI G'GATC_C
Cuts at: 0 104 1440
Size: 104 1336
BanII G_rGCy'C
Cuts at: 0 810 879 1092 1439 1440
Size: 810 69 213 347 1
Fragments arranged by size:
810 347 213 69 1
BbsI GAAGACnn'nnnn_
Cuts at: 0 980 998 1440
Size: 980 18 442
Fragments arranged by size:
980 442 18
Bme1580I G_kGCm'C
Cuts at: 0 1052 1418 1439 1440
Size: 1052 366 21 1
Fragments arranged by size:
1052 366 21 1
BmgBI CAC'GTC
Cuts at: 0 69 228 1166 1440
Size: 69 159 938 274
Fragments arranged by size:
938 274 159 69
BmrI ACTGGGnnnn_n'
Cuts at: 0 539 677 1440
Size: 539 138 763
Fragments arranged by size:
763 539 138
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 684 794 1440
Size: 296 388 110 646
Fragments arranged by size:
646 388 296 110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 830 1267 1440
Size: 830 437 173
Fragments arranged by size:
830 437 173
BsaAI yAC'GTr
Cuts at: 0 366 1209 1440
Size: 366 843 231
Fragments arranged by size:
843 366 231
BsaHI Gr'CG_yC
Cuts at: 0 116 1440
Size: 116 1324
BsaWI w'CCGG_w
Cuts at: 0 1306 1440
Size: 1306 134
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 206 236 450 480 682 712 1440
Size: 206 30 214 30 202 30 728
Fragments arranged by size:
728 214 206 202 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 51 820 1440
Size: 51 769 620
Fragments arranged by size:
769 620 51
BseYI C'CCAG_C
Cuts at: 0 30 72 1440
Size: 30 42 1368
Fragments arranged by size:
1368 42 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 811 1440
Size: 305 506 629
Fragments arranged by size:
629 506 305
BsiEI CG_ry'CG
Cuts at: 0 341 1440
Size: 341 1099
BsiHKAI G_wGCw'C
Cuts at: 0 153 233 365 940 1052 1418 1440
Size: 153 80 132 575 112 366 22
Fragments arranged by size:
575 366 153 132 112 80 22
BsmBI CGTCTCn'nnnn_
Cuts at: 0 683 1156 1440
Size: 683 473 284
Fragments arranged by size:
683 473 284
Bsp1286I G_dGCh'C
Cuts at: 0 153 233 365 810 879 940 1052 1092
Size: 153 80 132 445 69 61 112 40
Cuts at: 1092 1418 1439 1440
Size: 326 21 1
Fragments arranged by size:
445 326 153 132 112 80 69 61
40 21 1
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1258 1280 1440
Size: 1258 22 160
Fragments arranged by size:
1258 160 22
BsrFI r'CCGG_y
Cuts at: 0 1001 1100 1440
Size: 1001 99 340
Fragments arranged by size:
1001 340 99
BsrGI T'GTAC_A
Cuts at: 0 287 1182 1440
Size: 287 895 258
Fragments arranged by size:
895 287 258
BstYI r'GATC_y
Cuts at: 0 104 1440
Size: 104 1336
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 153 1440
Size: 153 1287
BtsI GCAGTG_nn'
Cuts at: 0 183 317 738 1071 1323 1440
Size: 183 134 421 333 252 117
Fragments arranged by size:
421 333 252 183 134 117
EaeI y'GGCC_r
Cuts at: 0 338 1102 1440
Size: 338 764 338
Fragments arranged by size:
764 338 338
EagI C'GGCC_G
Cuts at: 0 338 1440
Size: 338 1102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1255 1440
Size: 1255 185
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 684 794 1440
Size: 296 388 110 646
Fragments arranged by size:
646 388 296 110
EcoNI CCTnn'n_nnAGG
Cuts at: 0 470 1440
Size: 470 970
EcoO109I rG'GnC_Cy
Cuts at: 0 247 538 742 1435 1440
Size: 247 291 204 693 5
Fragments arranged by size:
693 291 247 204 5
HaeII r_GCGC'y
Cuts at: 0 31 489 1440
Size: 31 458 951
Fragments arranged by size:
951 458 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 217 249 682 714 1440
Size: 217 32 433 32 726
Fragments arranged by size:
726 433 217 32 32
HincII GTy'rAC
Cuts at: 0 658 1363 1440
Size: 658 705 77
Fragments arranged by size:
705 658 77
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 799 1440
Size: 89 710 641
Fragments arranged by size:
710 641 89
MslI CAynn'nnrTG
Cuts at: 0 1411 1440
Size: 1411 29
MspA1I CmG'CkG
Cuts at: 0 908 996 1440
Size: 908 88 444
Fragments arranged by size:
908 444 88
NotI GC'GGCC_GC
Cuts at: 0 338 1440
Size: 338 1102
NspI r_CATG'y
Cuts at: 0 380 1440
Size: 380 1060
PmlI CAC'GTG
Cuts at: 0 366 1209 1440
Size: 366 843 231
Fragments arranged by size:
843 366 231
PpuMI rG'GwC_Cy
Cuts at: 0 247 742 1440
Size: 247 495 698
Fragments arranged by size:
698 495 247
PspOMI G'GGCC_C
Cuts at: 0 1435 1440
Size: 1435 5
PstI C_TGCA'G
Cuts at: 0 178 532 1277 1440
Size: 178 354 745 163
Fragments arranged by size:
745 354 178 163
PvuII CAG'CTG
Cuts at: 0 908 996 1440
Size: 908 88 444
Fragments arranged by size:
908 444 88
SanDI GG'GwC_CC
Cuts at: 0 247 1440
Size: 247 1193
SexAI A'CCwGG_T
Cuts at: 0 471 1440
Size: 471 969
SfcI C'TryA_G
Cuts at: 0 174 528 1273 1440
Size: 174 354 745 167
Fragments arranged by size:
745 354 174 167
SmaI CCC'GGG
Cuts at: 0 243 1440
Size: 243 1197
SmlI C'TyrA_G
Cuts at: 0 845 1282 1440
Size: 845 437 158
Fragments arranged by size:
845 437 158
SphI G_CATG'C
Cuts at: 0 380 1440
Size: 380 1060
StuI AGG'CCT
Cuts at: 0 571 616 697 1440
Size: 571 45 81 743
Fragments arranged by size:
743 571 81 45
StyI C'CwwG_G
Cuts at: 0 566 956 1440
Size: 566 390 484
Fragments arranged by size:
566 484 390
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 515 1440
Size: 515 925
TatI w'GTAC_w
Cuts at: 0 287 587 593 1182 1440
Size: 287 300 6 589 258
Fragments arranged by size:
589 300 287 258 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 206 238 314 346 1440
Size: 206 32 76 32 1094
Fragments arranged by size:
1094 206 76 32 32
XmaI C'CCGG_G
Cuts at: 0 241 1440
Size: 241 1199
ZraI GAC'GTC
Cuts at: 0 117 1440
Size: 117 1323
Enzymes that do cut:
AarI AatII AfeI AleI AlfI AlwNI ApaI ApaLI
ApoI AvaI BamHI BanII BbsI Bme1580I BmgBI BmrI
BpmI BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BsrGI
BstYI BtgZI BtsI EaeI EagI EciI Eco57MI EcoNI
EcoO109I HaeII Hin4I HincII MmeI MslI MspA1I NotI
NspI PmlI PpuMI PspOMI PstI PvuII SanDI SexAI
SfcI SmaI SmlI SphI StuI StyI TaqII TatI
TstI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AloI AscI AseI AsiSI AvrII BaeI BanI BbeI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
BmtI BplI Bpu10I BsaI BsaBI BsiWI BsmI BspEI
BspHI BsrBI BsrDI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraI DraIII
DrdI EarI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI MscI
NaeI NarI NcoI NdeI NgoMIV NheI NruI NsiI
PacI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI
SpeI SrfI SspI SwaI Tth111I XbaI XcmI XhoI
XmnI