Restriction map for
pF1KB7723
(Linear) (Six-base) MAPSORT of: KIBB7723.seq Check: 7998 from: 1 to: 1290
KIBB7723.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 697 1290
Size: 697 593
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 697 735 1220 1290
Size: 697 38 485 70
Fragments arranged by size:
697 485 70 38
AflIII A'CryG_T
Cuts at: 0 1061 1290
Size: 1061 229
AlwNI CAG_nnn'CTG
Cuts at: 0 201 418 427 436 1027 1054 1290
Size: 201 217 9 9 591 27 236
Fragments arranged by size:
591 236 217 201 27 9 9
ApaI G_GGCC'C
Cuts at: 0 82 389 1290
Size: 82 307 901
Fragments arranged by size:
901 307 82
AvaI C'yCGr_G
Cuts at: 0 132 388 1162 1290
Size: 132 256 774 128
Fragments arranged by size:
774 256 132 128
BanII G_rGCy'C
Cuts at: 0 82 313 389 1085 1290
Size: 82 231 76 696 205
Fragments arranged by size:
696 231 205 82 76
BbvCI CC'TCA_GC
Cuts at: 0 298 580 1290
Size: 298 282 710
Fragments arranged by size:
710 298 282
BglI GCCn_nnn'nGGC
Cuts at: 0 47 1290
Size: 47 1243
BlpI GC'TnA_GC
Cuts at: 0 405 1290
Size: 405 885
Bme1580I G_kGCm'C
Cuts at: 0 82 389 1290
Size: 82 307 901
Fragments arranged by size:
901 307 82
BmrI ACTGGGnnnn_n'
Cuts at: 0 25 1290
Size: 25 1265
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 14 547 1076 1290
Size: 14 533 529 214
Fragments arranged by size:
533 529 214 14
Bpu10I CC'TnA_GC
Cuts at: 0 298 580 1290
Size: 298 282 710
Fragments arranged by size:
710 298 282
BsaI GGTCTCn'nnnn_
Cuts at: 0 98 876 1290
Size: 98 778 414
Fragments arranged by size:
778 414 98
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 334 337 835 847 946 1290
Size: 334 3 498 12 99 344
Fragments arranged by size:
498 344 334 99 12 3
BseYI C'CCAG_C
Cuts at: 0 382 1290
Size: 382 908
BsiHKAI G_wGCw'C
Cuts at: 0 313 688 1290
Size: 313 375 602
Fragments arranged by size:
602 375 313
BsmI GAATG_Cn'
Cuts at: 0 302 1290
Size: 302 988
Bsp1286I G_dGCh'C
Cuts at: 0 82 313 389 688 1085 1290
Size: 82 231 76 299 397 205
Fragments arranged by size:
397 299 231 205 82 76
BspHI T'CATG_A
Cuts at: 0 569 1290
Size: 569 721
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 584 697 1290
Size: 584 113 593
Fragments arranged by size:
593 584 113
BsrFI r'CCGG_y
Cuts at: 0 327 1290
Size: 327 963
BsrGI T'GTAC_A
Cuts at: 0 1178 1290
Size: 1178 112
BstAPI GCAn_nnn'nTGC
Cuts at: 0 418 427 1290
Size: 418 9 863
Fragments arranged by size:
863 418 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 606 621 1290
Size: 606 15 669
Fragments arranged by size:
669 606 15
BstYI r'GATC_y
Cuts at: 0 500 1051 1290
Size: 500 551 239
Fragments arranged by size:
551 500 239
BtgI C'CryG_G
Cuts at: 0 605 1186 1290
Size: 605 581 104
Fragments arranged by size:
605 581 104
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 831 866 1290
Size: 831 35 424
Fragments arranged by size:
831 424 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 963 1290
Size: 963 327
EarI CTCTTCn'nnn_
Cuts at: 0 1239 1290
Size: 1239 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 116 1290
Size: 116 1174
EcoICRI GAG'CTC
Cuts at: 0 311 1290
Size: 311 979
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 14 547 697 735 1076 1220 1290
Size: 14 533 150 38 341 144 70
Fragments arranged by size:
533 341 150 144 70 38 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 205 1290
Size: 205 1085
EcoO109I rG'GnC_Cy
Cuts at: 0 48 78 79 129 771 1290
Size: 48 30 1 50 642 519
Fragments arranged by size:
642 519 50 48 30 1
HaeII r_GCGC'y
Cuts at: 0 662 744 1290
Size: 662 82 546
Fragments arranged by size:
662 546 82
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 899 1149 1290
Size: 899 250 141
Fragments arranged by size:
899 250 141
MslI CAynn'nnrTG
Cuts at: 0 667 859 1290
Size: 667 192 431
Fragments arranged by size:
667 431 192
MspA1I CmG'CkG
Cuts at: 0 342 418 427 675 1211 1290
Size: 342 76 9 248 536 79
Fragments arranged by size:
536 342 248 79 76 9
NspI r_CATG'y
Cuts at: 0 363 548 1065 1290
Size: 363 185 517 225
Fragments arranged by size:
517 363 225 185
PasI CC'CwG_GG
Cuts at: 0 975 1290
Size: 975 315
PciI A'CATG_T
Cuts at: 0 1061 1290
Size: 1061 229
PflMI CCAn_nnn'nTGG
Cuts at: 0 201 1290
Size: 201 1089
PfoI T'CCnGG_A
Cuts at: 0 722 1290
Size: 722 568
PpuMI rG'GwC_Cy
Cuts at: 0 129 771 1290
Size: 129 642 519
Fragments arranged by size:
642 519 129
PshAI GACnn'nnGTC
Cuts at: 0 565 1290
Size: 565 725
PspOMI G'GGCC_C
Cuts at: 0 78 385 1290
Size: 78 307 905
Fragments arranged by size:
905 307 78
PstI C_TGCA'G
Cuts at: 0 173 423 432 1023 1290
Size: 173 250 9 591 267
Fragments arranged by size:
591 267 250 173 9
PvuII CAG'CTG
Cuts at: 0 342 418 675 1211 1290
Size: 342 76 257 536 79
Fragments arranged by size:
536 342 257 79 76
SacI G_AGCT'C
Cuts at: 0 313 1290
Size: 313 977
SanDI GG'GwC_CC
Cuts at: 0 129 1290
Size: 129 1161
SfcI C'TryA_G
Cuts at: 0 169 419 428 1019 1290
Size: 169 250 9 591 271
Fragments arranged by size:
591 271 250 169 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 47 1290
Size: 47 1243
SmaI CCC'GGG
Cuts at: 0 134 1290
Size: 134 1156
StyI C'CwwG_G
Cuts at: 0 827 1290
Size: 827 463
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 988 1290
Size: 988 302
TatI w'GTAC_w
Cuts at: 0 1178 1290
Size: 1178 112
XmaI C'CCGG_G
Cuts at: 0 132 1290
Size: 132 1158
Enzymes that do cut:
AarI AcuI AflIII AlwNI ApaI AvaI BanII BbvCI
BglI BlpI Bme1580I BmrI BpmI Bpu10I BsaI BseRI
BseYI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrFI BsrGI
BstAPI BstXI BstYI BtgI CspCI DrdI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I HaeII MmeI MslI MspA1I
NspI PasI PciI PflMI PfoI PpuMI PshAI PspOMI
PstI PvuII SacI SanDI SfcI SfiI SmaI StyI
TaqII TatI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BcgI BciVI
BclI BfrBI BglII BmgBI BmtI BplI BpuEI BsaAI
BsaBI BsaHI BsaWI BsaXI BsgI BsiEI BsiWI BsmBI
BspEI BsrBI BsrDI BssHII BssSI BstBI BstEII BstZ17I
Bsu36I BtgZI BtsI ClaI DraI DraIII EaeI EagI
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PmeI PmlI PpiI PsiI PspXI PsrI PvuI
RsrII SacII SalI SapI SbfI ScaI SexAI SfoI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TstI Tth111I XbaI XcmI XhoI XmnI
ZraI