Restriction map for
pF1KB7715
(Linear) (Six-base) MAPSORT of: KIBB7715.seq Check: 2930 from: 1 to: 1278
KIBB7715.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 673 783 1278
Size: 673 110 495
Fragments arranged by size:
673 495 110
AgeI A'CCGG_T
Cuts at: 0 290 1278
Size: 290 988
AleI CACnn'nnGTG
Cuts at: 0 553 1278
Size: 553 725
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 571 1054 1278
Size: 571 483 224
Fragments arranged by size:
571 483 224
AlwNI CAG_nnn'CTG
Cuts at: 0 39 1278
Size: 39 1239
ApaI G_GGCC'C
Cuts at: 0 731 1278
Size: 731 547
AvaI C'yCGr_G
Cuts at: 0 670 730 1180 1278
Size: 670 60 450 98
Fragments arranged by size:
670 450 98 60
BamHI G'GATC_C
Cuts at: 0 110 1278
Size: 110 1168
BanI G'GyrC_C
Cuts at: 0 712 815 1278
Size: 712 103 463
Fragments arranged by size:
712 463 103
BanII G_rGCy'C
Cuts at: 0 268 487 731 1278
Size: 268 219 244 547
Fragments arranged by size:
547 268 244 219
BbsI GAAGACnn'nnnn_
Cuts at: 0 241 954 1197 1278
Size: 241 713 243 81
Fragments arranged by size:
713 243 241 81
BciVI GTATCCnnnnn_n'
Cuts at: 0 77 1278
Size: 77 1201
BclI T'GATC_A
Cuts at: 0 437 473 617 1278
Size: 437 36 144 661
Fragments arranged by size:
661 437 144 36
BglI GCCn_nnn'nGGC
Cuts at: 0 674 854 1278
Size: 674 180 424
Fragments arranged by size:
674 424 180
Bme1580I G_kGCm'C
Cuts at: 0 731 818 1278
Size: 731 87 460
Fragments arranged by size:
731 460 87
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 760 841 958 1278
Size: 760 81 117 320
Fragments arranged by size:
760 320 117 81
Bpu10I CC'TnA_GC
Cuts at: 0 691 1278
Size: 691 587
BsaBI GATnn'nnATC
Cuts at: 0 143 616 1278
Size: 143 473 662
Fragments arranged by size:
662 473 143
BsaHI Gr'CG_yC
Cuts at: 0 767 1278
Size: 767 511
BsaWI w'CCGG_w
Cuts at: 0 8 107 290 1062 1208 1278
Size: 8 99 183 772 146 70
Fragments arranged by size:
772 183 146 99 70 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 106 1198 1278
Size: 106 1092 80
Fragments arranged by size:
1092 106 80
BseYI C'CCAG_C
Cuts at: 0 544 775 1141 1278
Size: 544 231 366 137
Fragments arranged by size:
544 366 231 137
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 823 1120 1278
Size: 823 297 158
Fragments arranged by size:
823 297 158
BsiEI CG_ry'CG
Cuts at: 0 417 764 1278
Size: 417 347 514
Fragments arranged by size:
514 417 347
BsmI GAATG_Cn'
Cuts at: 0 601 1278
Size: 601 677
Bsp1286I G_dGCh'C
Cuts at: 0 268 487 731 818 1278
Size: 268 219 244 87 460
Fragments arranged by size:
460 268 244 219 87
BspEI T'CCGG_A
Cuts at: 0 107 1062 1208 1278
Size: 107 955 146 70
Fragments arranged by size:
955 146 107 70
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 673 783 1278
Size: 673 110 495
Fragments arranged by size:
673 495 110
BsrFI r'CCGG_y
Cuts at: 0 290 759 848 1016 1159 1278
Size: 290 469 89 168 143 119
Fragments arranged by size:
469 290 168 143 119 89
BssSI C'ACGA_G
Cuts at: 0 1096 1278
Size: 1096 182
BstXI CCAn_nnnn'nTGG
Cuts at: 0 230 530 936 1278
Size: 230 300 406 342
Fragments arranged by size:
406 342 300 230
BstYI r'GATC_y
Cuts at: 0 110 1278
Size: 110 1168
Bsu36I CC'TnA_GG
Cuts at: 0 341 1278
Size: 341 937
BtgI C'CryG_G
Cuts at: 0 908 1278
Size: 908 370
DraI TTT'AAA
Cuts at: 0 189 201 1278
Size: 189 12 1077
Fragments arranged by size:
1077 189 12
DrdI GACnn_nn'nnGTC
Cuts at: 0 291 1278
Size: 291 987
EaeI y'GGCC_r
Cuts at: 0 761 1278
Size: 761 517
EagI C'GGCC_G
Cuts at: 0 761 1278
Size: 761 517
EarI CTCTTCn'nnn_
Cuts at: 0 91 341 1049 1130 1141 1278
Size: 91 250 708 81 11 137
Fragments arranged by size:
708 250 137 91 81 11
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 760 841 958 1278
Size: 760 81 117 320
Fragments arranged by size:
760 320 117 81
EcoO109I rG'GnC_Cy
Cuts at: 0 735 1176 1278
Size: 735 441 102
Fragments arranged by size:
735 441 102
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 503 1278
Size: 503 775
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 1278
Size: 487 791
MslI CAynn'nnrTG
Cuts at: 0 469 553 1096 1278
Size: 469 84 543 182
Fragments arranged by size:
543 469 182 84
MspA1I CmG'CkG
Cuts at: 0 561 899 1113 1278
Size: 561 338 214 165
Fragments arranged by size:
561 338 214 165
NaeI GCC'GGC
Cuts at: 0 761 850 1018 1161 1278
Size: 761 89 168 143 117
Fragments arranged by size:
761 168 143 117 89
NgoMIV G'CCGG_C
Cuts at: 0 759 848 1016 1159 1278
Size: 759 89 168 143 119
Fragments arranged by size:
759 168 143 119 89
NspI r_CATG'y
Cuts at: 0 66 105 1278
Size: 66 39 1173
Fragments arranged by size:
1173 66 39
PfoI T'CCnGG_A
Cuts at: 0 69 1278
Size: 69 1209
PsiI TTA'TAA
Cuts at: 0 143 1278
Size: 143 1135
PspOMI G'GGCC_C
Cuts at: 0 727 1278
Size: 727 551
PstI C_TGCA'G
Cuts at: 0 898 995 1278
Size: 898 97 283
Fragments arranged by size:
898 283 97
PvuII CAG'CTG
Cuts at: 0 1113 1278
Size: 1113 165
RsrII CG'GwC_CG
Cuts at: 0 288 1278
Size: 288 990
SapI GCTCTTCn'nnn_
Cuts at: 0 91 341 1141 1278
Size: 91 250 800 137
Fragments arranged by size:
800 250 137 91
SexAI A'CCwGG_T
Cuts at: 0 1199 1278
Size: 1199 79
SfcI C'TryA_G
Cuts at: 0 894 991 1278
Size: 894 97 287
Fragments arranged by size:
894 287 97
StuI AGG'CCT
Cuts at: 0 175 502 1278
Size: 175 327 776
Fragments arranged by size:
776 327 175
StyI C'CwwG_G
Cuts at: 0 115 1278
Size: 115 1163
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1137 1169 1278
Size: 1137 32 109
Fragments arranged by size:
1137 109 32
Tth111I GACn'n_nGTC
Cuts at: 0 1201 1278
Size: 1201 77
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 77 1278
Size: 77 1201
XmnI GAAnn'nnTTC
Cuts at: 0 808 1058 1278
Size: 808 250 220
Fragments arranged by size:
808 250 220
Enzymes that do cut:
AarI AgeI AleI AlfI AlwNI ApaI AvaI BamHI
BanI BanII BbsI BciVI BclI BglI Bme1580I BpmI
Bpu10I BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsmI Bsp1286I BspEI BspMI BsrFI BssSI BstXI BstYI
Bsu36I BtgI DraI DrdI EaeI EagI EarI Eco57MI
EcoO109I FalI MmeI MslI MspA1I NaeI NgoMIV NspI
PfoI PsiI PspOMI PstI PvuII RsrII SapI SexAI
SfcI StuI StyI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AhdI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BbeI BbvCI BcgI BfrBI BglII BlpI BmgBI
BmrI BmtI BplI BpuEI BsaI BsaAI BsaXI BsiHKAI
BsiWI BsmBI BspHI BsrBI BsrDI BsrGI BssHII BstAPI
BstBI BstEII BstZ17I BtgZI BtsI ClaI CspCI DraIII
EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MscI NarI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PspXI PsrI PvuI SacI SacII SalI
SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII
TatI XbaI XhoI XmaI ZraI