Restriction map for
pF1KB7711
(Linear) (Six-base) MAPSORT of: KIBB7711.seq Check: 5799 from: 1 to: 1263
KIBB7711.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AfeI AGC'GCT
Cuts at: 0 699 1263
Size: 699 564
AflIII A'CryG_T
Cuts at: 0 1020 1263
Size: 1020 243
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 415 892 1263
Size: 415 477 371
Fragments arranged by size:
477 415 371
AlwNI CAG_nnn'CTG
Cuts at: 0 114 747 750 831 1175 1263
Size: 114 633 3 81 344 88
Fragments arranged by size:
633 344 114 88 81 3
ApaLI G'TGCA_C
Cuts at: 0 282 974 1263
Size: 282 692 289
Fragments arranged by size:
692 289 282
ApoI r'AATT_y
Cuts at: 0 121 498 1263
Size: 121 377 765
Fragments arranged by size:
765 377 121
AvaI C'yCGr_G
Cuts at: 0 24 159 211 303 548 707 1263
Size: 24 135 52 92 245 159 556
Fragments arranged by size:
556 245 159 135 92 52 24
BanI G'GyrC_C
Cuts at: 0 166 192 207 1263
Size: 166 26 15 1056
Fragments arranged by size:
1056 166 26 15
BanII G_rGCy'C
Cuts at: 0 77 695 1000 1012 1193 1263
Size: 77 618 305 12 181 70
Fragments arranged by size:
618 305 181 77 70 12
BbeI G_GCGC'C
Cuts at: 0 211 1263
Size: 211 1052
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1048 1082 1263
Size: 1048 34 181
Fragments arranged by size:
1048 181 34
BglI GCCn_nnn'nGGC
Cuts at: 0 37 306 403 412 679 1219 1263
Size: 37 269 97 9 267 540 44
Fragments arranged by size:
540 269 267 97 44 37 9
BlpI GC'TnA_GC
Cuts at: 0 994 1263
Size: 994 269
Bme1580I G_kGCm'C
Cuts at: 0 169 286 978 1263
Size: 169 117 692 285
Fragments arranged by size:
692 285 169 117
BmgBI CAC'GTC
Cuts at: 0 275 287 333 1029 1263
Size: 275 12 46 696 234
Fragments arranged by size:
696 275 234 46 12
BmrI ACTGGGnnnn_n'
Cuts at: 0 591 939 1248 1263
Size: 591 348 309 15
Fragments arranged by size:
591 348 309 15
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1019 1263
Size: 1019 244
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 1000 1009 1263
Size: 88 912 9 254
Fragments arranged by size:
912 254 88 9
BsaI GGTCTCn'nnnn_
Cuts at: 0 1114 1263
Size: 1114 149
BsaAI yAC'GTr
Cuts at: 0 357 1023 1263
Size: 357 666 240
Fragments arranged by size:
666 357 240
BsaHI Gr'CG_yC
Cuts at: 0 80 208 947 1263
Size: 80 128 739 316
Fragments arranged by size:
739 316 128 80
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 58 85 778 904 1263
Size: 58 27 693 126 359
Fragments arranged by size:
693 359 126 58 27
BseYI C'CCAG_C
Cuts at: 0 663 694 740 785 807 865 969 1204
Size: 663 31 46 45 22 58 104 235
Cuts at: 1204 1263
Size: 59
Fragments arranged by size:
663 235 104 59 58 46 45 31
22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 812 1263
Size: 812 451
BsiEI CG_ry'CG
Cuts at: 0 230 1050 1263
Size: 230 820 213
Fragments arranged by size:
820 230 213
BsiHKAI G_wGCw'C
Cuts at: 0 286 978 1263
Size: 286 692 285
Fragments arranged by size:
692 286 285
Bsp1286I G_dGCh'C
Cuts at: 0 77 169 286 695 978 1000 1012 1193
Size: 77 92 117 409 283 22 12 181
Cuts at: 1193 1263
Size: 70
Fragments arranged by size:
409 283 181 117 92 77 70 22
12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1238 1263
Size: 1238 25
BsrBI CCG'CTC
Cuts at: 0 609 620 1166 1263
Size: 609 11 546 97
Fragments arranged by size:
609 546 97 11
BsrFI r'CCGG_y
Cuts at: 0 36 949 1263
Size: 36 913 314
Fragments arranged by size:
913 314 36
BssHII G'CGCG_C
Cuts at: 0 296 1263
Size: 296 967
BstAPI GCAn_nnn'nTGC
Cuts at: 0 114 750 1175 1263
Size: 114 636 425 88
Fragments arranged by size:
636 425 114 88
BstXI CCAn_nnnn'nTGG
Cuts at: 0 843 1263
Size: 843 420
BstYI r'GATC_y
Cuts at: 0 483 1263
Size: 483 780
BtgI C'CryG_G
Cuts at: 0 177 856 1044 1263
Size: 177 679 188 219
Fragments arranged by size:
679 219 188 177
BtsI GCAGTG_nn'
Cuts at: 0 115 1176 1263
Size: 115 1061 87
Fragments arranged by size:
1061 115 87
DraIII CAC_nnn'GTG
Cuts at: 0 192 1263
Size: 192 1071
EaeI y'GGCC_r
Cuts at: 0 227 395 434 845 1047 1130 1263
Size: 227 168 39 411 202 83 133
Fragments arranged by size:
411 227 202 168 133 83 39
EagI C'GGCC_G
Cuts at: 0 227 1047 1263
Size: 227 820 216
Fragments arranged by size:
820 227 216
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 55 463 496 1263
Size: 19 36 408 33 767
Fragments arranged by size:
767 408 36 33 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 1000 1009 1263
Size: 88 912 9 254
Fragments arranged by size:
912 254 88 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1198 1263
Size: 1198 65
EcoO109I rG'GnC_Cy
Cuts at: 0 144 215 315 852 984 1078 1253 1263
Size: 144 71 100 537 132 94 175 10
Fragments arranged by size:
537 175 144 132 100 94 71 10
EcoRI G'AATT_C
Cuts at: 0 121 498 1263
Size: 121 377 765
Fragments arranged by size:
765 377 121
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 594 1263
Size: 594 669
HaeII r_GCGC'y
Cuts at: 0 211 626 701 1263
Size: 211 415 75 562
Fragments arranged by size:
562 415 211 75
KasI G'GCGC_C
Cuts at: 0 207 1263
Size: 207 1056
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1250 1263
Size: 1250 13
MscI TGG'CCA
Cuts at: 0 397 847 1132 1263
Size: 397 450 285 131
Fragments arranged by size:
450 397 285 131
MslI CAynn'nnrTG
Cuts at: 0 391 397 541 1263
Size: 391 6 144 722
Fragments arranged by size:
722 391 144 6
MspA1I CmG'CkG
Cuts at: 0 205 720 747 831 858 1263
Size: 205 515 27 84 27 405
Fragments arranged by size:
515 405 205 84 27 27
NaeI GCC'GGC
Cuts at: 0 38 951 1263
Size: 38 913 312
Fragments arranged by size:
913 312 38
NarI GG'CG_CC
Cuts at: 0 208 1263
Size: 208 1055
NgoMIV G'CCGG_C
Cuts at: 0 36 949 1263
Size: 36 913 314
Fragments arranged by size:
913 314 36
PasI CC'CwG_GG
Cuts at: 0 1154 1263
Size: 1154 109
PmlI CAC'GTG
Cuts at: 0 357 1023 1263
Size: 357 666 240
Fragments arranged by size:
666 357 240
PpuMI rG'GwC_Cy
Cuts at: 0 215 852 1263
Size: 215 637 411
Fragments arranged by size:
637 411 215
PstI C_TGCA'G
Cuts at: 0 110 119 314 1148 1235 1263
Size: 110 9 195 834 87 28
Fragments arranged by size:
834 195 110 87 28 9
PvuII CAG'CTG
Cuts at: 0 747 1263
Size: 747 516
SacII CC_GC'GG
Cuts at: 0 859 1263
Size: 859 404
SanDI GG'GwC_CC
Cuts at: 0 215 1263
Size: 215 1048
SbfI CC_TGCA'GG
Cuts at: 0 1235 1263
Size: 1235 28
SexAI A'CCwGG_T
Cuts at: 0 188 1263
Size: 188 1075
SfcI C'TryA_G
Cuts at: 0 106 115 310 1144 1231 1263
Size: 106 9 195 834 87 32
Fragments arranged by size:
834 195 106 87 32 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 403 412 1263
Size: 403 9 851
Fragments arranged by size:
851 403 9
SfoI GGC'GCC
Cuts at: 0 209 1263
Size: 209 1054
SmaI CCC'GGG
Cuts at: 0 161 213 305 709 1263
Size: 161 52 92 404 554
Fragments arranged by size:
554 404 161 92 52
StyI C'CwwG_G
Cuts at: 0 140 848 1263
Size: 140 708 415
Fragments arranged by size:
708 415 140
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 677 961 1263
Size: 677 284 302
Fragments arranged by size:
677 302 284
XmaI C'CCGG_G
Cuts at: 0 159 211 303 707 1263
Size: 159 52 92 404 556
Fragments arranged by size:
556 404 159 92 52
Enzymes that do cut:
AfeI AflIII AlfI AlwNI ApaLI ApoI AvaI BanI
BanII BbeI BcgI BglI BlpI Bme1580I BmgBI BmrI
BplI BpmI BsaI BsaAI BsaHI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI
BstXI BstYI BtgI BtsI DraIII EaeI EagI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII KasI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV PasI PmlI
PpuMI PstI PvuII SacII SanDI SbfI SexAI SfcI
SfiI SfoI SmaI StyI TaqII XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AgeI
AhdI AleI AloI ApaI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BbvCI BciVI BclI BfrBI BglII
BmtI Bpu10I BpuEI BsaBI BsaWI BsaXI BsiWI BsmI
BsmBI BspEI BspHI BsrDI BsrGI BssSI BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EarI
EcoICRI EcoRV FseI FspI FspAI Hin4I HincII HindIII
HpaI KpnI MfeI MluI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SalI SapI ScaI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI TstI
Tth111I XbaI XcmI XhoI XmnI ZraI