(Linear) (Six-base) MAPSORT of: KIBB7709.seq  Check: 6782  from: 1  to: 1260



KIBB7709.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:24  ..

AccI GT'mk_AC

Cuts at:      0    1100    1260 
   Size:       1100     160
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73    1260 
   Size:         73    1187
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     924    1260 
   Size:        924     336
 
AlwNI CAG_nnn'CTG

Cuts at:      0     772    1260 
   Size:        772     488
 
ApoI r'AATT_y

Cuts at:      0     125    1260 
   Size:        125    1135
 
AseI AT'TA_AT

Cuts at:      0     493    1260 
   Size:        493     767
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     691    1260 
   Size:        691     569
 
BclI T'GATC_A

Cuts at:      0     159    1260 
   Size:        159    1101
 
BglII A'GATC_T

Cuts at:      0      81     948    1260 
   Size:         81     867     312

  Fragments arranged by size: 

                 867     312      81
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     760    1260 
   Size:        760     500
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     840    1063    1260 
   Size:        840     223     197

  Fragments arranged by size: 

                 840     223     197
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     700    1260 
   Size:        700     560
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     708    1260 
   Size:        708     552
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     513    1029    1260 
   Size:        513     516     231

  Fragments arranged by size: 

                 516     513     231
 
BseYI C'CCAG_C

Cuts at:      0     237    1260 
   Size:        237    1023
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1084    1260 
   Size:       1084     176
 
BsmI GAATG_Cn'

Cuts at:      0     279    1260 
   Size:        279     981
 
BsrDI GCAATG_nn'

Cuts at:      0     918    1260 
   Size:        918     342
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     571    1260 
   Size:        571     689
 
BstYI r'GATC_y

Cuts at:      0      81     602     948    1260 
   Size:         81     521     346     312

  Fragments arranged by size: 

                 521     346     312      81
 
BtsI GCAGTG_nn'

Cuts at:      0     516    1260 
   Size:        516     744
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     806    1260 
   Size:        806     454
 
EarI CTCTTCn'nnn_

Cuts at:      0     452    1260 
   Size:        452     808
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     840    1063    1260 
   Size:         73     767     223     197

  Fragments arranged by size: 

                 767     223     197      73
 
EcoO109I rG'GnC_Cy

Cuts at:      0     462     738    1245    1260 
   Size:        462     276     507      15

  Fragments arranged by size: 

                 507     462     276      15
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     556    1260 
   Size:        556     704
 
HaeII r_GCGC'y

Cuts at:      0     701    1260 
   Size:        701     559
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     358     390    1147    1179    1260 
   Size:        358      32     757      32      81

  Fragments arranged by size: 

                 757     358      81      32      32
 
HincII GTy'rAC

Cuts at:      0    1101    1191    1260 
   Size:       1101      90      69

  Fragments arranged by size: 

                1101      90      69
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     816    1260 
   Size:        816     444
 
NdeI CA'TA_TG

Cuts at:      0     213    1260 
   Size:        213    1047
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     138    1260 
   Size:        138    1122
 
PfoI T'CCnGG_A

Cuts at:      0    1040    1071    1260 
   Size:       1040      31     189

  Fragments arranged by size: 

                1040     189      31
 
PpuMI rG'GwC_Cy

Cuts at:      0     462     738    1260 
   Size:        462     276     522

  Fragments arranged by size: 

                 522     462     276
 
SalI G'TCGA_C

Cuts at:      0    1099    1260 
   Size:       1099     161
 
ScaI AGT'ACT

Cuts at:      0     225    1260 
   Size:        225    1035
 
SfcI C'TryA_G

Cuts at:      0     251     257    1260 
   Size:        251       6    1003

  Fragments arranged by size: 

                1003     251       6
 
SmlI C'TyrA_G

Cuts at:      0     679    1260 
   Size:        679     581
 
SspI AAT'ATT

Cuts at:      0    1026    1260 
   Size:       1026     234
 
StyI C'CwwG_G

Cuts at:      0     137    1260 
   Size:        137    1123
 
TatI w'GTAC_w

Cuts at:      0     223    1260 
   Size:        223    1037
 

 Enzymes that do cut:

     AccI     AcuI     AlfI    AlwNI     ApoI     AseI     BbsI     BclI
    BglII     BmrI     BpmI    BpuEI     BsaI    BseRI    BseYI     BsgI
     BsmI    BsrDI   BstAPI    BstYI     BtsI     DrdI     EarI  Eco57MI
 EcoO109I     FalI    HaeII    Hin4I   HincII     MmeI     NdeI    PflMI
     PfoI    PpuMI     SalI     ScaI     SfcI     SmlI     SspI     StyI
     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI
    BciVI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmtI     BplI
   Bpu10I    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI  BsiHKAI
    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     EaeI     EagI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII    SanDI     SapI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI